AB229996

Anti-PMM2 antibody

Name: Anti-PMM2 antibody (ab229996) | Abcam
Brand: Abcam Limited
SKU: AB229996

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(3 Publications)

Rabbit Polyclonal PMM2 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PMM2.

View Alternative Names

Phosphomannomutase 2, PMM 2, PMM2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

Paraffin-embedded human thyroid tissue stained for PMM2 using ab229996 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

Paraffin-embedded human gastric cancer tissue stained for PMM2 using ab229996 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-PMM2 antibody (AB229996)

PMM2 was immunoprecipitated from 500 μg HeLa (Human breast adenocarcinoma cell line) whole cell lysate using 4 μg of ab229996.

Lane 1 : Rabbit monoclonal IgG (1 μg) IP in HeLa whole cell lysate.

Lane 2 : ab229996 IP in HeLa whole cell lysate.

Lane 3 : HeLa whole cell lysate 20 μg (Input).

For western blotting an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at 1/50000 dilution.

All lanes:

Immunoprecipitation - Anti-PMM2 antibody (ab229996)

Predicted band size: 28 kDa

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Supplier Data

Western blot - Anti-PMM2 antibody (AB229996)

All lanes:

Western blot - Anti-PMM2 antibody (ab229996) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lane 4:

K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Lane 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 6:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PMM2.

O15305

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphomannomutase 2 (PMM2) also known as PMM 22 or PMM II is an enzyme that plays a role in the conversion of mannose-6-phosphate to mannose-1-phosphate. PMM2 is about 29 kDa in size and exists predominantly in the cytoplasm of cells. It shows widespread expression but higher levels are found in the liver brain and muscle tissues. PMM2 activity is important in carbohydrate metabolism making it an enzyme of interest especially in the context of glycobiology and metabolic research.

Biological function summary

PMM2 catalyzes an important step in the biosynthesis of GDP-mannose which is necessary for glycan production. These glycans are essential in the process of glycosylation a post-translational modification of proteins and lipids. PMM2 acts independently and does not form part of a larger protein complex. Through its role in glycan synthesis PMM2 impacts cell signaling protein folding and molecular interactions.

Pathways

PMM2 is involved in the N-glycosylation pathway and the GDP-mannose biosynthesis pathway. It collaborates closely with proteins such as PMM1 as both contribute to mannose metabolism and glycan assembly. PMM2 is significant in these pathways as it impacts not only the formation of sugar nucleotides but also their integration into larger biomolecules.

PMM2 dysfunction is directly linked to Congenital Disorders of Glycosylation Type Ia (CDG-Ia). This genetic disorder results from mutations in the PMM2 gene impairing glycosylation processes and resulting in multisystemic clinical manifestations. The protein PMM1 while not directly associated with the disorder serves complementary functions in mannose phosphate isomerization and may modulate the impact of PMM2 deficiencies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

See full target information PMM2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular endocrinology 584:112160 PubMed38266771

2024

PMM2 controls ERα levels and cell proliferation in ESR1 Y537S variant expressing breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Cipolletti,Filippo Acconcia

Frontiers in veterinary science 7:570593 PubMed33240950

2020

Effects of Probiotics BaSC06 on Intestinal Digestion and Absorption, Antioxidant Capacity, Microbiota Composition, and Macrophage Polarization in Pigs for Fattening.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefang Cao,Li Tang,Zihan Zeng,Baikui Wang,Yuanhao Zhou,Qi Wang,Peng Zou,Weifen Li

Journal of assisted reproduction and genetics 37:443-450 PubMed31902100

2020

Novel PMM2 missense mutation in a Chinese family with non-syndromic premature ovarian insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Tianliu Peng,Chao Lv,Hangjing Tan,Jiafeng Huang,Hailun He,Yan Wang,Minghua Zeng,Dajing Yi,Jie Li,Hongwen Deng,Xiaobo Shi,Hongmei Xiao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PMM2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

Immunoprecipitation - Anti-PMM2 antibody (AB229996)

Western blot - Anti-PMM2 antibody (AB229996)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

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