Rabbit Recombinant Monoclonal PMF-1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280), expandable thumbnail](https://content.abcam.com/products/images/pmf-1-antibody-epr17298-bsa-and-azide-free-ab251280--western-blot-img107760.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280)")
![Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280), expandable thumbnail](https://content.abcam.com/products/images/pmf-1-antibody-epr17298-bsa-and-azide-free-ab251280--western-blot-img62281.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280)")
![Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280), expandable thumbnail](https://content.abcam.com/products/images/pmf-1-antibody-epr17298-bsa-and-azide-free-ab251280--immunoprecipitation-img685.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280)")
![Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280), expandable thumbnail](https://content.abcam.com/products/images/pmf-1-antibody-epr17298-bsa-and-azide-free-ab251280--western-blot-img102461.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (AB251280)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
Alternative names
Polyamine-modulated factor 1, PMF-1, PMF1
Recommended products
Rabbit Recombinant Monoclonal PMF-1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17298
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251280 is the carrier-free version of Anti-PMF-1 antibody [EPR17298] ab199531.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PMF-1 also recognized as Polyamine-modulated factor 1 is a protein with a molecular mass of around 63 kDa. It plays a mechanical role in transcriptional regulation by interacting with other proteins and DNA sequences. PMF-1 is expressed mainly in the nucleus of cells across various tissues. It partners with other transcription factors modulating gene expression essential for cellular growth and differentiation. This interaction underlines its importance in transcriptional control.
- Biological function summary
PMF-1 engages in modifying transcriptional responses by forming part of the TCF/LEF transcription factor complex. This complex influences the expression of genes involved in cellular proliferation and differentiation. PMF-1 functions by binding to specific DNA sequences enhancing or repressing the transcription of target genes. Through these actions it serves as a regulatory hub for controlling numerous cellular responses including but not limited to cell cycle progression and apoptosis.
- Pathways
PMF-1 interacts significantly within the Wnt signaling pathway. This pathway is pivotal for regulating cellular processes related to development and growth. PMF-1 associates with proteins such as β-catenin facilitating the transcriptional activation of Wnt target genes. Through this pathway PMF-1 helps transmit signals that dictate cellular fate decisions illustrating its importance in maintaining physiological balance.
- Associated diseases and disorders
PMF-1 has notable implications in cancer and neurodegenerative conditions. In cancer its association with the Wnt signaling pathway implies roles in cell proliferation and tumor progression. The interaction with β-catenin further solidifies its connection to oncogenic processes. In neurodegenerative disorders altered PMF-1 activity can influence neuronal growth and survival implicating it in pathologies like Alzheimer's disease through dysregulated transcriptional responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280), expandable thumbnail](https://content.abcam.com/products/images/pmf-1-antibody-epr17298-bsa-and-azide-free-ab251280--western-blot-img107760.jpg "Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)")
Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
This data was developed using Anti-PMF-1 antibody [EPR17298] ab199531, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PMF-1 antibody [EPR17298] (Anti-PMF-1 antibody [EPR17298] ab199531) at 1/1000 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg
Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Lane 3: HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg
Lane 4: HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 26 kDa
Exposure time: 30s
![Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280), expandable thumbnail](https://content.abcam.com/products/images/pmf-1-antibody-epr17298-bsa-and-azide-free-ab251280--western-blot-img62281.jpg "Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)")
Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
This data was developed using Anti-PMF-1 antibody [EPR17298] ab199531, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PMF-1 antibody [EPR17298] (Anti-PMF-1 antibody [EPR17298] ab199531) at 1/5000 dilution
Lane 1: Human fetal brain tissue lysate at 10 µg
Lane 2: Human fetal kidney tissue lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 26 kDa
Exposure time: 3min
![Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280), expandable thumbnail](https://content.abcam.com/products/images/pmf-1-antibody-epr17298-bsa-and-azide-free-ab251280--immunoprecipitation-img685.jpg "Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)")
Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
This data was developed using Anti-PMF-1 antibody [EPR17298] ab199531, the same antibody clone in a different buffer formulation.
PMF-1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with Anti-PML Protein antibody [EPR16792] ab179466 at 1/80 (or 5 μg). Western blot was performed using Anti-PMF-1 antibody [EPR17298] ab199531 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell extract at 10ug (input)
Lane 2: HeLa whole cell extract PMF-1 immunoprecipitate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PMF-1 antibody [EPR17298] ab199531 in HeLa whole cell extract immunoprecipitation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] (Anti-PMF-1 antibody [EPR17298] ab199531)
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 26 kDa
Exposure time: 30s
![Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280), expandable thumbnail](https://content.abcam.com/products/images/pmf-1-antibody-epr17298-bsa-and-azide-free-ab251280--western-blot-img102461.jpg "Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)")
Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
This data was developed using Anti-PMF-1 antibody [EPR17298] ab199531, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PMF-1 antibody [EPR17298] (Anti-PMF-1 antibody [EPR17298] ab199531) at 1/1000 dilution
Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 10 µg
Lane 2: Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 3: NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 26 kDa
Exposure time: 30s
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