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Rabbit Recombinant Monoclonal PMM1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-PMM1 antibody [EPR17844] (AB202058), expandable thumbnail
  • Western blot - Anti-PMM1 antibody [EPR17844] (AB202058), expandable thumbnail
  • Western blot - Anti-PMM1 antibody [EPR17844] (AB202058), expandable thumbnail
  • Western blot - Anti-PMM1 antibody [EPR17844] (AB202058), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (AB202058), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.

Alternative names

PMMH22, PMM1, Phosphomannomutase 1, PMM 1, PMMH-22

Recommended products

Rabbit Recombinant Monoclonal PMM1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17844
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein Phosphomannomutase 1 (PMM1) plays an essential role in carbohydrate metabolism by catalyzing the interconversion of mannose-6-phosphate and mannose-1-phosphate. The protein is also known as PMM-1 and has a molecular weight of approximately 28 kDa. PMM1 is expressed in various tissues including the liver kidney and muscle indicating its wide physiological relevance. Its enzymatic function is important for producing GDP-mannose which is necessary for glycosylation processes.

Biological function summary

PMM1 facilitates the conversion of mannose phosphates which is important in protein glycosylation pathways particularly in the synthesis of N-linked glycoproteins. PMM1 operates as a monomer and it does not form part of any larger enzymatic complex. Its activity ensures a proper supply of mannose-1-phosphate required for synthesizing GDP-mannose a donor substrate for glycosylation reactions that is critical in the production of structural molecules and signaling pathways.

Pathways

PMM1 plays a significant role in the biosynthesis of GDP-mannose and impacts the N-glycosylation pathway. PMM1's enzymatic activities integrate closely with glycosylation pathways interfacing with proteins involved in glycan assembly and modification. It interacts with other metabolic pathways that involve mannose and cooperatively influences the cellular processes for protein targeting and function through glycosylation alongside proteins like phosphomannose isomerase (PMI).

Associated diseases and disorders

PMM1 mutations and dysregulation show potential links to congenital disorders of glycosylation (CDG). These disorders arise from improper glycosylation affecting a variety of bodily systems. PMM1's function and interaction with other glycosylating proteins such as PMM2 is critical in addressing the potential glycosylation defects that contribute to these rare genetic disorders. Although detailed studies are ongoing PMM1 remains a vital focus in understanding the molecular basis of these diseases and potentially developing therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-PMM1 antibody [EPR17844] (ab202058), expandable thumbnail

    Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PMM1 antibody [EPR17844] (ab202058) at 1/5000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

    Lane 3: 293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 30 kDa

    Exposure time: 1min

  • Western blot - Anti-PMM1 antibody [EPR17844] (ab202058), expandable thumbnail

    Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PMM1 antibody [EPR17844] (ab202058) at 1/2000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 30 kDa

    Exposure time: 3min

  • Western blot - Anti-PMM1 antibody [EPR17844] (ab202058), expandable thumbnail

    Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PMM1 antibody [EPR17844] (ab202058) at 1/2000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Rat brain tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 30 kDa

    Exposure time: 30s

  • Western blot - Anti-PMM1 antibody [EPR17844] (ab202058), expandable thumbnail

    Western blot - Anti-PMM1 antibody [EPR17844] (ab202058)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PMM1 antibody [EPR17844] (ab202058) at 1/5000 dilution

    Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 30 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PMM1 with ab202058 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling PMM1 with ab202058 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse cerebral cortex is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] (ab202058)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PMM1 with ab202058 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat pancreas is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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