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Rabbit Recombinant Monoclonal PLTP antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-PLTP antibody [EPR24448-48] - BSA and Azide free (Capture) (AB285012), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Mediates the transfer of phospholipids and free cholesterol from triglyceride-rich lipoproteins (low density lipoproteins or LDL and very low density lipoproteins or VLDL) into high-density lipoproteins (HDL) as well as the exchange of phospholipids between triglyceride-rich lipoproteins themselves (PubMed:11013307, PubMed:19321130, PubMed:21515415, PubMed:29883800, PubMed:7654777, PubMed:9132017). Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine (PubMed:9132017). Plays an important role in HDL remodeling which involves modulating the size and composition of HDL (PubMed:29883800). Also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion (PubMed:21736953). Two distinct forms of PLTP exist in plasma: an active form that can transfer phosphatidylcholine from phospholipid vesicles to HDL, and an inactive form that lacks this capability (PubMed:11013307).

Alternative names

Phospholipid transfer protein, Lipid transfer protein II, PLTP

Recommended products

Rabbit Recombinant Monoclonal PLTP antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR24448-48
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab285012 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PLTP. The recommended pair for sandwich ELISA is:
Capture: ab285012, Human PLTP Capture Antibody (unconjugated)
Detector: Anti-PLTP antibody [EPR24448-165] - BSA and Azide free (Detector) ab285118, Human PLTP Detector Antibody (unconjugated)
The reference range value is 19 - 5000 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PLTP also known as Phospholipid Transfer Protein is an important component in human lipid metabolism. This protein functions by transferring phospholipids between different types of lipoproteins in plasma. PLTP possesses a molecular mass of about 56 kDa. Its expression occurs mainly in the liver and adipose tissue with additional presence in the brain and lungs. These locales of expression facilitate its key roles in lipid processing and lipoprotein metabolism.

Biological function summary

PLTP plays a significant role in remodeling high-density lipoproteins (HDL) and regulating lipoprotein particles. PLTP exists as a part of a larger complex involved in these processes interacting closely with other proteins in the lipoprotein maturation and transformation. This activity of PLTP significantly affects the levels and sizes of HDL particles in circulation thereby influencing cholesterol homeostasis within the body.

Pathways

PLTP acts within the reverse cholesterol transport pathway important for the removal of excess cholesterol from peripheral tissues. The protein works alongside Apolipoprotein A-I (ApoA-I) and Lecithin-Cholesterol Acyltransferase (LCAT) to promote cholesterol efflux and HDL maturation. Another key pathway involving PLTP is the lipid metabolism pathway where it influences lipid levels and impacts the function of other proteins such as Apolipoprotein E (ApoE).

Associated diseases and disorders

PLTP has associations with atherosclerosis and metabolic syndrome. Variations in PLTP activity or expression can lead to altered HDL levels increasing the risk for heart disease and related cardiovascular conditions. In atherosclerosis PLTP's relationship with ApoA-I becomes especially relevant as this interaction influences lipid transport dynamics and plaque formation. Additionally in metabolic syndrome abnormal PLTP function may contribute to dyslipidemia a common factor in metabolic disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Anti-PLTP antibody [EPR24448-48] - BSA and Azide free (Capture) (ab285012), expandable thumbnail

    Sandwich ELISA - Anti-PLTP antibody [EPR24448-48] - BSA and Azide free (Capture) (ab285012)

    Example of Human PLTP standard curve. Background-subtracted data values (mean +/- SD) are graphed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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