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AB204800

Anti-PN-I antibody

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(2 Publications)

Rabbit Polyclonal PN-I antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NT5C3A aa 150-350.

View Alternative Names

NT5C3, P5N1, UMPH1, HSPC233, NT5C3A, Cytosolic 5'-nucleotidase 3A, 7-methylguanosine phosphate-specific 5'-nucleotidase, Cytosolic 5'-nucleotidase 3, Cytosolic 5'-nucleotidase III, Pyrimidine 5'-nucleotidase 1, Uridine 5'-monophosphate hydrolase 1, p36, 7-methylguanosine nucleotidase, cN-III, P5'N-1, P5N-1, PN-I

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PN-I antibody (AB204800)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PN-I antibody (AB204800)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human stomach tissue labeling PN-I with ab204800 at 1/50.

Western blot - Anti-PN-I antibody (AB204800)
  • WB

Supplier Data

Western blot - Anti-PN-I antibody (AB204800)

All lanes:

Western blot - Anti-PN-I antibody (ab204800) at 1/100 dilution

Lane 1:

Negative Control

Lane 2:

HEK lysate

Secondary

All lanes:

HRP-conjugated Swine Anti-Rabbit antibody at 1/3000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NT5C3A aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q9H0P0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PN-I commonly known as Protease Nexin I serves as a serine protease inhibitor. It has a molecular mass of approximately 43 kDa. PN-I expression occurs predominantly in the nervous system and various cell types such as fibroblasts and endothelial cells. It regulates the activity of multiple proteases like thrombin urokinase and plasminogen activator. This regulation helps to ensure a balance in protease activity.
Biological function summary

PN-I plays a significant role in maintaining extracellular matrix homeostasis and modulating cellular processes like migration and proliferation. It is part of a complex with proteases that it inhibits forming stable complexes to neutralize their activity. Through its actions PN-I influences cell signaling and tissue remodeling having effects that are essential for physiological processes.

Pathways

PN-I interacts with the fibrinolytic and coagulation pathways. It modulates these pathways by inhibiting enzymes such as thrombin and urokinase impacting processes like blood clot formation and degradation. In the coagulation cascade PN-I effects relate closely to proteins like thrombin affecting blood homeostasis and ensuring a proper response to vascular injury.

PN-I has links to conditions such as thrombosis and tissue fibrosis. Alterations in PN-I expression or function can contribute to pathological states by disrupting protease regulation leading to excessive clot formation or tissue scarring. PN-I interacts with proteins involved in these diseases such as plasminogen activators highlighting its importance in maintaining proteolytic balance in tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP) (PubMed : 24603684). CMP seems to be the preferred substrate (PubMed : 15968458).
See full target information NT5C3A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology research 9:665-681 PubMed33839687

2021

Chemotherapy Induces Tumor-Associated Macrophages that Aid Adaptive Immune Responses in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Owen Heath,Chiara Berlato,Eleni Maniati,Anissa Lakhani,Colin Pegrum,Panoraia Kotantaki,Samar Elorbany,Steffen Böhm,Simon T Barry,Alessandro Annibaldi,Desmond P Barton,Frances R Balkwill

Function (Oxford, England) 1:zqaa018 PubMed33074265

2020

Impaired Myocardial Energetics Causes Mechanical Dysfunction in Decompensated Failing Hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Lopez,Bahador Marzban,Xin Gao,Ellen Lauinger,Françoise Van den Bergh,Steven E Whitesall,Kimber Converso-Baran,Charles F Burant,Daniel E Michele,Daniel A Beard
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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