AB250354

Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PNK/PNKP antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, PNKP

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

This data was developed using ab181107, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNK/PNKP with ab181107 at 1/100 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

This data was developed using ab181107, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PNK/PNKP with ab181107 at 1/100 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

This data was developed using ab181107, the same antibody clone in a different buffer formulation.

Western blot analysis of PNK/PNKP in immunoprecipitation pellets from HeLa cell lysate. ab181107 used at 1/20 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Right lane shows negative control.

All lanes:

Immunoprecipitation - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (<a href='/en-us/products/primary-antibodies/pnk-pnkp-antibody-epr11935-c-terminal-ab181107'>ab181107</a>)

Predicted band size: 57 kDa

false

Supplier Data

Western blot - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

This data was developed using ab181107, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (<a href='/en-us/products/primary-antibodies/pnk-pnkp-antibody-epr11935-c-terminal-ab181107'>ab181107</a>) at 1/2000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 60 kDa

false

Supplier Data

Western blot - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

This data was developed using ab181107, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (<a href='/en-us/products/primary-antibodies/pnk-pnkp-antibody-epr11935-c-terminal-ab181107'>ab181107</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

PC-3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 60 kDa

false

Unconjugated

Anti-PNK/PNKP antibody [EPR11935] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11935

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250354 is the carrier-free version of ab181107.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Polyribonucleotide 5'-hydroxyl-kinase 3'-phosphatase often known as PNK or PNKP protein is a dual-function enzyme necessary in DNA repair processes. PNKP with a mass of about 57 kDa performs both kinase and phosphatase activities. It removes 3'-phosphates and phosphorylates 5'-hydroxyl groups in polynucleotides preparing them for subsequent repair. This enzyme is expressed in various tissues with significant levels in the brain indicating its widespread importance in cellular processes.

Biological function summary

PNKP protein is integral to maintaining genome stability by facilitating the repair of single-strand breaks and double-strand breaks in DNA. It is not part of a complex but often collaborates with other repair proteins through its enzymatic functions. PNKP ensures repaired DNA ends are properly processed to allow accurate ligation by DNA ligases contributing to the integrity of the genetic material in cells.

Pathways

PNKP operates mainly in the DNA repair pathways specifically in Base Excision Repair (BER) and Non-Homologous End Joining (NHEJ). Within the BER pathway PNKP collaborates with proteins like DNA polymerase β and XRCC1 in processing damaged DNA. In the NHEJ pathway it associates with proteins like Ku and DNA-PKcs aligning for intricate repair of DNA double-strand breaks.

Mutations and malfunctions in PNKP are linked to neurological disorders and cancer. Dysfunctions in PNKP activity associate with neurodegenerative conditions like Microcephaly with Seizures due to its role in efficient DNA repair in neuronal tissues. Additionally its connection to DNA Repair pathways links PNKP malfunction with increased cancer risk. PNKP involvement with proteins like ATM and ATR signals its importance as these are key players in the cellular response to DNA damage particularly in preventing tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways (PubMed : 10446192, PubMed : 10446193, PubMed : 15385968, PubMed : 20852255, PubMed : 28453785). Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone (PubMed : 10446192, PubMed : 10446193).

See full target information PNKP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

Immunoprecipitation - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

Western blot - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

Western blot - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (AB250354)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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