AB111710

Anti-PNAD antibody

Name: Anti-PNAD antibody (ab111710) | Abcam
Brand: Abcam Limited
SKU: AB111710
Price: 520 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal PNAD antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NTAN1 aa 1-250.

View Alternative Names

Protein N-terminal asparagine amidohydrolase, Protein NH2-terminal asparagine amidohydrolase, Protein NH2-terminal asparagine deamidase, PNAA, PNAD, Protein N-terminal Asn amidase, Protein N-terminal asparagine amidase, Protein NTN-amidase, NTAN1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNAD antibody (AB111710)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNAD antibody (AB111710)

ab111710 at 1/500 dilution staining PNAD in Formalin-fixed, paraffin-embedded Human Hepatoma tissue by Immunohistochemistry.

Unknown

Western blot - Anti-PNAD antibody (AB111710)

10% SDS-PAGE.

All lanes:

Western blot - Anti-PNAD antibody (ab111710) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 35 kDa,39 kDa,40 kDa,41 kDa,42 kDa,43 kDa,46 kDa,47 kDa,48 kDa,50 kDa,52 kDa,53 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NTAN1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q96AB6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>or Tris-EDTA buffer ( PH8.0)</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab111710 is purified by antigen-affinity chromatography.

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Peripheral Node Addressin (PNAd) is a glycoprotein recognized by its role in lymphocyte homing to lymph nodes. It is an important ligand for L-selectin on leukocytes facilitating their entry into lymphoid tissues. The PNAd marker is primarily located on the high endothelial venules (HEVs) where it guides lymphocyte trafficking. PNAd's molecular weight varies depending on its glycosylation patterns but is generally documented around 50 to 90 kDa.

Biological function summary

PNAd mediates the adhesion of lymphocytes to HEVs a critical step in the immune response promoting the recirculation of lymphocytes throughout the body. PNAd does not function as an independent entity often part of a multiprotein complex involving various glycoproteins and ligands that collectively ensure efficient immune surveillance. Its interaction with L-selectin is an essential event in lymphocyte adhesion under shear flow conditions seen in blood circulation.

Pathways

PNAd plays a role in the lymphocyte signaling pathways particularly associated with leukocyte extravasation. This pathway is integral for lymphocyte migration involving key signaling molecules and interactions with proteins like ICAM-1 and VCAM-1. PNAd's function in this pathway reflects its importance for maintaining effective immune system balance and proper lymphocyte distribution.

PNAd has involvement in inflammatory conditions such as rheumatoid arthritis and various lymphomas where aberrant lymphocyte trafficking occurs. In rheumatoid arthritis unusual PNAd expression on inflamed venules can lead to inappropriate lymphocyte accumulation in joints. Similarly in some lymphomas altered PNAd expression may associate with changes in lymphocyte migration patterns. Its interaction with proteins such as L-selectin occurs more frequently in these pathologies often exacerbating disease profiles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

N-terminal asparagine deamidase that mediates deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by NTAN1/PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn.

See full target information NTAN1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:701 PubMed41053006

2025

Lactate-mediated histone lactylation promotes melanoma angiogenesis via IL-33/ST2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mao Zhao,Yuxuan Qian,Lin He,Taoxin Peng,Hanbin Wang,Xiangxu Wang,Linhan Jiang,Jinrong Fan,Hengxiang Zhang,Di Qu,Qing Zhu,Hao Wang,Shida Zhang,Chenyang Li,Xiwen Dong,Xianya Zhao,Huina Wang,Yuqi Yang,Xiuli Yi,Tao Zhao,Yu Liu,Jianglin Zhang,Guoqiang Zhang,Qiong Shi,Tianwen Gao,Chunying Li,Weinan Guo

Journal of translational medicine 22:477 PubMed38764038

2024

TCL1A-expressing B cells are critical for tertiary lymphoid structure formation and the prognosis of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqiang Xie,Jinjin Lu,Yichen Chen,Xi Wang,Huanzi Lu,Qunxing Li,Nianqiang Jin,Jiankang He,Lingling Ou,Jia Ni,Yuqin Shen,Longquan Shao

Cell reports. Medicine 5:101448 PubMed38458196

2024

An immune cell map of human lung adenocarcinoma development reveals an anti-tumoral role of the Tfh-dependent tertiary lymphoid structure.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Wenhua You,Zhenwei Lan,Yijiu Ren,Shuangshu Gao,Shuchao Li,Wei-Wei Chen,Chunyu Huang,Yong Zeng,Nengming Xiao,Zeshuai Wang,Huikang Xie,Huan Ma,Yun Chen,Guangsuo Wang,Chang Chen,Hanjie Li

Frontiers in immunology 14:1334408 PubMed38259481

2024

Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Duo Wang,Liuying Huang,Danqi Qian,Yulin Cao,Xiaohan Wu,Peiwen Xu,Liang Ming,Junhui Tang,Zhaohui Huang,Yuan Yin,Leyuan Zhou

Human cell 36:1041-1053 PubMed36952210

2023

Bone marrow mesenchymal stem cells derived exosomal Lnc TUG1 promotes bone fracture recovery via miR-22-5p/Anxa8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Lihong Li,Rui Cui,Xiaoqing Chen,Haifeng Hu,Yuyu Qiu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PNAD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNAD antibody (AB111710)

Western blot - Anti-PNAD antibody (AB111710)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

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