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Mouse Monoclonal PMP70 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCD3 aa 400-500.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (AB211533), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human ABCD3 aa 400-500. The exact immunogen used to generate this antibody is proprietary information. Database link P28288

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/500.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
2.00000-10.00000 µg/mL
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that catalyzes the transport of long-chain fatty acids (LCFA)-CoA, dicarboxylic acids-CoA, long-branched-chain fatty acids-CoA and bile acids from the cytosol to the peroxisome lumen for beta-oxydation (PubMed:11248239, PubMed:24333844, PubMed:25168382, PubMed:29397936). Has fatty acyl-CoA thioesterase and ATPase activities (PubMed:29397936). Probably hydrolyzes fatty acyl-CoAs into free fatty acids prior to their ATP-dependent transport into peroxisomes (By similarity). Thus, play a role in regulation of LCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation (PubMed:24333844, PubMed:25944712).

Alternative names

PMP70, PXMP1, ABCD3, ATP-binding cassette sub-family D member 3, 70 kDa peroxisomal membrane protein

Recommended products

Mouse Monoclonal PMP70 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCD3 aa 400-500.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human ABCD3 aa 400-500. The exact immunogen used to generate this antibody is proprietary information. Database link P28288
Clone number
CL2524
Purification technique
Affinity purification Protein A
Epitope
ab211533 binds to an epitope located within the peptide sequence GVQVIPLIPGAGEII (aa 420-434) as determined by overlapping synthetic peptides.
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PMP70 also known as ABCD3 is a peroxisomal membrane protein with a molecular weight of approximately 70 kDa. It belongs to the ATP-binding cassette (ABC) transporter family and localizes on the peroxisomal membrane predominantly expressed in liver kidney and testes. PMP70 plays an important mechanical role in the transportation of very long-chain fatty acids (VLCFAs) across the peroxisomal membrane.

Biological function summary

This protein operates as part of a heterodimeric complex with its related transporter protein ALDP (ABCD1). PMP70 facilitates the import of VLCFAs into peroxisomes which are then subjected to β-oxidation. By doing this PMP70 contributes significantly to the metabolism of lipids maintaining internal cellular homeostasis.

Pathways

PMP70 integrates into the peroxisomal β-oxidation pathway alongside other proteins such as ALDP and ACSVL1. It ensures the proper flux of VLCFAs into peroxisomes which is essential for their breakdown and energy production. The protein also interacts with metabolic pathways linked to lipid biosynthesis and degradation illustrating its multifaceted role in cellular lipid management.

Associated diseases and disorders

PMP70's dysfunction relates to X-linked adrenoleukodystrophy (X-ALD) a severe genetic disorder affecting the nervous system and adrenal glands. Mutations in PMP70 and related proteins particularly ALDP disrupt VLCFA degradation leading to toxic accumulation. This highlights the clinical significance of PMP70 and its potential as a therapeutic target in metabolic and neurodegenerative diseases.

Product promise

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11 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized U251 cells labeling PMP70 with ab211533 at 4 μg/ml. Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized U2OS cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized MCF7 cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized A431 cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunofluorescent analysis of paraformalin-fixed, Triton X-100 permeabilized HeLa cells labeling PMP70 with ab211533 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling PMP70 with ab211533 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling PMP70 with ab211533 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fallopian tube tissue labeling PMP70 with ab211533 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling PMP70 with ab211533 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling PMP70 with ab211533 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody [CL2524] (ab211533)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human rectum tissue labeling PMP70 with ab211533 at 1/200 dilution.

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