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AB104854

Anti-Plzf antibody [5B3]

2

(2 Reviews)

|

(31 Publications)

Mouse Monoclonal Plzf antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 31 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZBTB16.

View Alternative Names

PLZF, ZNF145, ZBTB16, Zinc finger and BTB domain-containing protein 16, Promyelocytic leukemia zinc finger protein, Zinc finger protein 145, Zinc finger protein PLZF

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Plzf antibody [5B3] (AB104854)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Plzf antibody [5B3] (AB104854)

Immunofluorescence analysis of Hela cells using ab104854 (green). Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry - Anti-Plzf antibody [5B3] (AB104854)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Plzf antibody [5B3] (AB104854)

Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Western blot - Anti-Plzf antibody [5B3] (AB104854)
  • WB

Unknown

Western blot - Anti-Plzf antibody [5B3] (AB104854)

All lanes:

Western blot - Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution

All lanes:

HeLa cell lysate

Predicted band size: 58 kDa,74 kDa,82 kDa,88 kDa

Observed band size: 105 kDa,140 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5B3

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ZBTB16. The exact immunogen used to generate this antibody is proprietary information.

Q05516

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

This product was changed from ascites to supernatant. Lot no's high than GR161367-22 are from Tissue Culture Supernatant
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plzf also known as promyelocytic leukemia zinc finger protein is a transcriptional repressor critical for its mechanical role in gene regulation. It has a mass of approximately 75 kDa. PLZF shows presence in various tissues notably in hematopoietic stem cells testis and certain neural tissues. It functions by binding to specific DNA sequences influencing transcriptional activity. This protein is part of the large BTB/POZ zinc finger family and it plays an engaged role in chromatin remodeling.
Biological function summary

Plzf helps regulate cell differentiation proliferation and self-renewal. Plzf works within several complexes to control gene expression in hematopoietic and germline stem cells. Through these activities it maintains stem cell quiescence and properly times cell differentiation. Plzf impacts neural crest cell fate during embryogenesis and participates in spermatogonia renewal. This involvement in various cellular processes makes it an important protein for development and stem cell biology.

Pathways

Plzf plays a role in pathways such as the Notch signaling and retinoic acid receptor pathways. Within these pathways Plzf interacts with proteins like RAR (retinoic acid receptor) and Hes-1. These interactions affect transcriptional responses that balance stem cell maintenance and differentiation. Plzf's presence in these pathways underlines its importance in controlling genetic programs associated with development and cellular growth.

Plzf associates closely with acute promyelocytic leukemia (APL) and spermatogenic failure. In APL a specific translocation involving the PLZF gene and the retinoic acid receptor alpha (RARA) gene leads to an oncogenic fusion protein disrupting normal myeloid differentiation. This disruption contributes directly to leukemia pathogenesis. In spermatogenic failure abnormal expression or mutation of Plzf impacts testicular function leading to infertility. Studies examine these connections to understand potential therapeutic targets and treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional repressor (PubMed : 10688654, PubMed : 24359566). Transcriptional repression may be mediated through recruitment of histone deacetylases to target promoters (PubMed : 10688654). May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 14528312).
See full target information ZBTB16
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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Iranian journal of basic medical sciences 28:1107-1118 PubMed40584446

2025

Testicular extracellular matrix/gelatin-based scaffold using gas foaming to support spermatogonial stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Momeni,Mohammad Kazemi Ashtiani,Zahra Bashiri,Zohreh Bagher,Hamidreza Asgari,Morteza Koruji

CytoJournal 22:37 PubMed40469708

2025

Zinc finger and broad-complex, tramtrack, and bric-a-brac domain containing 16 silencing attenuates bleomycin-induced pulmonary fibrosis in mice through inhibition of the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiansong Fang,Xiaoyun Wen,Liang Zhou,Yingjie Jiang,Liefeng Wang

Cell journal 26:611-618 PubMed40007447

2025

Effect of Metformin on Spermatogenesis Restoration Following Spermatogonial Stem Cell Transplantation in An Azoospermia Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Azim Hedayatpour,Peyman Modarresi,Shokoofeh Kazemzadeh,Alieh Bashghareh

Acta biochimica et biophysica Sinica 56:1633-1643 PubMed39308302

2024

knockdown induces cell cycle arrest and apoptosis in breast cancer cells through the ZBTB16/ANXA7/Cyclin B1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Di Liu,Qin Du,Yuxuan Zhu,Yize Guo,Ya Guo

Journal of assisted reproduction and genetics 41:2079-2098 PubMed38839698

2024

The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Rahbar,Reza Asadpour,Zohreh Mazaheri

Galen medical journal 12:e2805 PubMed38774852

2024

Evaluation of Apoptosis-related Genes and Hormone Secretion Profiles Using Three Dimensional Culture System of Human Testicular Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Aghbibi Nikmahzar,Farnaz Khadivi,Morteza Koruji,Mehrdad Jahanshahi,Masoomeh Dehghan Tarazjani,Maryam Shabani,Yasaman Abbasi,Mehdi Abbasi

Macromolecular bioscience 24:e2300342 PubMed37729950

2023

Polycaprolactone/Testicular Extracellular Matrix/Graphene Oxide-Based Electrospun Tubular Scaffolds for Reproductive Medicine: Biomimetic Architecture of Seminiferous Tubules.

Applications

Unspecified application

Species

Unspecified reactive species

Amirhossein Mohammadi,Morteza Koruji,Mahmoud Azami,Ronak Shabani,Sanam Mohandesnezhad,Zahra Bashiri,Hamidreza Asgari

Artificial organs 47:1818-1830 PubMed37698035

2023

Differentiation of human primary testicular cells in the presence of SCF using the organoid culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Aghbibi Nikmahzar,Morteza Koruji,Mehrdad Jahanshahi,Farnaz Khadivi,Maryam Shabani,Sanaz Dehghani,Mehdi Forouzesh,Ayob Jabari,Narjes Feizollahi,Maryam Salem,Nasrin Ghanami Gashti,Yasaman Abbasi,Mehdi Abbasi

Autophagy 19:2934-2957 PubMed37450577

2023

Autophagy regulation and protein kinase activity of PIK3C3 controls sertoli cell polarity through its negative regulation on SCIN (scinderin).

Applications

Unspecified application

Species

Unspecified reactive species

Kehan Wang,Feifei Kong,Yuexin Qiu,Tao Chen,Jiayi Fu,Xin Jin,Youqiang Su,Yayun Gu,Zhibin Hu,Jing Li

Bone research 11:30 PubMed37280207

2023

Super enhancers targeting ZBTB16 in osteogenesis protect against osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhui Yu,Zhongyu Xie,Jinteng Li,Jiajie Lin,Zepeng Su,Yunshu Che,Feng Ye,Zhaoqiang Zhang,Peitao Xu,Yipeng Zeng,Xiaojun Xu,Zhikun Li,Pei Feng,Rujia Mi,Yanfeng Wu,Huiyong Shen
View all publications
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Product promise

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