Anti-PMP70 antibody (ab3421) is a rabbit polyclonal antibody that is used to detect PMP70 in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 50 publications
- Trusted since 2003
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- Theranostics 14:56-742024Selective induction of Rab9-dependent alternative mitophagy using a synthetic derivative of isoquinoline alleviates mitochondrial dysfunction and cognitive deficits in Alzheimer's disease models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJee-Hyun Um,Dong Jin Shin,Se Myeong Choi,Alen Benhur Pravin Nathan,Young Yeon Kim,Da Ye Lee,Dae Jin Jeong,Dong Hyun Kim,Kyung Hwa Kim,Young Hye Kim,Jihoon Nah,Jeong-Hee Jeong,Eunhee Yoo,Hwa Kyoung Shin,Hwan Tae Park,Jihoon Jo,Jong Hyun Cho,Jeanho YunPubMed 38164158
- Nature communications 14:73482023An adaptive stress response that confers cellular resilience to decreased ubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiam C Hunt,Vishwajeeth Pagala,Anna Stephan,Boer Xie,Kiran Kodali,Kanisha Kavdia,Yong-Dong Wang,Abbas Shirinifard,Michelle Curley,Flavia A Graca,Yingxue Fu,Suresh Poudel,Yuxin Li,Xusheng Wang,Haiyan Tan,Junmin Peng,Fabio DemontisPubMed 37963875
- International journal of molecular sciences 24:2023Reciprocal Regulation of Peroxisome Biogenesis and Myogenic Factors Is Critical for Myogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuan-Che Wu,Wei-Cheng Chen,Wen-Po Hsiao,Kai-Fan Huang,Yi-Shiuan Liao,Huang-Bin Lin,Yi-Ju Wu,Chien-Han Kao,Shen-Liang ChenPubMed 37569637
- The EMBO journal 42:e1125422023Coronaviral ORF6 protein mediates inter-organelle contacts and modulates host cell lipid flux for virus production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengzhen Yue,Bing Hu,Jiajia Li,Ruifeng Chen,Zhen Yuan,Hurong Xiao,Haishuang Chang,Yaming Jiu,Kun Cai,Binbin DingPubMed 37218505
- BMC cancer 23:3352023SLC27A2 mediates FAO in colorectal cancer through nongenic crosstalk regulation of the PPARs pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Shang,Nina Ma,Juanjuan Che,Huihui Li,Jiexuan Hu,Haolin Sun,Bangwei CaoPubMed 37041476
- Communications biology 6:82023Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Landowski,Vijesh J Bhute,Samuel Grindel,Zachary Haugstad,Yeboah K Gyening,Madison Tytanic,Richard S Brush,Lucas J Moyer,David W Nelson,Christopher R Davis,Chi-Liang Eric Yen,Sakae Ikeda,Martin-Paul Agbaga,Akihiro IkedaPubMed 36599953
- Cells 11:2022Loss of Pex1 in Inner Ear Hair Cells Contributes to Cochlear Synaptopathy and Hearing Loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephanie A Mauriac,Thibault Peineau,Aamir Zuberi,Cathleen Lutz,Gwénaëlle S G GéléocPubMed 36552747
- PloS one 17:e02757252022Myoglobin regulates fatty acid trafficking and lipid metabolism in mammary epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Armbruster,Mostafa A Aboouf,Max Gassmann,Angela Egert,Hubert Schorle,Veit Hornung,Tobias Schmidt,Jonathan L Schmid-Burgk,Glen Kristiansen,Anne Bicker,Thomas Hankeln,Hao Zhu,Thomas A GorrPubMed 36223378
- Life science alliance 5:2022Novel <i>DNM1L</i> variants impair mitochondrial dynamics through divergent mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelsey A Nolden,John M Egner,Jack J Collier,Oliver M Russell,Charlotte L Alston,Megan C Harwig,Michael E Widlansky,Souphatta Sasorith,Inês A Barbosa,Andrew Gl Douglas,Julia Baptista,Mark Walker,Deirdre E Donnelly,Andrew A Morris,Hui Jeen Tan,Manju A Kurian,Kathleen Gorman,Santosh Mordekar,Charu Deshpande,Rajib Samanta,Robert McFarland,R Blake Hill,Robert W Taylor,Monika OláhováPubMed 35914810
- Cells 11:2022Peroxisome Metabolism Contributes to PIEZO2-Mediated Mechanical Allodynia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Gong,Fiza Laheji,Anna Berenson,April Qian,Sang-O Park,Rene Kok,Martin Selig,Ryan Hahn,Reza Sadjadi,Stephan Kemp,Florian EichlerPubMed 35681537
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P16970
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Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Tested | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected | Expected |
Flow Cyt
TestedTested
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes 70kDa band represents PMP 70 from peroxisome-enriched fractions from L cells. |
Species Rat | Dilution info 1/500 | Notes 70kDa band represents PMP 70 from peroxisome-enriched fractions from L cells. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-10 µg/mL | Notes - |
Species Human | Dilution info 1-10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that catalyzes the transport of long-chain fatty acids (LCFA)-CoA, dicarboxylic acids-CoA, long-branched-chain fatty acids-CoA and bile acids from the cytosol to the peroxisome lumen for beta-oxydation. Has fatty acyl-CoA thioesterase and ATPase activities (By similarity). Probably hydrolyzes fatty acyl-CoAs into free fatty acids prior to their ATP-dependent transport into peroxisomes (By similarity). Thus, play a role in regulation of LCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation (PubMed:10207018).
