AB167427

Anti-PNMT antibody [EPR7547(2)]

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal PNMT antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications.

View Alternative Names

PENT, PNMT, Phenylethanolamine N-methyltransferase, PNMTase, Noradrenaline N-methyltransferase

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (AB167427)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (AB167427)

Immunofluorescence analysis of K562 cells, labeling PNMT using ab167427 at a 1/50 dilution.

Unknown

Western blot - Anti-PNMT antibody [EPR7547(2)] (AB167427)

All lanes:

Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution

Lane 1:

K562 lysate at 10 µg

Lane 2:

Human adrenal gland lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-PNMT antibody [EPR7547(2)] (AB167427)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution

All lanes:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa,85 kDa

Observed band size: 31 kDa

false

Exposure time: 3min

Carrier free

Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7547(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PNMT also known as Phenylethanolamine N-methyltransferase functions as an enzyme responsible for the methylation of norepinephrine to synthesize epinephrine. It belongs to the methyltransferase family and weighs approximately 30 kDa. PNMT is highly expressed in the adrenal medulla particularly in chromaffin cells but also found in smaller amounts in some parts of the brain such as the medulla oblongata and retina. This distribution supports its role in catecholamine biosynthesis and regulation.

Biological function summary

The conversion of norepinephrine to epinephrine increases the functionality of PNMT in stress response mechanisms. PNMT plays an important role in the synthesis of catecholamines which are essential for modulating the fight-or-flight response. It does not form part of a larger protein complex but interacts with substrates and cofactors directly to catalyze its reactions. PNMT's activity is carefully regulated by factors including substrate availability and enzyme expression levels in tissues where it operates.

Pathways

Norepinephrine to epinephrine synthesis conducted by PNMT marks the final step in the catecholamine biosynthetic pathway. This pathway is critical in managing cardiovascular and metabolic functions. The protein connects extensively with enzymes such as tyrosine hydroxylase and dopamine β-hydroxylase within this pathway which are involved in prior steps of catecholamine synthesis. PNMT's activity and regulation are therefore essential for maintaining homeostasis in stress and energy metabolism.

PNMT connects directly to conditions like hypertension and certain phaeochromocytomas which arise from catecholamine excess or imbalance. Elevated levels of PNMT can be found in tumors linked to the adrenal medulla affecting the production of epinephrine. PNMT's interaction with enzymes like dopamine β-hydroxylase is relevant in understanding these disorders since imbalances in enzyme activity can lead to disrupted catecholamine synthesis contributing to disease pathogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L-methionine as the methyl donor (PubMed : 20496117). Other substrates include phenylethanolamine and octopamine (PubMed : 16277617, PubMed : 16363801, PubMed : 8812853). Also methylates normetanephrine (By similarity).

See full target information PNMT

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 27: PubMed36601769

2023

Nerve growth factor causes epinephrine release dysfunction by regulating phenotype alterations and the function of adrenal medullary chromaffin cells in mice with allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Liu,Jian-Ping Liang,Xiao-Lin Huang,Zhong Liu,An-Bing Zhang,Nian-Gen Deng,Zi-Feng Wei,Jun Wang

Oncotarget 12:49-60 PubMed33456713

2021

The noradrenergic profile of plasma metanephrine in neuroblastoma patients is reproduced in xenograft mice models and arise from PNMT downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Karim Abid,Maja Beck Popovic,Katia Balmas Bourloud,Jacqueline Schoumans,Joana Grand-Guillaume,Eric Grouzmann,Annick Mühlethaler-Mottet

American journal of physiology. Regulatory, integr 317:R407-R417 PubMed31242021

2019

Regulation of catecholamine release from the adrenal medulla is altered in deer mice () native to high altitudes.

Applications

Unspecified application

Species

Unspecified reactive species

Angela L Scott,Nicole A Pranckevicius,Colin A Nurse,Graham R Scott

Scientific data 4:170134 PubMed28949324

2017

Three-dimensional image reconstruction of distribution of Pnmt cell-derived cells in murine heart.

Applications

IHC-Fr

Species

Mouse

Haibo Ni,Yange Wang,William Crawford,Shanzhuo Zhang,Longxian Cheng,Henggui Zhang,Ming Lei

The Journal of endocrinology 230:309-23 PubMed27402067

2016

Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sushil K Mahata,Hong Zheng,Sumana Mahata,Xuefei Liu,Kaushik P Patel

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PNMT antibody [EPR7547(2)]

Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (AB167427)

Western blot - Anti-PNMT antibody [EPR7547(2)] (AB167427)

Western blot - Anti-PNMT antibody [EPR7547(2)] (AB167427)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise