Anti-Pneumolysin antibody [PLY-4]

• WB

Lab

Western blot - Anti-Pneumolysin antibody [PLY-4] (AB71810)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at a 1/200000 dilution. Anti-6X His tag® antibody [AD1.1.10](ab15149) used for total protein control.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Pneumolysin antibody [PLY-4] (ab71810) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 40 µg

Lane 2:

293T transfected with Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) expression vector containing a myc-His-tag® whole cell lysate at 40 µg

Lane 3:

293T transfected with Streptococcus pyogenes serotype M3 (strain SSI-1) expression vector containing a myc-His-tag® whole cell lysate at 40 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 53 kDa

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Exposure time: 180s

• WB

Unknown

Western blot - Anti-Pneumolysin antibody [PLY-4] (AB71810)

Coomassie Blue-stained gel (lane 1) and Western blots of purified recombinant PLY, 3 µg per lane, with mouse monoclonal antibodies PLY-4 (lane 2), PLY-5 (lane 3) and PLY-7 (lane 4); a PLY-irrelevant mouse monoclonal antibody 1.4G8.66 served as control (lane 5).

This image was generated from a different batch produced using hybridoma method

All lanes:

Western blot - Anti-Pneumolysin antibody [PLY-4] (ab71810)

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