Rabbit Recombinant Monoclonal Calcium Pump PMCA2 ATPase antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435220.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435216.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151)")
![Immunocytochemistry/ Immunofluorescence - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunocytochemistry-immunofluorescence-img435186.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151)")
![Immunocytochemistry/ Immunofluorescence - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunocytochemistry-immunofluorescence-img435187.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151)")
![Western blot - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--western-blot-img435173.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (AB319151)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | IP | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Expected | Not recommended |
| Mouse | Tested | Tested | Tested | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
ATP-driven Ca(2+) ion pump involved in the maintenance of basal intracellular Ca(2+) levels in specialized cells of cerebellar circuit and vestibular and cochlear systems (PubMed:17234811, PubMed:9668038). Uses ATP as an energy source to transport cytosolic Ca(2+) ions across the plasma membrane to the extracellular compartment. Has fast activation and Ca(2+) clearance rate suited to control fast neuronal Ca(2+) dynamics (PubMed:17409239, PubMed:20083513). At parallel fiber to Purkinje neuron synapse, mediates presynaptic Ca(2+) efflux in response to climbing fiber-induced Ca(2+) rise. Provides for fast return of Ca(2+) concentrations back to their resting levels, ultimately contributing to long-term depression induction and motor learning (PubMed:17409239, PubMed:20083513). Plays an essential role in hearing and balance. In cochlear hair cells, shuttles Ca(2+) ions from stereocilia to the endolymph and dissipates Ca(2+) transients generated by the opening of the mechanoelectrical transduction channels. Regulates Ca(2+) levels in the vestibular system, where it contributes to the formation of otoconia (By similarity) (PubMed:9668038). Regulates Ca(2+) signaling through dissipation of Ca(2+) transients generated by store-operated channels (By similarity). In lactating mammary gland, allows for the high content of Ca(2+) ions in the milk (PubMed:15302868).
Alternative names
Pmca2, Plasma membrane calcium-transporting ATPase 2, PMCA2, Plasma membrane calcium ATPase isoform 2, Plasma membrane calcium pump isoform 2
Recommended products
Rabbit Recombinant Monoclonal Calcium Pump PMCA2 ATPase antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28923-56
- Purification technique
- Affinity purification Protein A
- Specificity
Not suitable for human IHC-P and rat ICC. This antibody weakly cross-react with PMCA1, PMCA3 and PMCA4 overexpression lysates.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab319151 is the carrier-free version of Anti-PMCA2 antibody [EPR28923-56] ab319150.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Calcium Pump PMCA2 ATPase also known as PMCA2 or Ca-ATPase pump plays an important role in the active transport of calcium ions from the cytoplasm across the plasma membrane. It functions by hydrolyzing ATP to provide the energy needed for this process. PMCA2 belongs to the family of P-type ATPases and has a molecular mass of approximately 135 kDa. This pump is prominently expressed in the brain and inner ear particularly in the cerebellum and cochlea highlighting its importance in neuronal and auditory functions.
- Biological function summary
PMCA2 regulates intracellular calcium concentration which is critical for cellular signaling and homeostasis. It does not form part of a larger complex but works independently to balance calcium levels. In neuronal cells efficient calcium clearance is necessary for synaptic activity and PMCA2 helps in maintaining the rapid calcium ion flux during neuronal firing. The presence of PMCA2 in auditory hair cells signifies its role in cochlear function and hearing sensitivity by aiding the processing of sound signals through calcium modulation.
- Pathways
PMCA2 integrates into calcium signaling pathways and interacts with calmodulin which enhances its activity. Its function in the calmodulin-calcium signaling pathway is essential for processes such as neurotransmitter release and muscle contraction. In association with proteins like calmodulin PMCA2 ensures precise calcium dynamics safeguarding against cytotoxicity triggered by excessive calcium accumulation. The coordination with calmodulin formulates a pivotal connection within various signaling cascades.
- Associated diseases and disorders
PMCA2 mutations or dysfunctions have associations with neurological disorders and hearing impairments. Research links PMCA2 dysregulation to conditions such as spinocerebellar ataxia and hereditary hearing loss. In these scenarios PMCA2 interacts with molecular components that when impaired disrupt the normal calcium homeostasis. Such interruptions can lead to neurodegeneration or compromised auditory function underlining the significance of PMCA2 in maintaining health and preventing disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435220.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)
This data was developed using Anti-PMCA2 antibody [EPR28923-56] ab319150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PMCA2 with Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat lung.
The section was incubated with Anti-PMCA2 antibody [EPR28923-56] ab319150 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435216.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)
This data was developed using Anti-PMCA2 antibody [EPR28923-56] ab319150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PMCA2 with Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum (PMID: 19735545).
The section was incubated with Anti-PMCA2 antibody [EPR28923-56] ab319150 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND®
RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunocytochemistry/ Immunofluorescence - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunocytochemistry-immunofluorescence-img435186.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
Immunocytochemistry/ Immunofluorescence - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)
This data was developed using Anti-PMCA2 antibody [EPR28923-56] ab319150, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling PMCA2 with Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/50 (10.36 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in part of the mouse primary neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
-ve control 2: Anti-MAP2 antibody [HM-2] ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunocytochemistry-immunofluorescence-img435187.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
Immunocytochemistry/ Immunofluorescence - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)
This data was developed using Anti-PMCA2 antibody [EPR28923-56] ab319150, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling PMCA2 with Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/50 (10.36 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Negative control: Confocal image showing no staining in mouse splenocytes. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Western blot - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--western-blot-img435173.jpg "Western blot - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
Western blot - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)
This data was developed using Anti-PMCA2 antibody [EPR28923-56] ab319150, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: lung, testis, spleen (PMID: 1531651)
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-PMCA2 antibody [EPR28923-56] (Anti-PMCA2 antibody [EPR28923-56] ab319150) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse retina tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Lane 4: Mouse testis tissue lysate at 20 µg
Lane 5: Mouse spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa, 36 kDa
Exposure time: 37s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435218.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)
This data was developed using Anti-PMCA2 antibody [EPR28923-56] ab319150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling PMCA2 with Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum.
The section was incubated with Anti-PMCA2 antibody [EPR28923-56] ab319150 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435219.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)
This data was developed using Anti-PMCA2 antibody [EPR28923-56] ab319150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PMCA2 with Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse lung.
The section was incubated with Anti-PMCA2 antibody [EPR28923-56] ab319150 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435217.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)
This data was developed using Anti-PMCA2 antibody [EPR28923-56] ab319150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spinal cord tissue labeling PMCA2 with Anti-PMCA2 antibody [EPR28923-56] ab319150 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spinal cord (PMID: 31703709).
The section was incubated with Anti-PMCA2 antibody [EPR28923-56] ab319150 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND®
RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435218.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435219.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151), expandable thumbnail](https://content.abcam.com/products/images/pmca2-antibody-epr28923-56-bsa-and-azide-free-ab319151--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435217.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free (ab319151)")
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