Anti-PMCA1 antibody [EPR12029] Rabbit monoclonal (ab190355)

Rabbit Recombinant Monoclonal PMCA1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

Related conjugates and formulations

ab251018
Carrier free
Anti-PMCA1 antibody [EPR12029] - BSA and Azide free
ab319705
Conjugated
Alexa Fluor® 594 Anti-PMCA1 antibody [EPR12029]
ab319601
Conjugated
Alexa Fluor® 647 Anti-PMCA1 antibody [EPR12029]
ab319488
Conjugated
Alexa Fluor® 488 Anti-PMCA1 antibody [EPR12029]
ab319354
Conjugated
PE Anti-PMCA1 antibody [EPR12029]
ab319206
Conjugated
APC Anti-PMCA1 antibody [EPR12029]
ab321313
Conjugated
Alexa Fluor® 750 Anti-PMCA1 antibody [EPR12029]

Images

Western blot - Anti-PMCA1 antibody [EPR12029] (AB190355), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IP
Human	Tested	Tested	Tested
Mouse	Tested	Tested	Expected
Rat	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -
Species Rat	Dilution info 1/1000 - 1/10000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information ATP2B1

Function

Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis (PubMed:35358416). Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (By similarity). Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells (PubMed:29104511). May play a role in synaptic transmission by modulating calcium and proton dynamics at the synaptic vesicles.

Alternative names

PMCA1, ATP2B1, Plasma membrane calcium-transporting ATPase 1, Plasma membrane calcium ATPase isoform 1, Plasma membrane calcium pump isoform 1

Recommended products

Rabbit Recombinant Monoclonal PMCA1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR12029
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The plasma membrane calcium ATPase 1 (PMCA1) also known by its gene name ATP2B1 is an essential protein responsible for the regulation of intracellular calcium levels. Mechanically PMCA1 functions as a calcium pump actively transporting calcium ions out of the cell using ATP hydrolysis. This protein has a molecular mass of approximately 135 kDa. PMCA1 is widely expressed in many tissues but shows higher expression in excitable cells such as neurons and muscle cells where rapid calcium signaling is critical.

Biological function summary

PMCA1 plays a fundamental role in maintaining calcium homeostasis which is important for many cellular processes including muscle contraction nerve impulse transmission and cell signaling. It functions independently but in some contexts it may interact with other proteins to modulate its activity. The protein helps prevent toxic accumulation of calcium inside cells therefore protecting cellular integrity and function.

Pathways

PMCA1 is integral to the calcium signaling pathway where it helps regulate cellular responses to fluctuating calcium levels. It interacts with other key proteins such as calmodulin which can enhance its activity in response to increased intracellular calcium. PMCA1 also links to the phosphatidylinositol signaling pathway where its function assists in the propagation of signaling cascades that manage processes like growth and metabolism.

Associated diseases and disorders

PMCA1 has associations with conditions impacting calcium regulation such as hypertension and cardiovascular diseases. Dysfunctional regulation of PMCA1 can lead to abnormal calcium homeostasis contributing to such disorders. Furthermore PMCA1's interaction with proteins like the sodium-calcium exchanger (NCX) suggests a complex regulatory network that when disrupted may trigger or exacerbate disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
Lanes 1-4: Merged signal (red and green). Green - ab190355 observed at 180-245 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab190355 Anti-PMCA1 antibody [EPR12029] was shown to specifically react with PMCA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human ATP2B1 (PMCA1) knockout HeLa cell line ab265561 (knockout cell lysate Human ATP2B1 (PMCA1) knockout HeLa cell lysate ab257365) was used. Wild-type and PMCA1 knockout samples were subjected to SDS-PAGE. ab190355 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ATP2B1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ATP2B1 (PMCA1) knockout HeLa cell line (Human ATP2B1 (PMCA1) knockout HeLa cell line ab265561)
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: THP-1 cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 139 kDa
Observed band size: 180-245 kDa
Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
All lanes: Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution
Lane 1: U87-MG cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa
Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
All lanes: Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: Neuro-2a cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labelling PMCA1 with ab190355 at 1/2000 dilution, followed by ready-to-use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at dilution 1/1. Counterstained with Hematoxylin.
Performed heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining mouse stomach.
The section was incubated with ab190355 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
ab190355 was shown to react with ATP2B1 in wild-type HAP1 cells in Western blot with loss of signal observed in a ATP2B1 knockout cell line. Wild-type HAP1 and ATP2B1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab190355 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/1000 dilution
Lane 1: Wild-type HAP1 lysate at 30 µg
Lane 2: ATP2B1 knock-out HAP1 lysate at 30 µg
Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lanes 1, 2: 7 seconds

Lanes 3, 4, 5, 6: 3 seconds
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 is used as a Loading Control at dilution 1/1000000.
All lanes: Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/5000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, boiled at 20 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, unboiled at 20 µg
Lane 3: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate, boiled at 20 µg
Lane 4: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate, unboiled at 20 µg
Lane 5: C6 (Rat glial tumor glial cell) whole cell lysate, boiled at 20 µg
Lane 6: C6 (Rat glial tumor glial cell) whole cell lysate, unboiled at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labelling PMCA1 with ab190355 at 1/2000 dilution, followed by ready-to-use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at dilution 1/1. Counterstained with Hematoxylin.
Performed heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining mouse lung cancer.
The section was incubated with ab190355 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue labelling PMCA1 with ab190355 at 1/2000 dilution, followed by ready-to-use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at dilution 1/1. Counterstained with Hematoxylin.
Performed heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining mouse lung cancer.
The section was incubated with ab190355 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Immunoprecipitation - Anti-PMCA1 antibody [EPR12029] (ab190355)
Immunoprecipitation of ATP2B1 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 2.0 µg of ab190355 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Immunoprecipitation - Anti-PMCA1 antibody [EPR12029] (ab190355) at 2 µg
All lanes: HAP1 cells