AB90782

Anti-PMP22 antibody [CF1]

Name: Anti-PMP22 antibody [CF1] (ab90782) | Abcam
Brand: Abcam Limited
SKU: AB90782
Price: 535 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal PMP22 antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PMP22 aa 100-150.

View Alternative Names

GAS3, PMP22, Peripheral myelin protein 22, PMP-22, Growth arrest-specific protein 3, GAS-3

2 Images

Flow Cytometry (Intracellular) - Anti-PMP22 antibody [CF1] (AB90782)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PMP22 antibody [CF1] (AB90782)

Overlay histogram showing SH-SY5Y cells stained with ab90782 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90782, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [CF1] (AB90782)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [CF1] (AB90782)

Formalin fixed, paraffin embedded human nerve tissue labelled with ab90782 at 1 μg/ml. Positive staining of peripheral nerve myelin sheaths can be observed. Image = 20x magnification.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CF1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human PMP22 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q01453

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg/cells for 6 µg/cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification notes

0.2 µm filtered antibody solution

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'PMP22 protein' also known as Peripheral Myelin Protein 22 is an essential component of the myelin sheath in the peripheral nervous system. It has a molecular weight of approximately 22 kDa. The protein is highly expressed in Schwann cells which are responsible for forming myelin around peripheral nerves. PMP22 contains four transmembrane domains playing a role in its structural function in the membrane.

Biological function summary

The 'PMP22' serves an important role in maintaining the integrity and functionality of the myelin sheath contributing to efficient nerve conduction. It is not typically part of a complex but interacts with other myelin proteins to support myelin compaction and stability. The protein's presence affects the cell cycle and myelination process important for the normal functioning of the peripheral nervous system.

Pathways

'PMP22 protein' plays roles in the Schwann cell differentiation and myelination pathways. It maintains correct peripheral nerve structure and function with connections to proteins such as MPZ (Myelin Protein Zero) which are also integral for proper nerve insulation. There are specific pathways where PMP22 downregulation or overexpression impacts the normal myelination cycle significant for peripheral nerve health.

'PMP 22' mutations or overexpression relate to Charcot-Marie-Tooth disease type 1A and Hereditary Neuropathy with Liability to Pressure Palsies (HNPP). These conditions arise from gene dosage changes affecting nerve function where altered PMP22 protein levels lead to defective myelin formation. Interaction between PMP22 and proteins like connexin 32 shows further ties to demyelinating neuropathies emphasizing the gene-protein network involved in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Might be involved in growth regulation, and in myelinization in the peripheral nervous system.

See full target information PMP22

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7064 PubMed39152100

2024

De novo design of miniprotein antagonists of cytokine storm inducers.

Applications

Unspecified application

Species

Unspecified reactive species

Buwei Huang,Brian Coventry,Marta T Borowska,Dimitrios C Arhontoulis,Marc Exposit,Mohamad Abedi,Kevin M Jude,Samer F Halabiya,Aza Allen,Cami Cordray,Inna Goreshnik,Maggie Ahlrichs,Sidney Chan,Hillary Tunggal,Michelle DeWitt,Nathaniel Hyams,Lauren Carter,Lance Stewart,Deborah H Fuller,Ying Mei,K Christopher Garcia,David Baker

The Journal of clinical investigation 132: PubMed35579942

2022

A translatable RNAi-driven gene therapy silences PMP22/Pmp22 genes and improves neuropathy in CMT1A mice.

Applications

Species

Mouse

Marina Stavrou,Alexia Kagiava,Sarah G Choudury,Matthew J Jennings,Lindsay M Wallace,Allison M Fowler,Amanda Heslegrave,Jan Richter,Christina Tryfonos,Christina Christodoulou,Henrik Zetterberg,Rita Horvath,Scott Q Harper,Kleopas A Kleopa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PMP22 antibody [CF1]

Flow Cytometry (Intracellular) - Anti-PMP22 antibody [CF1] (AB90782)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [CF1] (AB90782)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise