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AB248837

Anti-PLSCR3 antibody [EPR9242] - BSA and Azide free

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Rabbit Recombinant Monoclonal PLSCR3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Phospholipid scramblase 3, PL scramblase 3, Ca(2+)-dependent phospholipid scramblase 3, PLSCR3

1 Images
Western blot - Anti-PLSCR3 antibody [EPR9242] - BSA and Azide free (AB248837)
  • WB

Unknown

Western blot - Anti-PLSCR3 antibody [EPR9242] - BSA and Azide free (AB248837)

This data was developed using ab137128, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PLSCR3 antibody [EPR9242] (<a href='/en-us/products/primary-antibodies/plscr3-antibody-epr9242-ab137128'>ab137128</a>) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

BxPC 3 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9242

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248837 is the carrier-free version of ab137128.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PLSCR3 also known as phospholipid scramblase 3 plays a role in the translocation of phospholipids across cell membranes. It weighs approximately 37 kDa and is closely associated with the mitoplast. This protein is found mainly in the mitochondria heart liver and skeletal muscle tissues. PLSCR3 is vital for maintaining the asymmetry of phospholipid distribution in cellular membranes important for cell viability.
Biological function summary

The activity of PLSCR3 in the mitochondria is significant in apoptosis and lipid metabolism processes. As a part of the scramblase family it collaborates with proteins in the Bcl-2 family to modulate apoptosis. This interaction is important for regulating the release of cytochrome c an important step in the intrinsic pathway of apoptosis. The phospholipid redistribution function of PLSCR3 supports the remodeling of mitochondrial membranes during metabolic processes.

Pathways

PLSCR3 influences the apoptosis and phospholipid signaling pathways. It interacts with proteins such as Bax and Bak within the apoptosis pathway facilitating the proper execution of cell death during stress responses. In the phospholipid signaling pathway PLSCR3 coordinates with phosphatidylserine and other lipids to regulate cellular signaling processes and membrane dynamics affecting cellular responses to environmental changes.

Mutations or dysregulation of PLSCR3 can contribute to metabolic disorders and certain types of cancer. The protein's role in apoptosis links it to disorders like cardiomyopathy where regulated cell death is important for heart muscle maintenance. Additionally in cancer abnormal PLSCR3 activity impacts cell growth and survival connecting it to oncogenes like MYC which can drive uncontrolled cell proliferation. Understanding PLSCR3's role helps develop potential therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of the phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer membrane of the mitochondria (PubMed : 14573790, PubMed : 17226776, PubMed : 18358005, PubMed : 29337693, PubMed : 31769662). Plays an important role in mitochondrial respiratory function, morphology, and apoptotic response (PubMed : 12649167, PubMed : 14573790, PubMed : 17226776, PubMed : 18358005). Mediates the translocation of cardiolipin from the mitochondrial inner membrane to outer membrane enhancing t-Bid induced cytochrome c release and apoptosis (PubMed : 14573790, PubMed : 17226776, PubMed : 18358005). Enhances TNFSF10-induced apoptosis by regulating the distribution of cardiolipin in the mitochondrial membrane resulting in increased release of apoptogenic factors and consequent amplification of the activity of caspases (PubMed : 18491232). Regulates cardiolipin de novo biosynthesis and its resynthesis (PubMed : 16939411).
See full target information PLSCR3
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Product promise

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For full details, please see our Terms & Conditions

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