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AB211533

Anti-PMP70 antibody [CL2524]

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(11 Publications)

Mouse Monoclonal PMP70 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCD3 aa 400-500.

View Alternative Names

PMP70, PXMP1, ABCD3, ATP-binding cassette sub-family D member 3, 70 kDa peroxisomal membrane protein

11 Images
Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533)

Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized U2OS cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533)

Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized HeLa cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533)

Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized A431 cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533)

Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized MCF7 cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human rectum tissue labeling PMP70 with ab211533 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fallopian tube tissue labeling PMP70 with ab211533 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling PMP70 with ab211533 at 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533)

Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized U251 cells labeling PMP70 with ab211533 at 4 μg/ml. Microtubule- and nuclear probes are visualized in red and blue respectively.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling PMP70 with ab211533 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling PMP70 with ab211533 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (AB211533)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling PMP70 with ab211533 at 1/200 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2524

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ABCD3 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

P28288

Epitope

ab211533 binds to an epitope located within the peptide sequence GVQVIPLIPGAGEII (aa 420-434) as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PMP70 also known as ABCD3 is a peroxisomal membrane protein with a molecular weight of approximately 70 kDa. It belongs to the ATP-binding cassette (ABC) transporter family and localizes on the peroxisomal membrane predominantly expressed in liver kidney and testes. PMP70 plays an important mechanical role in the transportation of very long-chain fatty acids (VLCFAs) across the peroxisomal membrane.
Biological function summary

This protein operates as part of a heterodimeric complex with its related transporter protein ALDP (ABCD1). PMP70 facilitates the import of VLCFAs into peroxisomes which are then subjected to β-oxidation. By doing this PMP70 contributes significantly to the metabolism of lipids maintaining internal cellular homeostasis.

Pathways

PMP70 integrates into the peroxisomal β-oxidation pathway alongside other proteins such as ALDP and ACSVL1. It ensures the proper flux of VLCFAs into peroxisomes which is essential for their breakdown and energy production. The protein also interacts with metabolic pathways linked to lipid biosynthesis and degradation illustrating its multifaceted role in cellular lipid management.

PMP70's dysfunction relates to X-linked adrenoleukodystrophy (X-ALD) a severe genetic disorder affecting the nervous system and adrenal glands. Mutations in PMP70 and related proteins particularly ALDP disrupt VLCFA degradation leading to toxic accumulation. This highlights the clinical significance of PMP70 and its potential as a therapeutic target in metabolic and neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that catalyzes the transport of long-chain fatty acids (LCFA)-CoA, dicarboxylic acids-CoA, long-branched-chain fatty acids-CoA and bile acids from the cytosol to the peroxisome lumen for beta-oxydation (PubMed : 11248239, PubMed : 24333844, PubMed : 25168382, PubMed : 29397936). Has fatty acyl-CoA thioesterase and ATPase activities (PubMed : 29397936). Probably hydrolyzes fatty acyl-CoAs into free fatty acids prior to their ATP-dependent transport into peroxisomes (By similarity). Thus, play a role in regulation of LCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation (PubMed : 24333844, PubMed : 25944712).
See full target information ABCD3
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Viruses 16: PubMed39205283

2024

The M2 Protein of the Influenza A Virus Interacts with PEX19 to Facilitate Virus Replication by Disrupting the Function of Peroxisome.

Applications

Unspecified application

Species

Unspecified reactive species

Tanbin Liu,Libin Liang,Pu Zhao,Weipeng Lin,Yichao Zhuang,Li Jiang,Hualan Chen,Chengjun Li

The Journal of cell biology 222: PubMed37737955

2023

Peroxisomal ROS control cytosolic Mycobacterium tuberculosis replication in human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Enrica Pellegrino,Beren Aylan,Claudio Bussi,Antony Fearns,Elliott M Bernard,Natalia Athanasiadi,Pierre Santucci,Laure Botella,Maximiliano G Gutierrez

Journal of neuroinflammation 20:43 PubMed36803838

2023

Amyloid-β accumulation in human astrocytes induces mitochondrial disruption and changed energy metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Marlena Zyśk,Chiara Beretta,Luana Naia,Abdulkhalek Dakhel,Linnea Påvénius,Hjalmar Brismar,Maria Lindskog,Maria Ankarcrona,Anna Erlandsson

iScience 25:105135 PubMed36185359

2022

Deficiency of peroxisomal NUDT7 stimulates lipogenesis in hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jinsoo Song,In-Jeoung Baek,Sujeong Park,Jinjoo Oh,Deokha Kim,Kyung Song,Mi Kyung Kim,Hye Won Lee,Byoung Kuk Jang,Eun-Jung Jin

The Journal of biological chemistry 298:102320 PubMed35933016

2022

The ARSACS disease protein sacsin controls lysosomal positioning and reformation by regulating microtubule dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Vincent Francis,Walaa Alshafie,Rahul Kumar,Martine Girard,Bernard Brais,Peter S McPherson

Autophagy 18:540-558 PubMed34074205

2021

Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Michael S Dahabieh,Fan Huang,Christophe Goncalves,Raúl Ernesto Flores González,Sathyen Prabhu,Alicia Bolt,Erminia Di Pietro,Elie Khoury,John Heath,Zi Yi Xu,Joelle Rémy-Sarrazin,Koren K Mann,Alexandre Orthwein,François-Michel Boisvert,Nancy Braverman,Wilson H Miller,Sonia V Del Rincón

Nature communications 11:6069 PubMed33247103

2020

An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Vallese,Cristina Catoni,Domenico Cieri,Lucia Barazzuol,Omar Ramirez,Valentina Calore,Massimo Bonora,Flavia Giamogante,Paolo Pinton,Marisa Brini,Tito Calì

Virulence 11:537-553 PubMed32434419

2020

Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generation.

Applications

Unspecified application

Species

Unspecified reactive species

Lei You,Junbo Chen,Weiyong Liu,Qi Xiang,Zhen Luo,Wenbiao Wang,Wei Xu,Kailang Wu,Qi Zhang,Yingle Liu,Jianguo Wu

eLife 8: PubMed31149896

2019

Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Elizabeth Torres,Ciara M Gallagher,Lars Plate,Meghna Gupta,Christina R Liem,Xiaoyan Guo,Ruilin Tian,Robert M Stroud,Martin Kampmann,Jonathan S Weissman,Peter Walter

Nature methods 15:685-688 PubMed30127504

2018

Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Strauss,Philipp C Nickels,Maximilian T Strauss,Vilma Jimenez Sabinina,Jan Ellenberg,Jeffrey D Carter,Shashi Gupta,Nebojsa Janjic,Ralf Jungmann
View all publications
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