Alternative names
Pmp70, Pxmp1, ATP-binding cassette sub-family D member 3, 70 kDa peroxisomal membrane protein, PMP70
Recommended products
Anti-PMP70 antibody (ab3421) is a rabbit polyclonal antibody that is used to detect PMP70 in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 50 publications
- Trusted since 2003
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P16970
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-PMP70 antibody (ab3421) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.
Recommended positive controls
WB: Rat kidney and liver tissue lysate, A431, U-2 OS, HepG2 whole cell lysates. Mouse lung and liver tissues.ICC: A431 KO, A431, NIH/3T3, HMVEC, NS-1, P19, A549 whole cells.IHC-P: Human duodenum tissue;Flow Cyt: HepG2, 293T, and NIH-3T3 cells
Trusted by the scientific community
Anti-PMP70 (ab3421) was first used in a scientific publication in 2003 and has been cited over 50 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PMP70 also known as ABCD3 is a peroxisomal membrane protein with a molecular weight of approximately 70 kDa. It belongs to the ATP-binding cassette (ABC) transporter family and localizes on the peroxisomal membrane predominantly expressed in liver kidney and testes. PMP70 plays an important mechanical role in the transportation of very long-chain fatty acids (VLCFAs) across the peroxisomal membrane.
- Biological function summary
This protein operates as part of a heterodimeric complex with its related transporter protein ALDP (ABCD1). PMP70 facilitates the import of VLCFAs into peroxisomes which are then subjected to β-oxidation. By doing this PMP70 contributes significantly to the metabolism of lipids maintaining internal cellular homeostasis.
- Pathways
PMP70 integrates into the peroxisomal β-oxidation pathway alongside other proteins such as ALDP and ACSVL1. It ensures the proper flux of VLCFAs into peroxisomes which is essential for their breakdown and energy production. The protein also interacts with metabolic pathways linked to lipid biosynthesis and degradation illustrating its multifaceted role in cellular lipid management.
- Associated diseases and disorders
PMP70's dysfunction relates to X-linked adrenoleukodystrophy (X-ALD) a severe genetic disorder affecting the nervous system and adrenal glands. Mutations in PMP70 and related proteins particularly ALDP disrupt VLCFA degradation leading to toxic accumulation. This highlights the clinical significance of PMP70 and its potential as a therapeutic target in metabolic and neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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14 product images
Flow Cytometry - Anti-PMP70 antibody (ab3421)
Flow cytometry analysis of PMP70 in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab3421 at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 and re-suspended in PBS for FACS analysis.
Flow Cytometry - Anti-PMP70 antibody (ab3421)
Flow cytometry analysis of PMP70 in NIH/3T3 (Mouse embryo fibroblast cell line) whole cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab3421 at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 and re-suspended in PBS for FACS analysis.
Western blot - Anti-PMP70 antibody (ab3421)
All lanes: Western blot - Anti-PMP70 antibody (ab3421) at 1/500 dilution
Lane 1: Rat kidney tissue lysate at 20 µg
Lane 2: Rat liver tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Lane 4: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 10 µg
Lane 5: U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 10 µg
Predicted band size: 75 kDa
Flow Cytometry - Anti-PMP70 antibody (ab3421)
Flow cytometry analysis of PMP70 in HepG2 (Human liver hepatocellular carcinoma cell line) cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab3421 at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 and re-suspended in PBS for FACS analysis.
Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody (ab3421)
Knockdown of PMP70 was achieved by transfecting A431 (Human epidermoid carcinoma cell line) cells with PMP70 specific siRNA. Immunofluorescence analysis was performed on A-431 cells (untransfected, panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with PMP70 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with PMP70 Rabbit Polyclonal Antibody (5 μg/ml), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI, and Rhodamine Phalloidin (1:300) was used for cytoskeletal F-actin (red) staining. Reduction of specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to PMP70 (green). The images were captured at 60X magnification.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody (ab3421)
ab3421 (2µg/ml) staining PMP70 in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is apical cytoplasmic staining and staining of the endoplasmic reticulum in the epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Anti-PMP70 antibody (ab3421)
All lanes: Western blot - Anti-PMP70 antibody (ab3421) at 1/1000 dilution
Lane 1: Mouse lung tissue lysate at 25 µg
Lane 2: Mouse liver tissue lysate at 25 µg
Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 25 µg
Predicted band size: 75 kDa
Observed band size: 70 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody (ab3421)
Immunofluorescent analysis of PMP70 (green) in NIH/3T3 (Mouse embryo fibroblast cell line) ells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS for 15 minutes at room temperature. Cells were stained with ab3421 at 10 μg/mL in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody (ab3421)
Immunofluorescence analysis of PMP70 was performed using 70% confluent log phase A431 (Human epidermoid carcinoma cell line) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ab3421 at 5 μg/ml in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic (peroxisomal pattern) localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody (ab3421)
ICC/IF image of ab3421 stained human HeLa (Human epithelial adenocarcinoma cell line) cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3421, 1 μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody (ab3421)
Immunofluorescence analysis of PMP70 using ab3421 shows staining in HMVEC (Human microvascular endothelial cell line) cells.
Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody (ab3421)
Immunofluorescence analysis of PMP70 using ab3421 shows staining in NS-1 (Mouse myeloma cell line) cells.
Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody (ab3421)
Immunofluorescence analysis of PMP70 using ab3421 shows staining in P19 (Mouse embryonal carcinoma cell line) cells.
Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody (ab3421)
Immunofluorescence analysis of PMP70 using ab3421 shows staining in A549 (Human lung carcinoma cell line) cells.
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