Rabbit Recombinant Monoclonal PMP22 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (AB303559), expandable thumbnail](https://content.abcam.com/products/images/pmp22-antibody-epr26310-118-5-bsa-and-azide-free-ab303559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img402247.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (AB303559)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (AB303559), expandable thumbnail](https://content.abcam.com/products/images/pmp22-antibody-epr26310-118-5-bsa-and-azide-free-ab303559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img402246.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (AB303559)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (AB303559), expandable thumbnail](https://content.abcam.com/products/images/pmp22-antibody-epr26310-118-5-bsa-and-azide-free-ab303559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img402245.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (AB303559)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | Flow Cyt | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Might be involved in growth regulation, and in myelinization in the peripheral nervous system.
Alternative names
Gas-3, Gas3, Pmp-22, Pmp22, Peripheral myelin protein 22, PMP-22, Growth arrest-specific protein 3, GAS-3
Recommended products
Rabbit Recombinant Monoclonal PMP22 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26310-118-5
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 'PMP22 protein' also known as Peripheral Myelin Protein 22 is an essential component of the myelin sheath in the peripheral nervous system. It has a molecular weight of approximately 22 kDa. The protein is highly expressed in Schwann cells which are responsible for forming myelin around peripheral nerves. PMP22 contains four transmembrane domains playing a role in its structural function in the membrane.
- Biological function summary
The 'PMP22' serves an important role in maintaining the integrity and functionality of the myelin sheath contributing to efficient nerve conduction. It is not typically part of a complex but interacts with other myelin proteins to support myelin compaction and stability. The protein's presence affects the cell cycle and myelination process important for the normal functioning of the peripheral nervous system.
- Pathways
'PMP22 protein' plays roles in the Schwann cell differentiation and myelination pathways. It maintains correct peripheral nerve structure and function with connections to proteins such as MPZ (Myelin Protein Zero) which are also integral for proper nerve insulation. There are specific pathways where PMP22 downregulation or overexpression impacts the normal myelination cycle significant for peripheral nerve health.
- Associated diseases and disorders
'PMP 22' mutations or overexpression relate to Charcot-Marie-Tooth disease type 1A and Hereditary Neuropathy with Liability to Pressure Palsies (HNPP). These conditions arise from gene dosage changes affecting nerve function where altered PMP22 protein levels lead to defective myelin formation. Interaction between PMP22 and proteins like connexin 32 shows further ties to demyelinating neuropathies emphasizing the gene-protein network involved in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (ab303559), expandable thumbnail](https://content.abcam.com/products/images/pmp22-antibody-epr26310-118-5-bsa-and-azide-free-ab303559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img402247.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (ab303559)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (ab303559)
This data was developed using Anti-PMP22 antibody [EPR26310-118-5] ab303558, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling PMP22 with Anti-PMP22 antibody [EPR26310-118-5] ab303558 at 1/2000 (0.27 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: No staining on mouse kidney. The section was incubated with Anti-PMP22 antibody [EPR26310-118-5] ab303558 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (ab303559), expandable thumbnail](https://content.abcam.com/products/images/pmp22-antibody-epr26310-118-5-bsa-and-azide-free-ab303559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img402246.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (ab303559)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (ab303559)
This data was developed using Anti-PMP22 antibody [EPR26310-118-5] ab303558, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse sciatic nerve tissue labeling PMP22 with Anti-PMP22 antibody [EPR26310-118-5] ab303558 at 1/2000 (0.27 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on mouse sciatic nerve. The section was incubated with Anti-PMP22 antibody [EPR26310-118-5] ab303558 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (ab303559), expandable thumbnail](https://content.abcam.com/products/images/pmp22-antibody-epr26310-118-5-bsa-and-azide-free-ab303559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img402245.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (ab303559)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (ab303559)
This data was developed using Anti-PMP22 antibody [EPR26310-118-5] ab303558, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat sciatic nerve tissue labeling PMP22 with Anti-PMP22 antibody [EPR26310-118-5] ab303558 at 1/500 (1.08 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on rat sciatic nerve. The section was incubated with Anti-PMP22 antibody [EPR26310-118-5] ab303558 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com