Anti-PLS1 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PLS1 antibody (AB236976)

A549 (human lung carcinoma cell line) cells stained for PLS1 (green) using ab236976 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counterstained wit DAPI.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLS1 antibody (AB236976)

Paraffin-embedded human small intestine tissue stained for PLS1 using ab236976 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLS1 antibody (AB236976)

Paraffin-embedded human kidney tissue stained for PLS1 using ab236976 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• WB

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Western blot - Anti-PLS1 antibody (AB236976)

All lanes:

Western blot - Anti-PLS1 antibody (ab236976) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

A549 (human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 70 kDa

false

• IP

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Immunoprecipitation - Anti-PLS1 antibody (AB236976)

PLS1 was immunoprecipitated from 500 μg HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab236976 at 1/200 dilution.

Lane 1 : Rabbit control IgG IP in HEK-293 whole cell lysate.

Lane 2 : ab236976 IP in HEK-293 whole cell lysate.

Lane 3 : HEK-293 whole cell lysate 20 μg (Input).

For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-PLS1 antibody (ab236976)

Predicted band size: 70 kDa

false