Mouse Monoclonal Pneumolysin antibody. Suitable for WB and reacts with Recombinant full length protein - Streptococcus pneumoniae samples. Cited in 15 publications.
Images
![Western blot - Anti-Pneumolysin antibody [PLY-4] (AB71810), expandable thumbnail](https://content.abcam.com/products/images/pneumolysin-antibody-ply-4-ab71810--western-blot-img135852.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Pneumolysin antibody [PLY-4] (AB71810)")
Publications
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- PLoS pathogens 18:e10110202022A multiomics analysis of direct interkingdom dynamics between influenza A virus and Streptococcus pneumoniae uncovers host-independent changes to bacterial virulence fitness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaryann P Platt,Yi-Han Lin,Trevor Penix,Rosana Wiscovitch-Russo,Isha Vashee,Chris A Mares,Jason W Rosch,Yanbao Yu,Norberto Gonzalez-JuarbePubMed 36542660
- PLoS pathogens 16:e10090162020Structural insights into loss of function of a pore forming toxin and its role in pneumococcal adaptation to an intracellular lifestyle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDilip C Badgujar et. al.PubMed 33216805
- Scientific reports 10:173132020Hypervirulent pneumococcal serotype 1 harbours two pneumolysin variants with differential haemolytic activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStavros Panagiotou et. al.PubMed 33057054
- Journal of applied microbiology 130:843-8512020Betulin attenuates pneumolysin-induced cell injury and DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZ Qi,Y Guo,H Zhang,Q Yu,P ZhangPubMed 32621771
- Scientific reports 8:158462018Missing elimination via membrane vesicle shedding contributes to the diminished calcium sensitivity of listeriolysin O.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJana Maurer et. al.PubMed 30367146
- mBio 9:2018Immunomodulatory Effects of Pneumococcal Extracellular Vesicles on Cellular and Humoral Host Defenses.Applications:WB, ICC/IFReactive species:Unspecified reactive speciesMario Codemo et. al.PubMed 29636428
- PLoS pathogens 13:e10065822017Streptococcus pneumoniae in the heart subvert the host response through biofilm-mediated resident macrophage killing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnukul T Shenoy et. al.PubMed 28841717
- Chemical & pharmaceutical bulletin 65:538-5442017Morin Moderates the Biotoxicity of Pneumococcal Pneumolysin by Weakening the Oligomers' Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoran Zhao,Yonglin Zhou,Guizhen Wang,Dongxue Shi,Yonghong Zha,Pengfei Yi,Jianfeng WangPubMed 28566646
- American journal of respiratory cell and molecular biology 53:689-7022015Statin-conferred enhanced cellular resistance against bacterial pore-forming toxins in airway epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Statt,Jhen-Wei Ruan,Li-Yin Hung,Ching-Yun Chang,Chih-Ting Huang,Jae Hyang Lim,Jian-Dong Li,Reen Wu,Cheng-Yuan KaoPubMed 25874372
- PloS one 10:e01227532015Streptococcus pneumoniae induces autophagy through the inhibition of the PI3K-I/Akt/mTOR pathway and ROS hypergeneration in A549 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPu Li et. al.PubMed 25803050
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Constituents: 0.06% Tris buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | |
|---|---|
| Recombinant full length protein - Streptococcus pneumoniae | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Streptococcus pneumoniae | Dilution info 3 µg/mL | Notes - |
Target data
Recommended products
Mouse Monoclonal Pneumolysin antibody. Suitable for WB and reacts with Recombinant full length protein - Streptococcus pneumoniae samples. Cited in 15 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Constituents: 0.06% Tris buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- PLY-4
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pneumolysin also known as Ply or ply-004 is a toxin protein produced by Streptococcus pneumoniae a bacterium responsible for many infections. It has a molecular mass of approximately 53 kDa. Pneumolysin is a cholesterol-dependent cytolysin meaning that it targets and binds to cholesterol in host cell membranes leading to pore formation and cell lysis. The toxin is predominantly expressed in S. pneumoniae where it plays an essential role in pathogenicity.
- Biological function summary
Pneumolysin affects host cells by disrupting cellular integrity and immune responses. It is not part of a larger protein complex. Pneumolysin can inhibit cilia movement in the respiratory tract impairing the host’s ability to clear mucus and pathogens. This toxin also activates the host immune system by promoting the release of pro-inflammatory cytokines which can result in tissue damage.
- Pathways
Pneumolysin plays a significant role in the inflammatory response and cell apoptosis pathways. Within the inflammatory pathway it can trigger the host's immune cells to release cytokines such as interleukin-6 and tumor necrosis factor-alpha. This response leads to increased vascular permeability and leukocyte migration. Pneumolysin-mediated cell lysis also contributes to apoptosis impacting cell survival. The toxin's action relates it to proteins involved in the immune signaling cascades.
- Associated diseases and disorders
Pneumolysin plays a vital role in diseases such as pneumonia and meningitis both caused by S. pneumoniae. The disruption of host cell membranes and induction of inflammation contribute directly to disease symptoms. Pneumolysin interacts with proteins involved in the host's immune defense during infection. These interactions and its cytotoxic properties make pneumolysin a target for research into vaccine and therapeutic strategies against pneumococcal diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Pneumolysin antibody [PLY-4] (ab71810), expandable thumbnail](https://content.abcam.com/products/images/pneumolysin-antibody-ply-4-ab71810--western-blot-img135852.jpg "Western blot - Anti-Pneumolysin antibody [PLY-4] (ab71810)")
Western blot - Anti-Pneumolysin antibody [PLY-4] (ab71810)
Coomassie Blue-stained gel (lane 1) and Western blots of purified recombinant PLY, 3 µg per lane, with mouse monoclonal antibodies PLY-4 (lane 2), PLY-5 (lane 3) and PLY-7 (lane 4); a PLY-irrelevant mouse monoclonal antibody 1.4G8.66 served as control (lane 5).
All lanes: Western blot - Anti-Pneumolysin antibody [PLY-4] (ab71810)
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