AB154282

Anti-PNMT antibody

Name: Anti-PNMT antibody (ab154282) | Abcam
Brand: Abcam Limited
SKU: AB154282

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(4 Publications)

Rabbit Polyclonal PNMT antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PNMT aa 50 to C-terminus.

View Alternative Names

PENT, PNMT, Phenylethanolamine N-methyltransferase, PNMTase, Noradrenaline N-methyltransferase

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNMT antibody (AB154282)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNMT antibody (AB154282)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded U87 xenograft labeling PNMT with ab154282 at 1/500.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PNMT aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11086

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PNMT also known as Phenylethanolamine N-methyltransferase functions as an enzyme responsible for the methylation of norepinephrine to synthesize epinephrine. It belongs to the methyltransferase family and weighs approximately 30 kDa. PNMT is highly expressed in the adrenal medulla particularly in chromaffin cells but also found in smaller amounts in some parts of the brain such as the medulla oblongata and retina. This distribution supports its role in catecholamine biosynthesis and regulation.

Biological function summary

The conversion of norepinephrine to epinephrine increases the functionality of PNMT in stress response mechanisms. PNMT plays an important role in the synthesis of catecholamines which are essential for modulating the fight-or-flight response. It does not form part of a larger protein complex but interacts with substrates and cofactors directly to catalyze its reactions. PNMT's activity is carefully regulated by factors including substrate availability and enzyme expression levels in tissues where it operates.

Pathways

Norepinephrine to epinephrine synthesis conducted by PNMT marks the final step in the catecholamine biosynthetic pathway. This pathway is critical in managing cardiovascular and metabolic functions. The protein connects extensively with enzymes such as tyrosine hydroxylase and dopamine β-hydroxylase within this pathway which are involved in prior steps of catecholamine synthesis. PNMT's activity and regulation are therefore essential for maintaining homeostasis in stress and energy metabolism.

PNMT connects directly to conditions like hypertension and certain phaeochromocytomas which arise from catecholamine excess or imbalance. Elevated levels of PNMT can be found in tumors linked to the adrenal medulla affecting the production of epinephrine. PNMT's interaction with enzymes like dopamine β-hydroxylase is relevant in understanding these disorders since imbalances in enzyme activity can lead to disrupted catecholamine synthesis contributing to disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L-methionine as the methyl donor (PubMed : 20496117). Other substrates include phenylethanolamine and octopamine (PubMed : 16277617, PubMed : 16363801, PubMed : 8812853). Also methylates normetanephrine (By similarity).

See full target information PNMT

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Endocrinology 166: PubMed40576438

2025

Canine Adrenomedullary and Pheochromocytoma Organoids: A Novel In Vitro Model.

Applications

Unspecified application

Species

Unspecified reactive species

Marit F van den Berg,Elpetra P M Timmermans-Sprang,Fleur C Viets,Lucas van den Berg,Fatima Danawar,Monique E van Wolferen,Hans S Kooistra,Guy C M Grinwis,Wilhelmina H A de Jong,Martijn van Faassen,Sara Galac

Journal of veterinary internal medicine 38:2415-2424 PubMed39115145

2024

Metabolomic profiling of pheochromocytomas in dogs: Catecholamine phenotype and tricarboxylic acid cycle metabolites.

Applications

Unspecified application

Species

Unspecified reactive species

Marit F van den Berg,Nicole Bechmann,Hans S Kooistra,Monique E van Wolferen,Elpetra P M Timmermans-Sprang,Mirko Peitzsch,Sara Galac

eLife 12: PubMed38407266

2024

Molecular classification and tumor microenvironment characteristics in pheochromocytomas.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Qin,Yawei Xu,Shimiao Yu,Wencong Han,Shiheng Fan,Wenxiang Ai,Kenan Zhang,Yizhou Wang,Xuehong Zhou,Qi Shen,Kan Gong,Luyang Sun,Zheng Zhang

Radiation research 189:441-445 PubMed29373090

2018

The Beta 2 Adrenergic Receptor Antagonist Timolol Improves Healing of Combined Burn and Radiation Wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Huguette Albrecht,Hsin-Ya Yang,Maija Kiuru,Saipiroon Maksaereekul,Blythe Durbin-Johnson,Michael S Wong,Thomas R Stevenson,David M Rocke,R Rivkah Isseroff

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PNMT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNMT antibody (AB154282)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise