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Rabbit Recombinant Monoclonal PMS2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

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Images

Western blot - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (AB214442), expandable thumbnail
  • Immunoprecipitation - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (AB214442), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (AB214442), expandable thumbnail
  • Immunoprecipitation - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (AB214442), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (AB214442), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

7 products for Alternative Version

Target data

Function

Component of the post-replicative DNA mismatch repair system (MMR) (PubMed:30653781, PubMed:35189042). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Possesses an ATPase activity, but in the absence of gross structural changes, ATP hydrolysis may not be necessary for proficient mismatch repair (PubMed:35189042).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal PMS2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR3947
Purification technique
Affinity purification Protein A
Dissociation constant
1.5 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab214442 is the carrier-free version of Anti-PMS2 antibody [EPR3947] ab110638.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PMS2 also known as Postmeiotic Segregation Increased 2 or PMS2 MutL homolog is a protein that participates in DNA mismatch repair. It weighs approximately 96 kDa and often identifies as a member of the MutL protein family. PMS2 is ubiquitously expressed in the body with higher abundances in tissues that undergo rapid proliferation or possess a high mitotic index.

Biological function summary

PMS2 operates as part of the DNA mismatch repair (MMR) complex. It collaborates with other MutL homologs including MLH1 to form a heterodimer which is essential for repairing DNA replication errors. It safeguards genomic integrity and prevents mutations from accumulating in dividing cells serving important functions in cellular viability and genetic stability.

Pathways

PMS2 is involved in the DNA damage response and cell cycle regulation. The protein plays a vital role in the mismatch repair (MMR) pathway. PMS2 partners primarily with MLH1 within this pathway and both proteins work in conjunction to recognize and initiate repair on erroneous DNA sequences that emerge during replication preventing illegitimate recombination and chromosomal rearrangements.

Associated diseases and disorders

PMS2 mutations occur frequently in Lynch syndrome an inherited cancer predisposition disorder and Turcot syndrome a condition associated with colorectal cancer and brain tumors. MLH1 frequently associates with PMS2 in these disorders as defects in either protein can impair mismatch repair leading to an increased risk of cancer.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

  • Western blot - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Western blot - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    PMS2 Western blot staining using rabbit Anti-PMS2 antibody

    This data was developed using the same antibody clone in a different buffer formulation (Anti-PMS2 antibody [EPR3947] ab110638).

    Lanes 1- 2: Merged signal (red and green). Green - Anti-PMS2 antibody [EPR3947] ab110638 observed at 120 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    Anti-PMS2 antibody [EPR3947] ab110638 was shown to react with PMS2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human PMS2 knockout HeLa cell line ab261776 (knockout cell lysate Human PMS2 knockout HeLa cell lysate ab257142) was used. Wild-type HeLa and PMS2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-PMS2 antibody [EPR3947] ab110638 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PMS2 antibody [EPR3947] (Anti-PMS2 antibody [EPR3947] ab110638) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: PMS2 knockout HeLa cell lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Lanes 1 - 2: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 96 kDa

    Observed band size: 120 kDa

  • Immunoprecipitation - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Immunoprecipitation - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    This data was developed using Anti-PMS2 antibody [EPR3947] ab110638, the same antibody clone in a different buffer formulation.
    Purified Anti-PMS2 antibody [EPR3947] ab110638 at 1/50 dilution (2μg) immunoprecipitating PMS2 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): Anti-PMS2 antibody [EPR3947] ab110638 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PMS2 antibody [EPR3947] ab110638 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/5000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 110 kDa
    Lower bands are degradation bands and fresh lysate is recommended.

    All lanes: Immunoprecipitation - Anti-PMS2 antibody [EPR3947] (Anti-PMS2 antibody [EPR3947] ab110638)

    Predicted band size: 96 kDa

  • Flow Cytometry (Intracellular) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    This data was developed using Anti-PMS2 antibody [EPR3947] ab110638, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling PMS2 with Purified ab214442 at 1:160 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Immunoprecipitation - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    This data was developed using Anti-PMS2 antibody [EPR3947] ab110638, the same antibody clone in a different buffer formulation.
    Purified Anti-PMS2 antibody [EPR3947] ab110638 at 1/50 dilution (2μg) immunoprecipitating PMS2 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): Anti-PMS2 antibody [EPR3947] ab110638 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PMS2 antibody [EPR3947] ab110638 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/5000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 110 kDa
    Lysate were made freshly and used in IP test immediately to minimize protein degradation. Incubation time was 2h.

    All lanes: Immunoprecipitation - Anti-PMS2 antibody [EPR3947] (Anti-PMS2 antibody [EPR3947] ab110638)

    Predicted band size: 96 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    This data was developed using Anti-PMS2 antibody [EPR3947] ab110638, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling PMS2 with Purified ab214442 at 1:200 dilution (8 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Western blot - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    This WB data was generated using the same anti-PMS2 antibody clone, EPR3947, in a different buffer formulation (cat# Anti-PMS2 antibody [EPR3947] ab110638).

    Lanes 1-2: Merged signal (red and green). Green - Anti-PMS2 antibody [EPR3947] ab110638 observed at 120 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-PMS2 antibody [EPR3947] ab110638 was shown to specifically react with PMS2 in wild-type HAP1 cells. No band was observed when PMS2 knockout samples were used. Wild-type and PMS2 knockout samples were subjected to SDS-PAGE. Anti-PMS2 antibody [EPR3947] ab110638 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PMS2 antibody [EPR3947] (Anti-PMS2 antibody [EPR3947] ab110638) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 30 µg

    Lane 2: PMS2 knockout HAP1 whole cell lysate at 30 µg

    Predicted band size: 96 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    This data was developed using Anti-PMS2 antibody [EPR3947] ab110638, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling PMS2 with Purified ab214442 at 1:600 (2.71 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    This data was developed using ab214442, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling PMS2 with Purified ab214442 at 1:600 (2.71 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    This data was developed using Anti-PMS2 antibody [EPR3947] ab110638, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling PMS2 with Purified ab214442 at 1:600 (2.71 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    Tissue Microarrays stained for "Anti-PMS2 antibody [EPR3947]" using "Anti-PMS2 antibody [EPR3947] ab110638"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with Anti-PMS2 antibody [EPR3947] ab110638 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    This data was developed using Anti-PMS2 antibody [EPR3947] ab110638, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of formalin fixed paraffin embedded human colon labelling PMS2 with Anti-PMS2 antibody [EPR3947] ab110638 at a concentration of 3µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    Anti-PMS2 antibody [EPR3947] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

  • Western blot - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442), expandable thumbnail

    Western blot - Anti-PMS2 antibody [EPR3947] - BSA and Azide free (ab214442)

    This data was developed using Anti-PMS2 antibody [EPR3947] ab110638, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 GADPH was used as a loading control.

    Lower bands are degradation bands and fresh lysate is recommended to get desired WB result.

    All lanes: Western blot - Anti-PMS2 antibody [EPR3947] (Anti-PMS2 antibody [EPR3947] ab110638) at 1/10000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, freshly made at 20 µg

    Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, freshly made at 20 µg

    Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 96 kDa

    Observed band size: 120 kDa

    Exposure time: 20s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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