Rabbit Recombinant Monoclonal PLVAP/PV-1 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples.
Images
![Flow Cytometry - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (AB321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--flow-cytometry-img436260.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (AB321890)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (AB321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436225.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (AB321890)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (AB321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436224.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (AB321890)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (AB321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436223.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (AB321890)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (AB321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436222.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (AB321890)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Target data
Function
Endothelial cell-specific membrane protein involved in the formation of the diaphragms that bridge endothelial fenestrae. It is also required for the formation of stomata of caveolae and transendothelial channels. Functions in microvascular permeability, endothelial fenestrae contributing to the passage of water and solutes and regulating transcellular versus paracellular flow in different organs. Plays a specific role in embryonic development.
Alternative names
FELS, PV1, PLVAP, Plasmalemma vesicle-associated protein, Fenestrated endothelial-linked structure protein, Plasmalemma vesicle protein 1, PV-1
Recommended products
Rabbit Recombinant Monoclonal PLVAP/PV-1 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29045-558
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab321890 is the carrier-free version of Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PLVAP also known as PV-1 is a transmembrane protein that plays a significant role in organizing the stomatal and fenestral diaphragms of endothelial cells. It weighs approximately 60 kDa. This protein is mainly expressed in fenestrated capillaries and sinusoidal endothelium where it forms diaphragms and contributes to the permeability and ultrastructure of capillary beds. PLVAP's role in endothelial cells highlights its importance in maintaining vascular permeability and cellular structures.
- Biological function summary
The functions of PLVAP are significant in maintaining an organized structure within endothelial cells. It forms part of the complex structure that comprises the diaphragms of endothelial fenestrae transendothelial channels and caveolae. These structures are necessary for regulating fluid exchange and molecular transport between blood and tissue. The presence of PLVAP at these sites signals its role in modulating vascular barrier functions essential for efficient blood-tissue communication.
- Pathways
Researchers have identified PLVAP's involvement in important vascular and endothelial pathways. It plays a critical part in the VEGF signaling pathway which regulates vascular permeability and endothelial cell behavior. PLVAP is closely associated with other proteins like VE-cadherin and claudin-5 that ensure cohesion and permeability in endothelial cells. In pathways regulating vascular permeability PLVAP's presence helps mediate fluid exchange and maintain tissue homeostasis.
- Associated diseases and disorders
PLVAP associates with conditions involving vascular dysregulation. Hypo- or hyper-expression of PLVAP can relate to the pathogenesis of cancer and inflammatory diseases. In cancer changes in PLVAP expression might contribute to altered tumor vasculature and metastasis often in association with proteins like VEGF and VEGFR-2. Furthermore inflammatory diseases such as atherosclerosis see changes in the protein expression levels that might disrupt vascular integrity linking them with proteins involved in inflammatory responses like ICAM-1 and VCAM-1.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Flow Cytometry - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--flow-cytometry-img436260.jpg "Flow Cytometry - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)")
Flow Cytometry - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)
This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte, Left) / TF-1 (human erythroleukemia erythroblast, Right).
Cells were labelled with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 at 1/5000 dilution (0.01 µg) (red), or with Rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black), or cells were unlabelled (blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436225.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)
This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) TF-1 (human erythroleukemia erythroblast) cell pellet; (B) Raji (human Burkitt's lymphoma B lymphocyte) cell pellet tissue labeling PLVAP/PV-1 with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) TF-1 cell pellet; no staining on (B) Raji cell pellet.
The section was incubated with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436224.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)
This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PLVAP/PV-1 with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: weak staining on endothelium of human skeletal muscle.
The section was incubated with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436223.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)
This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colorectal carcinoma tissue labeling PLVAP/PV-1 with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of human colorectal carcinoma (PMID: 31419514).
The section was incubated with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436222.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)
This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PLVAP/PV-1 with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of human kidney (PMID: 16099878).
The section was incubated with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436221.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)
This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human appendix tissue labeling PLVAP/PV-1 with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on endothelium of human appendix (PMID: 31419514).
The section was incubated with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunocytochemistry-immunofluorescence-img436207.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)")
Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)
This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized TF-1 (human erythroleukemia erythroblast) cells labelling PLVAP/PV-1 with Anti-PLVAP/PV-1 antibody [EPR29045-558] ab321889 at 1/50 (10.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic with membranous staining in TF-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control: Raji
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436221.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)")
![Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890), expandable thumbnail](https://content.abcam.com/products/images/plvap-pv-1-antibody-epr29045-558-bsa-and-azide-free-ab321890--immunocytochemistry-immunofluorescence-img436207.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [EPR29045-558] - BSA and Azide free (ab321890)")
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