Anti-PMF-1 antibody [EPR17298] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280)

This data was developed using ab199531, the same antibody clone in a different buffer formulation.

PMF-1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179466 at 1/80 (or 5 μg). Western blot was performed using ab199531 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HeLa whole cell extract at 10ug (input)

Lane 2 : HeLa whole cell extract PMF-1 immunoprecipitate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199531 in HeLa whole cell extract immunoprecipitation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] (<a href='/en-us/products/primary-antibodies/pmf-1-antibody-epr17298-ab199531'>ab199531</a>)

Predicted band size: 23 kDa

Observed band size: 26 kDa

true

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280)

This data was developed using ab199531, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PMF-1 antibody [EPR17298] (<a href='/en-us/products/primary-antibodies/pmf-1-antibody-epr17298-ab199531'>ab199531</a>) at 1/5000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280)

This data was developed using ab199531, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PMF-1 antibody [EPR17298] (<a href='/en-us/products/primary-antibodies/pmf-1-antibody-epr17298-ab199531'>ab199531</a>) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

true

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280)

This data was developed using ab199531, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PMF-1 antibody [EPR17298] (<a href='/en-us/products/primary-antibodies/pmf-1-antibody-epr17298-ab199531'>ab199531</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

true

Exposure time: 30s