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AB86050

Anti-PLRG1 antibody

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(2 Publications)

Rabbit Polyclonal PLRG1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PLRG1 aa 450 to C-terminus.

View Alternative Names

Pleiotropic regulator 1, PLRG1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLRG1 antibody (AB86050)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLRG1 antibody (AB86050)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and breast carcinoma (right) tissues labelling PLRG1 with ab86050 at 1/200 (1µg/ml). Detection : DAB and a DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100).

Immunoprecipitation - Anti-PLRG1 antibody (AB86050)
  • IP

Unknown

Immunoprecipitation - Anti-PLRG1 antibody (AB86050)

Detection of PLRG1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86050 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-PLRG1 antibody (ab86050)

Predicted band size: 57 kDa

false

Western blot - Anti-PLRG1 antibody (AB86050)
  • WB

Unknown

Western blot - Anti-PLRG1 antibody (AB86050)

All lanes:

Western blot - Anti-PLRG1 antibody (ab86050) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 57 kDa

Observed band size: 45 kDa,57 kDa

true

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PLRG1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43660

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 28076346, PubMed : 28502770). Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing (PubMed : 11101529, PubMed : 11544257). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
See full target information PLRG1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:315 PubMed39747150

2025

CDC40 suppression induces CDCA5 splicing defects and anti-proliferative effects in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Die Hu,Brigitte L Thériault,Vida Talebian,Laurent Hoffer,Julie Owen,Justin Lim,Benjamin J Blencowe,Evelyne Lima-Fernandes,Punit Saraon,Richard Marcellus,Rima Al-Awar

Nucleic acids research 43:7058-69 PubMed26130721

2015

CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex.

Applications

WB

Species

Mouse

Febe van Maldegem,Sarah Maslen,Christopher M Johnson,Anita Chandra,Karuna Ganesh,Mark Skehel,Cristina Rada
View all publications
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Product promise

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