Anti-PLTP antibody [EPR24448-21]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLTP antibody [EPR24448-21] (AB282456)

Immunohistochemical analysis of paraffin-embedded human liver tissue labelling PLTP with ab282456 at 1/500 (0.988 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on human liver.

The section was incubated with ab282456 overnight at 4°C. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLTP antibody [EPR24448-21] (AB282456)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labelling PLTP with ab282456 at 1/500 (0.988 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta. (PMID : 7654777, 22090281). The section was incubated with ab282456 overnight at 4°C. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLTP antibody [EPR24448-21] (AB282456)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling PLTP with ab282456 at 1/500 (0.988 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum. The section was incubated with ab282456 for overnight at 4°C. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLTP antibody [EPR24448-21] (AB282456)

Immunohistochemical analysis of paraffin-embedded human thymus tissue labelling PLTP with ab282456 at 1/500 (0.988 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human thymus (PMID : 7654777). The section was incubated with ab282456 overnight at 4°C. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-PLTP antibody [EPR24448-21] (AB282456)

PLTP was immunoprecipitated from 0.35 mg human placenta tissue lysate 10 μg with ab282456 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab282456 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human placenta tissue lysate 10 μg

Lane 2 : ab282456 IP in human placenta tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab282456 in Human placenta tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-PLTP antibody [EPR24448-21] (ab282456)

Predicted band size: 55 kDa

Observed band size: 80 kDa

false

• WB

Lab

Western blot - Anti-PLTP antibody [EPR24448-21] (AB282456)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 26 seconds

All lanes:

Western blot - Anti-PLTP antibody [EPR24448-21] (ab282456) at 1/1000 dilution

All lanes:

His-tagged human PLTP recombinant protein (aa 18-493) at 0.05 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 55 kDa

false

• WB

Lab

Western blot - Anti-PLTP antibody [EPR24448-21] (AB282456)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 15342679, 19321130, 11013307, 7654777). High expression : plasma (PMID : 15342679, 11013307). Low expression : liver and skeletal muscle (PMID : 7654777). Bands of 80KDa and 70KDa respectively represent the extracellular form and intracellular form. Exposure time : Lanes 1-4 : 59 seconds; Lane 5 : 10 seconds.

All lanes:

Western blot - Anti-PLTP antibody [EPR24448-21] (ab282456) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

JAR (human placenta choriocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Human plasma at 5 µL

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 55 kDa

Observed band size: 70-80 kDa

false