JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB4645

Anti-Pma1 antibody [40B7]

4

(1 Review)

|

(32 Publications)

Mouse Monoclonal PMA1 antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 32 publications.

View Alternative Names

YGL008C, PMA1, Plasma membrane ATPase 1, Proton pump 1

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

40B7

Isotype

IgG

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB, ICC/IF

applications

Specificity

Reacts with Pma1p based on the size of the band detected on western blots and the immunofluorescence localization pattern.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>(cell lysates, ECL detection). For non-ECL western detection methods, 1/1000 - 1/5000. Also detects Pma1p-containing fusion proteins on western blots.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Sterile filtered.
Storage buffer
Preservative: 0.065% Sodium azide Constituents: Tissue culture supernatant
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pma1 also known as plasma membrane H+-ATPase is an essential enzyme that functions as a proton pump in cells. It maintains a proton gradient across the plasma membrane by extruding H+ ions out of the cell which is an important process for cell homeostasis. Pma1 has a molecular mass of approximately 100 kDa. It is mainly expressed in the plasma membrane of yeast cells and other fungi where it plays a critical role in regulating intracellular pH and membrane potential.
Biological function summary

The plasma membrane H+-ATPase activity of Pma1 facilitates nutrient uptake and cell growth by providing an electrochemical gradient. In yeast it forms no known complex but works in conjunction with other membrane transporters to manage ions and metabolite exchange. Through this activity Pma1 indirectly supports various cellular processes including metabolism stress responses and signal transduction.

Pathways

The proton pump action of Pma1 is integral to the regulation of osmotic balance and energy metabolism pathways. It is linked to the glycolytic pathway by influencing the uptake of glucose through transporters. Pma1 is also associated with the oxidative phosphorylation pathway by maintaining the proton motive force necessary for ATP synthesis. It interacts with proteins such as glucose transporters and ion channels to ensure proper metabolic functions.

Pma1 is linked to the understanding of fungal infections as its function is critical for fungal cell survival. Inhibiting Pma1 can be a potential therapeutic target considering its absence in mammalian cells thereby providing specificity. It also offers insight into acidophilic disorders where proton gradients are disrupted. While no direct human protein counterparts exist its functional analogy in proton pumps relates it to the broader category of proton-pump-associated conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
See full target information PMA1
style
left-column

Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 80:32 PubMed36609589

2023

HSP70-binding motifs function as protein quality control degrons.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda B Abildgaard,Vasileios Voutsinos,Søren D Petersen,Fia B Larsen,Caroline Kampmeyer,Kristoffer E Johansson,Amelie Stein,Tommer Ravid,Claes Andréasson,Michael K Jensen,Kresten Lindorff-Larsen,Rasmus Hartmann-Petersen

The Journal of cell biology 221: PubMed36112368

2022

Triglyceride lipolysis triggers liquid crystalline phases in lipid droplets and alters the LD proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Sean Rogers,Long Gui,Anastasiia Kovalenko,Valeria Zoni,Maxime Carpentier,Kamran Ramji,Kalthoum Ben Mbarek,Amelie Bacle,Patrick Fuchs,Pablo Campomanes,Evan Reetz,Natalie Ortiz Speer,Emma Reynolds,Abdou Rachid Thiam,Stefano Vanni,Daniela Nicastro,W Mike Henne

BMC biology 20:163 PubMed35840963

2022

Proton export upregulates aerobic glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Shonagh Russell,Liping Xu,Yoonseok Kam,Dominique Abrahams,Bryce Ordway,Alex S Lopez,Marilyn M Bui,Joseph Johnson,Tamir Epstein,Epifanio Ruiz,Mark C Lloyd,Pawel Swietach,Daniel Verduzco,Jonathan Wojtkowiak,Robert J Gillies

Structure (London, England : 1993) 30:1245-1253.e5 PubMed35700725

2022

Disease-linked mutations cause exposure of a protein quality control degron.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Kampmeyer,Sven Larsen-Ledet,Morten Rose Wagnkilde,Mathias Michelsen,Henriette K M Iversen,Sofie V Nielsen,Søren Lindemose,Alberto Caregnato,Tommer Ravid,Amelie Stein,Kaare Teilum,Kresten Lindorff-Larsen,Rasmus Hartmann-Petersen

PLoS genetics 17:e1009539 PubMed33914734

2021

Mapping the degradation pathway of a disease-linked aspartoacylase variant.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah K Gersing,Yong Wang,Martin Grønbæk-Thygesen,Caroline Kampmeyer,Lene Clausen,Martin Willemoës,Claes Andréasson,Amelie Stein,Kresten Lindorff-Larsen,Rasmus Hartmann-Petersen

International journal of biological macromolecules 171:343-357 PubMed33421469

2021

Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Cátia Santos-Pereira,María T Andrés,Susana R Chaves,José F Fierro,Hernâni Gerós,Stéphen Manon,Lígia R Rodrigues,Manuela Côrte-Real

Methods in molecular biology (Clifton, N.J.) 2049:165-190 PubMed31602611

2019

Mapping the Saccharomyces cerevisiae Spatial Proteome with High Resolution Using hyperLOPIT.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J H Nightingale,Stephen G Oliver,Kathryn S Lilley

Bio-protocol 9:e3303 PubMed33654815

2019

A Protocol to Map the Spatial Proteome Using HyperLOPIT in .

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J H Nightingale,Kathryn S Lilley,Stephen G Oliver

The Journal of cell biology 218:961-976 PubMed30578283

2018

Rab5 GTPases are required for optimal TORC2 function.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa N Locke,Jeremy Thorner

Environmental science and pollution research international 26:2375-2386 PubMed30467749

2018

Polychlorinated biphenyl 126 exposure in rats alters skeletal muscle mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Camille Tremblay-Laganière,Léa Garneau,Jean-François Mauger,Vian Peshdary,Ella Atlas,Alyssa Samantha Nikolla,Natalie Ann Chapados,Céline Aguer
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB113684

Anti-VMA2 antibody [13D11B2]

Western blot - Anti-VMA2 antibody [13D11B2] (AB113684)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB113683

Anti-VPH1 antibody [10D7A7B2]

Western blot - Anti-VPH1 antibody [10D7A7B2] (AB113683)

  • 3
  • 1
Primary Antibodies

AB62341

Anti-RFP antibody

Lab Essentials

Western blot - Anti-RFP antibody (AB62341)

  • 4
  • 23

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com