Anti-PMS1 antibody [EPR27158-78] - BSA and Azide free

• WB

Lab

Western blot - Anti-PMS1 antibody [EPR27158-78] - BSA and Azide free (AB315799)

This data was developed using the same antibody clone in a different buffer formulation (ab315798).

Western blot : Anti-PMS1 antibody [EPR27158-78] (ab315798) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab315798 was shown to bind specifically to PMS1. A band was observed at 120 kDa in wild-type A549 cell lysates with no signal observed at this size in PMS1 knockout cell line. To generate this image, wild-type and PMS1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PMS1 antibody [EPR27158-78] (<a href='/en-us/products/primary-antibodies/pms1-antibody-epr27158-78-ab315798'>ab315798</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human PMS1 Knockout A549 cell line (<a href='/en-us/products/cell-lines/human-pms1-knockout-a549-cell-line-ab324214'>ab324214</a>) at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

A549 Cytoplasmic cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 120 kDa

false

• WB

Supplier Data

Western blot - Anti-PMS1 antibody [EPR27158-78] - BSA and Azide free (AB315799)

This data was developed using ab315798, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

The identity of the lower MW bands are unknown.

Exposure time : Lane 1 : 125 seconds; Lanes 2-4 : 48 seconds.

All lanes:

Western blot - Anti-PMS1 antibody [EPR27158-78] (<a href='/en-us/products/primary-antibodies/pms1-antibody-epr27158-78-ab315798'>ab315798</a>) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 2:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa

false

• WB

Supplier Data

Western blot - Anti-PMS1 antibody [EPR27158-78] - BSA and Azide free (AB315799)

This data was developed using ab315798, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

The identity of the lower MW band at approximately 40 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PMS1 antibody [EPR27158-78] (<a href='/en-us/products/primary-antibodies/pms1-antibody-epr27158-78-ab315798'>ab315798</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting PMS1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

true

Exposure time: 180s