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Rabbit Recombinant Monoclonal PLVAP/PV-1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (AB323707), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (AB323707), expandable thumbnail
  • Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (AB323707), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (AB323707), expandable thumbnail
  • Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (AB323707), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow CytICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Endothelial cell-specific membrane protein involved in the formation of the diaphragms that bridge endothelial fenestrae. It is also required for the formation of stomata of caveolae and transendothelial channels. Functions in microvascular permeability, endothelial fenestrae contributing to the passage of water and solutes and regulating transcellular versus paracellular flow in different organs. Plays a specific role in embryonic development.

Alternative names

FELS, PV1, PLVAP, Plasmalemma vesicle-associated protein, Fenestrated endothelial-linked structure protein, Plasmalemma vesicle protein 1, PV-1

Rabbit Recombinant Monoclonal PLVAP/PV-1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR29045-583
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab323707 is the carrier-free version of Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (ab323707), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (ab323707)

    This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labeling PLVAP/PV-1 with Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706 at 1/2000 (0.239 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive on endothelial cells in human cervical squamous carcinoma. The section was incubated with Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (ab323707), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (ab323707)

    This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling PLVAP/PV-1 with Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706 at 1/2000 (0.239 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive on endothelial cells in human clear cell carcinoma. The section was incubated with Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (ab323707), expandable thumbnail

    Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (ab323707)

    This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: cerebellum, testis(PMID: 11401446)

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30854484).

    The identity of the bands lower than 70 kDa in lanes 1-2 are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] (Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706) at 1/1000 dilution

    Lane 1: Human pancreas tissue lysate at 20 µg

    Lane 2: Human lymph node tissue lysate at 20 µg

    Lane 3: Human lung cancer tissue lysate at 20 µg

    Lane 4: Human lung tissue lysate at 20 µg

    Lane 5: Human cerebellum tissue lysate at 20 µg

    Lane 6: Human testis tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 70 kDa, 36 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (ab323707), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (ab323707)

    This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PLVAP/PV-1 with Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706 at 1/2000 (0.239 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive on endothelial cells in human colon (PMID: 31419514). The section was incubated with Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (ab323707), expandable thumbnail

    Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] - BSA and Azide free (ab323707)

    This data was developed using Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Raji, MCF7.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PLVAP/PV-1 antibody [EPR29045-583] (Anti-PLVAP/PV-1 antibody [EPR29045-583] ab323706) at 1/1000 dilution

    Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Lane 2: TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg

    Lane 3: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 70 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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