Rabbit Recombinant Monoclonal PNK/PNKP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (AB170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--western-blot-img111481.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (AB170954)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (AB170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138972.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (AB170954)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (AB170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17763.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (AB170954)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (AB170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81793.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (AB170954)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (AB170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92640.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (AB170954)")
Publications
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- Nucleic acids research 48:6672-66842020Pathological mutations in PNKP trigger defects in DNA single-strand break repair but not DNA double-strand break repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlona Kalasova,Richard Hailstone,Janin Bublitz,Jovel Bogantes,Winfried Hofmann,Alejandro Leal,Hana Hanzlikova,Keith W CaldecottPubMed 32504494
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways (PubMed:10446192, PubMed:10446193, PubMed:15385968, PubMed:20852255, PubMed:28453785). Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone (PubMed:10446192, PubMed:10446193).
Alternative names
Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, PNKP
Recommended products
Rabbit Recombinant Monoclonal PNK/PNKP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR11934(B)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Polyribonucleotide 5'-hydroxyl-kinase 3'-phosphatase often known as PNK or PNKP protein is a dual-function enzyme necessary in DNA repair processes. PNKP with a mass of about 57 kDa performs both kinase and phosphatase activities. It removes 3'-phosphates and phosphorylates 5'-hydroxyl groups in polynucleotides preparing them for subsequent repair. This enzyme is expressed in various tissues with significant levels in the brain indicating its widespread importance in cellular processes.
- Biological function summary
PNKP protein is integral to maintaining genome stability by facilitating the repair of single-strand breaks and double-strand breaks in DNA. It is not part of a complex but often collaborates with other repair proteins through its enzymatic functions. PNKP ensures repaired DNA ends are properly processed to allow accurate ligation by DNA ligases contributing to the integrity of the genetic material in cells.
- Pathways
PNKP operates mainly in the DNA repair pathways specifically in Base Excision Repair (BER) and Non-Homologous End Joining (NHEJ). Within the BER pathway PNKP collaborates with proteins like DNA polymerase β and XRCC1 in processing damaged DNA. In the NHEJ pathway it associates with proteins like Ku and DNA-PKcs aligning for intricate repair of DNA double-strand breaks.
- Associated diseases and disorders
Mutations and malfunctions in PNKP are linked to neurological disorders and cancer. Dysfunctions in PNKP activity associate with neurodegenerative conditions like Microcephaly with Seizures due to its role in efficient DNA repair in neuronal tissues. Additionally its connection to DNA Repair pathways links PNKP malfunction with increased cancer risk. PNKP involvement with proteins like ATM and ATR signals its importance as these are key players in the cellular response to DNA damage particularly in preventing tumorigenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--western-blot-img111481.jpg "Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)")
Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
All lanes: Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: PC3 cell lysate at 10 µg
Lane 4: Human testis lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138972.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
ab170954 showing +ve staining in Human testis tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17763.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
ab170954 showing +ve staining in Human normal pancreas tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81793.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
ab170954 showing +ve staining in Human thyroid gland carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92640.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.
The use of an HRP/AP polymerized secondary antibody is recommended.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118989.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.
The use of an HRP/AP polymerized secondary antibody is recommended.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunocytochemistry-immunofluorescence-img14925.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)")
Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
Immunofluorescence analysis of PC3 cells, labeling PNK/PNKP using ab170954 at a 1/100 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118989.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)")
![Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954), expandable thumbnail](https://content.abcam.com/products/images/pnk-pnkp-antibody-epr11934b-ab170954--immunocytochemistry-immunofluorescence-img14925.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)")
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