AB137128

Anti-PLSCR3 antibody [EPR9242]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal PLSCR3 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

Phospholipid scramblase 3, PL scramblase 3, Ca(2+)-dependent phospholipid scramblase 3, PLSCR3

1 Images

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Western blot - Anti-PLSCR3 antibody [EPR9242] (AB137128)

All lanes:

Western blot - Anti-PLSCR3 antibody [EPR9242] (ab137128) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

BxPC 3 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

false

Carrier free

Anti-PLSCR3 antibody [EPR9242] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9242

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PLSCR3 also known as phospholipid scramblase 3 plays a role in the translocation of phospholipids across cell membranes. It weighs approximately 37 kDa and is closely associated with the mitoplast. This protein is found mainly in the mitochondria heart liver and skeletal muscle tissues. PLSCR3 is vital for maintaining the asymmetry of phospholipid distribution in cellular membranes important for cell viability.

Biological function summary

The activity of PLSCR3 in the mitochondria is significant in apoptosis and lipid metabolism processes. As a part of the scramblase family it collaborates with proteins in the Bcl-2 family to modulate apoptosis. This interaction is important for regulating the release of cytochrome c an important step in the intrinsic pathway of apoptosis. The phospholipid redistribution function of PLSCR3 supports the remodeling of mitochondrial membranes during metabolic processes.

Pathways

PLSCR3 influences the apoptosis and phospholipid signaling pathways. It interacts with proteins such as Bax and Bak within the apoptosis pathway facilitating the proper execution of cell death during stress responses. In the phospholipid signaling pathway PLSCR3 coordinates with phosphatidylserine and other lipids to regulate cellular signaling processes and membrane dynamics affecting cellular responses to environmental changes.

Mutations or dysregulation of PLSCR3 can contribute to metabolic disorders and certain types of cancer. The protein's role in apoptosis links it to disorders like cardiomyopathy where regulated cell death is important for heart muscle maintenance. Additionally in cancer abnormal PLSCR3 activity impacts cell growth and survival connecting it to oncogenes like MYC which can drive uncontrolled cell proliferation. Understanding PLSCR3's role helps develop potential therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of the phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer membrane of the mitochondria (PubMed : 14573790, PubMed : 17226776, PubMed : 18358005, PubMed : 29337693, PubMed : 31769662). Plays an important role in mitochondrial respiratory function, morphology, and apoptotic response (PubMed : 12649167, PubMed : 14573790, PubMed : 17226776, PubMed : 18358005). Mediates the translocation of cardiolipin from the mitochondrial inner membrane to outer membrane enhancing t-Bid induced cytochrome c release and apoptosis (PubMed : 14573790, PubMed : 17226776, PubMed : 18358005). Enhances TNFSF10-induced apoptosis by regulating the distribution of cardiolipin in the mitochondrial membrane resulting in increased release of apoptogenic factors and consequent amplification of the activity of caspases (PubMed : 18491232). Regulates cardiolipin de novo biosynthesis and its resynthesis (PubMed : 16939411).

See full target information PLSCR3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed39335527

2024

ALCAT1-Mediated Pathological Cardiolipin Remodeling and PLSCR3-Mediated Cardiolipin Transferring Contribute to LPS-Induced Myocardial Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Han,Chenyang Wang,Xiaojing Feng,Li Hu,Beibei Wang,Xinyue Hu,Jing Wu

Biological & pharmaceutical bulletin 47:1136-1143 PubMed38866522

2024

Phospholipid Scramblase 1 Localizes Proximal to Sphingomyelin Synthase Isoforms but Is Not Involved in Sphingomyelin Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Hayashi,Takehiro Suzuki,Naoto Horioka,Naoshi Dohmae,Takashi Tanikawa

Immunity 56:2523-2541.e8 PubMed37924812

2023

Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Miao,Cong Jiang,Winston Y Chang,Haiwei Zhang,Jinsu An,Felicia Ho,Pengcheng Chen,Han Zhang,Caroline Junqueira,Dulguun Amgalan,Felix G Liang,Junbing Zhang,Charles L Evavold,Iva Hafner-Bratkovič,Zhibin Zhang,Pietro Fontana,Shiyu Xia,Markus Waldeck-Weiermair,Youdong Pan,Thomas Michel,Liron Bar-Peled,Hao Wu,Jonathan C Kagan,Richard N Kitsis,Peng Zhang,Xing Liu,Judy Lieberman

The Journal of neuroscience : the official journal 39:1930-1943 PubMed30626699

2019

Cardiolipin-Dependent Mitophagy Guides Outcome after Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Honglu Chao,Chao Lin,Qiang Zuo,Yinlong Liu,Mengqing Xiao,Xiupeng Xu,Zheng Li,Zhongyuan Bao,Huimei Chen,Yongping You,Patrick M Kochanek,Huiyong Yin,Ning Liu,Valerian E Kagan,Hülya Bayır,Jing Ji

FEBS letters 591:4056-4066 PubMed29171872

2017

Topological characterization of the mitochondrial phospholipid scramblase 3.

Applications

Unspecified application

Species

Unspecified reactive species

Luis Alberto Luévano-Martínez,Alicia J Kowaltowski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PLSCR3 antibody [EPR9242]

Western blot - Anti-PLSCR3 antibody [EPR9242] (AB137128)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise