Rabbit Recombinant Monoclonal Plzf antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (AB305065), expandable thumbnail](https://content.abcam.com/products/images/plzf-antibody-epr24830-8-bsa-and-azide-free-ab305065--western-blot-img403498.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (AB305065)")
![Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (AB305065), expandable thumbnail](https://content.abcam.com/products/images/plzf-antibody-epr24830-8-bsa-and-azide-free-ab305065--western-blot-img403497.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (AB305065)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | ChIP | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Acts as a transcriptional repressor (PubMed:10688654, PubMed:24359566). Transcriptional repression may be mediated through recruitment of histone deacetylases to target promoters (PubMed:10688654). May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14528312).
Alternative names
PLZF, ZNF145, ZBTB16, Zinc finger and BTB domain-containing protein 16, Promyelocytic leukemia zinc finger protein, Zinc finger protein 145, Zinc finger protein PLZF
Recommended products
Rabbit Recombinant Monoclonal Plzf antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24830-8
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plzf also known as promyelocytic leukemia zinc finger protein is a transcriptional repressor critical for its mechanical role in gene regulation. It has a mass of approximately 75 kDa. PLZF shows presence in various tissues notably in hematopoietic stem cells testis and certain neural tissues. It functions by binding to specific DNA sequences influencing transcriptional activity. This protein is part of the large BTB/POZ zinc finger family and it plays an engaged role in chromatin remodeling.
- Biological function summary
Plzf helps regulate cell differentiation proliferation and self-renewal. Plzf works within several complexes to control gene expression in hematopoietic and germline stem cells. Through these activities it maintains stem cell quiescence and properly times cell differentiation. Plzf impacts neural crest cell fate during embryogenesis and participates in spermatogonia renewal. This involvement in various cellular processes makes it an important protein for development and stem cell biology.
- Pathways
Plzf plays a role in pathways such as the Notch signaling and retinoic acid receptor pathways. Within these pathways Plzf interacts with proteins like RAR (retinoic acid receptor) and Hes-1. These interactions affect transcriptional responses that balance stem cell maintenance and differentiation. Plzf's presence in these pathways underlines its importance in controlling genetic programs associated with development and cellular growth.
- Associated diseases and disorders
Plzf associates closely with acute promyelocytic leukemia (APL) and spermatogenic failure. In APL a specific translocation involving the PLZF gene and the retinoic acid receptor alpha (RARA) gene leads to an oncogenic fusion protein disrupting normal myeloid differentiation. This disruption contributes directly to leukemia pathogenesis. In spermatogenic failure abnormal expression or mutation of Plzf impacts testicular function leading to infertility. Studies examine these connections to understand potential therapeutic targets and treatment strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (ab305065), expandable thumbnail](https://content.abcam.com/products/images/plzf-antibody-epr24830-8-bsa-and-azide-free-ab305065--western-blot-img403498.jpg "Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (ab305065)")
Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (ab305065)
This data was developed using Anti-Plzf antibody [EPR24830-8] ab305064, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBSTAll lanes: Western blot - Anti-Plzf antibody [EPR24830-8] (Anti-Plzf antibody [EPR24830-8] ab305064) at 1/1000 dilution
Lane 1: LNCaP (human prostate carcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg
Lane 2: LNCaP transfected with siRNA specifically targeti Zinc fier and BTB domain-containing protein 16, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 92s
![Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (ab305065), expandable thumbnail](https://content.abcam.com/products/images/plzf-antibody-epr24830-8-bsa-and-azide-free-ab305065--western-blot-img403497.jpg "Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (ab305065)")
Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (ab305065)
This data was developed using Anti-Plzf antibody [EPR24830-8] ab305064, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used immediately to minimize protein degradation.
Samples are non-boiled as boiling may cause protein aggregation.All lanes: Western blot - Anti-Plzf antibody [EPR24830-8] (Anti-Plzf antibody [EPR24830-8] ab305064) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 3min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com