Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (AB303559)

This data was developed using ab303558, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat sciatic nerve tissue labeling PMP22 with ab303558 at 1/500 (1.08 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on rat sciatic nerve. The section was incubated with ab303558 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (AB303559)

This data was developed using ab303558, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling PMP22 with ab303558 at 1/2000 (0.27 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining on mouse kidney. The section was incubated with ab303558 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (AB303559)

This data was developed using ab303558, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse sciatic nerve tissue labeling PMP22 with ab303558 at 1/2000 (0.27 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse sciatic nerve. The section was incubated with ab303558 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-PMP22 antibody [EPR26310-118-5] - BSA and Azide free (AB303559)

This data was developed using ab303558, the same antibody clone in a different buffer formulation. Blocking and dilution buffer and concentration : 5% NFDM/TBST.  The molecular weight observed is consistent with what has been described in the literature (PMID : 7649472). Negative controls : brain, kidney (PMID : 7649472) The blot of 4-6 was developed using a high sensitivity ECL substrate. The identity of the higher MW band in lane 4 is unknown. Exposure time : Lane 1-3 : 3minutes Lane 4-6 : 103 seconds

All lanes:

Western blot - Anti-PMP22 antibody [EPR26310-118-5] (<a href='/en-us/products/primary-antibodies/pmp22-antibody-epr26310-118-5-ab303558'>ab303558</a>) at 1/1000 dilution

Lane 1:

Mouse sciatic nerve tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Rat sciatic nerve tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

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