AB154569

Anti-PME-1 antibody

Name: Anti-PME-1 antibody (ab154569) | Abcam
Brand: Abcam Limited
SKU: AB154569

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(1 Publication)

Rabbit Polyclonal PME-1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PPME1 aa 1-300.

View Alternative Names

PME1, PP2593, PRO0750, PPME1, Protein phosphatase methylesterase 1, PME-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PME-1 antibody (AB154569)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PME-1 antibody (AB154569)

Immunohistochemical analysis of paraffin-embedded Human Hepatoma tissue labeling PME-1 with ab154569 at 1/500 dilution.

Unknown

Western blot - Anti-PME-1 antibody (AB154569)

10% SDS PAGE

All lanes:

Western blot - Anti-PME-1 antibody (ab154569) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Predicted band size: 42 kDa

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Western blot - Anti-PME-1 antibody (AB154569)

10% SDS PAGE

All lanes:

Western blot - Anti-PME-1 antibody (ab154569) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 30 µg

Lane 2:

JC whole cell lysate at 30 µg

Predicted band size: 37 kDa,42 kDa,57 kDa,69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PPME1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9Y570

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase methylesterase 1 (PME-1) also known as protein phosphatase 2A methylesterase is an enzyme that regulates protein function by demethylation. PME-1 has a mass of approximately 42 kDa. It primarily resides in the cytoplasm and nucleus of cells with high expression levels in brain tissue. Its function involves the removal of methyl groups from the carboxyl-terminal leucine residue of the catalytic subunit of protein phosphatase 2A (PP2A). This action regulates the activity states of PP2A a major serine/threonine phosphatase.

Biological function summary

PME-1 influences the stability and activity of PP2A a core component of cellular signal transduction pathways. PME-1 forms part of the regulatory complexes of PP2A modulating its methylation state and associated functional properties. This regulation by PME-1 impacts cell cycle control apoptosis and other critical cellular processes. Additionally PME-1 activity affects signaling pathways by altering PP2A's interaction with various substrates and regulatory proteins.

Pathways

The role of PME-1 in cellular mechanisms links it to the Wnt signaling pathway and MAP kinase signaling cascade. PME-1 catalyzes demethylation reactions that impact PP2A's ability to associate with specific regulatory subunits. Through the Wnt pathway PME-1 affects cellular processes such as proliferation and differentiation by regulating beta-catenin activity. In relation to the MAP kinase pathway PME-1 influences cell stress response and growth factor signaling interacting with proteins like ERK and p38 MAP kinase.

PME-1 has associations with cancer and neurodegenerative conditions. In cancer altered PME-1 expression or activity can lead to uncontrolled cell growth and survival acting through PP2A-related pathways. In neurodegenerative diseases such as Alzheimer's PME-1 may affect tau protein phosphorylation by modulating PP2A activity potentially contributing to pathogenesis. As PP2A regulates tau phosphorylation disruptions in its demethylation by PME-1 has been correlated with disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.

See full target information PPME1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science signaling 8:ra72 PubMed26198358

2015

Protein phosphatase 2A regulatory subunit B56α limits phosphatase activity in the heart.

Applications

Unspecified application

Species

Unspecified reactive species

Sean C Little,Jerry Curran,Michael A Makara,Crystal F Kline,Hsiang-Ting Ho,Zhaobin Xu,Xiangqiong Wu,Iuliia Polina,Hassan Musa,Allison M Meadows,Cynthia A Carnes,Brandon J Biesiadecki,Jonathan P Davis,Noah Weisleder,Sandor Györke,Xander H Wehrens,Thomas J Hund,Peter J Mohler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PME-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PME-1 antibody (AB154569)

Western blot - Anti-PME-1 antibody (AB154569)

Western blot - Anti-PME-1 antibody (AB154569)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise