AB199531

Anti-PMF-1 antibody [EPR17298]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PMF-1 antibody. Suitable for WB, IP and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

View Alternative Names

Polyamine-modulated factor 1, PMF-1, PMF1

4 Images

Supplier Data

Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] (AB199531)

PMF-1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179466 at 1/80 (or 5 μg). Western blot was performed using ab199531 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HeLa whole cell extract at 10ug (input)

Lane 2 : HeLa whole cell extract PMF-1 immunoprecipitate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199531 in HeLa whole cell extract immunoprecipitation.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] (ab199531)

Predicted band size: 23 kDa

Observed band size: 26 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-PMF-1 antibody [EPR17298] (AB199531)

Blocking and diluting buffer was 5% NFDM /TBST.

All lanes:

Western blot - Anti-PMF-1 antibody [EPR17298] (ab199531) at 1/5000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-PMF-1 antibody [EPR17298] (AB199531)

Blocking and diluting buffer was 5% NFDM /TBST.

All lanes:

Western blot - Anti-PMF-1 antibody [EPR17298] (ab199531) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-PMF-1 antibody [EPR17298] (AB199531)

Blocking and diluting buffer was 5% NFDM /TBST.

All lanes:

Western blot - Anti-PMF-1 antibody [EPR17298] (ab199531) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

true

Exposure time: 30s

Carrier free

Anti-PMF-1 antibody [EPR17298] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17298

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/80", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/80", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/80", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PMF-1 also recognized as Polyamine-modulated factor 1 is a protein with a molecular mass of around 63 kDa. It plays a mechanical role in transcriptional regulation by interacting with other proteins and DNA sequences. PMF-1 is expressed mainly in the nucleus of cells across various tissues. It partners with other transcription factors modulating gene expression essential for cellular growth and differentiation. This interaction underlines its importance in transcriptional control.

Biological function summary

PMF-1 engages in modifying transcriptional responses by forming part of the TCF/LEF transcription factor complex. This complex influences the expression of genes involved in cellular proliferation and differentiation. PMF-1 functions by binding to specific DNA sequences enhancing or repressing the transcription of target genes. Through these actions it serves as a regulatory hub for controlling numerous cellular responses including but not limited to cell cycle progression and apoptosis.

Pathways

PMF-1 interacts significantly within the Wnt signaling pathway. This pathway is pivotal for regulating cellular processes related to development and growth. PMF-1 associates with proteins such as β-catenin facilitating the transcriptional activation of Wnt target genes. Through this pathway PMF-1 helps transmit signals that dictate cellular fate decisions illustrating its importance in maintaining physiological balance.

PMF-1 has notable implications in cancer and neurodegenerative conditions. In cancer its association with the Wnt signaling pathway implies roles in cell proliferation and tumor progression. The interaction with β-catenin further solidifies its connection to oncogenic processes. In neurodegenerative disorders altered PMF-1 activity can influence neuronal growth and survival implicating it in pathologies like Alzheimer's disease through dysregulated transcriptional responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.

See full target information PMF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eade5041 PubMed37043568

2023

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjun Tong,Sitong Zhang,Qiqi Liu,Chenyuan Huang,Haining Hao,Michelle Siying Tan,Xiaodong Yu,Charles Kang Liang Lou,Rong Huang,Zhe Zhang,Tongjie Liu,Pimin Gong,Cheng Han Ng,Mark Muthiah,Giorgia Pastorin,Matthias Gerhard Wacker,Xiaoyuan Chen,Gert Storm,Cheun Neng Lee,Lanwei Zhang,Huaxi Yi,Jiong-Wei Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PMF-1 antibody [EPR17298]

Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] (AB199531)

Western blot - Anti-PMF-1 antibody [EPR17298] (AB199531)

Western blot - Anti-PMF-1 antibody [EPR17298] (AB199531)

Western blot - Anti-PMF-1 antibody [EPR17298] (AB199531)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise