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AB151151

Anti-PNLDC1 antibody

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(1 Publication)

Rabbit Polyclonal PNLDC1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PNLDC1 aa 250-350.

View Alternative Names

Poly(A)-specific ribonuclease PNLDC1, PARN-like domain-containing protein 1, Poly(A)-specific ribonuclease domain-containing protein 1, HsPNLDC1, PNLDC1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNLDC1 antibody (AB151151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNLDC1 antibody (AB151151)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling PNLDC1 with ab151151 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNLDC1 antibody (AB151151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNLDC1 antibody (AB151151)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling PNLDC1 with ab151151 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNLDC1 antibody (AB151151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNLDC1 antibody (AB151151)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling PNLDC1 with ab151151 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNLDC1 antibody (AB151151)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNLDC1 antibody (AB151151)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PNLDC1 with ab151151 at 1/200.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PNLDC1 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q8NA58

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PNLDC1 also referred to as PARN-like domain-containing 1 is a protein known for its role as a poly(A)-specific ribonuclease. It functions by trimming the poly(A) tails at the 3' end of RNA molecules which affects RNA stability and translation. PNLDC1 has an approximate mass of 61 kDa. Researchers have detected its expression predominantly in the testes indicating a role in germ cell development. This localization suggests PNLDC1's specific involvement in reproductive biology which marks its distinct function from its homologs.
Biological function summary

The activity of maintaining RNA stability places PNLDC1 as part of an exosome complex similar to the one where its related protein PARN functions. PNLDC1 likely contributes to spermatogenesis through its action in RNA processing. By trimming RNA tails it influences the maturation and maintenance of sperm cells highlighting its significance in reproduction. The protein's activity in RNA metabolism emphasizes its regulatory function in controlling gene expression at the RNA level.

Pathways

The significance of PNLDC1 in regulating RNA stability integrates it into essential biological processes such as the RNA decay pathway. The protein works alongside enzymes like Dicer which handle post-transcriptional control of RNA molecules synergizing to maintain RNA integrity. Within the RNA stability pathway its function intersects with RNA interference pathways sharing roles in ensuring correct RNA turnover and function within cells.

Researchers have associated PNLDC1 with certain reproductive impairments particularly spermatogenic failure. This failure links to suboptimal RNA processing where PNLDC1 function is important. Decreased activity of PNLDC1 can impact proteins like DAZL which play roles in germ cell development. Additionally disturbances in PNLDC1 function can contribute to testicular dysgenesis pointing to its potential as a marker for reproductive diseases and emphasizing its importance in diagnosing fertility issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails (PubMed : 27515512). Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development (PubMed : 27515512). May act as a regulator of multipotency in embryonic stem cells (By similarity). Is a critical factor for proper spermatogenesis, involved in pre-piRNAs processing to generate mature piRNAs (PubMed : 34347949).
See full target information PNLDC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 11:681366 PubMed34136404

2021

N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis Differentially Regulating Ubiquitination of Targets.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Zhu,Feng Wang,Haojie Li,Xiao Zhang,Qi Wu,Ya Liu,Mingping Qian,Susu Guo,Yueyue Yang,Xiangfei Xue,Fenyong Sun,Yongxia Qiao,Qiuhui Pan
View all publications
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