Mouse Polyclonal PNPLA3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PNPLA3.
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Publications
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- Cellular and molecular gastroenterology and hepatology 11:389-4062020Liver Pyruvate Kinase Promotes NAFLD/NASH in Both Mice and Humans in a Sex-Specific Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarthickeyan Chella Krishnan,Raquel R Floyd,Simon Sabir,Dulshan W Jayasekera,Paola V Leon-Mimila,Anthony E Jones,Angel A Cortez,Varun Shravah,Miklós Péterfy,Linsey Stiles,Samuel Canizales-Quinteros,Ajit S Divakaruni,Adriana Huertas-Vazquez,Aldons J LusisPubMed 32942044
- Nutrients 9:2017The Combination of Blueberry Juice and Probiotics Ameliorate Non-Alcoholic Steatohepatitis (NASH) by Affecting SREBP-1c/PNPLA-3 Pathway via PPAR-α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Ren et. al.PubMed 28264426
- American journal of physiology. Endocrinology and 302:E1063-92012PNPLA3 is regulated by glucose in human hepatocytes, and its I148M mutant slows down triglyceride hydrolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Perttilä et. al.PubMed 22338072
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human PNPLA3. Database link Q9NST1
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
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Target data
Function
Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA (PubMed:22560221). Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides (PubMed:15364929, PubMed:20034933, PubMed:22560221). In vitro may express hydrolytic activity against glycerolipids triacylglycerol, diacylglycerol and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety (PubMed:21878620). However, the triacylglycerol hydrolase activity is controversial and may be very low (PubMed:22560221). Possesses phospholipase A2 activity (PubMed:15364929).
Alternative names
ADPN, C22orf20, PNPLA3, 1-acylglycerol-3-phosphate O-acyltransferase PNPLA3, Acylglycerol transacylase, Adiponutrin, Calcium-independent phospholipase A2-epsilon, Lysophosphatidic acid acyltransferase, Patatin-like phospholipase domain-containing protein 3, iPLA2-epsilon
Recommended products
Mouse Polyclonal PNPLA3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PNPLA3.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human PNPLA3. Database link Q9NST1
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PNPLA3 also known as patatin-like phospholipase domain-containing protein 3 is an enzyme that catalyzes the hydrolysis of ester bonds in glycerophospholipids. This protein has a molecular mass of about 53 kDa and is mainly expressed in liver tissue as well as in adipose tissue. It plays a role in lipid remodeling and triglyceride metabolism. The gene for PNPLA3 is located on chromosome 22 and its expression levels can influence its function in cellular lipid dynamics.
- Biological function summary
PNPLA3 impacts lipid metabolism and storage in the liver. It does not function as part of a complex but rather exerts its effects independently. PNPLA3 modifies lipid droplet contents by converting triglyceride substrates. This activity helps regulate lipolysis affecting lipid droplet size and number in liver cells. Its function ensures a balance in hepatic lipid levels which is important for maintaining proper liver function and overall metabolic health.
- Pathways
PNPLA3 is integral to lipid metabolic pathways and energy homeostasis. It interacts with key players in triglyceride metabolism such as DGAT2 and PNPLA2. These proteins especially DGAT2 help in the transformation and storage of triglycerides reflecting PNPLA3's role in regulating lipid balance. PNPLA3's interactions and functions in these pathways highlight its part in energy storage and utilization processes.
- Associated diseases and disorders
PNPLA3 genetic variants associate with non-alcoholic fatty liver disease (NAFLD) and liver fibrosis. Its role in these conditions relates to its impact on liver triglyceride content. The I148M variant of PNPLA3 has a notable connection to an increased risk of developing NAFLD. Additionally PNPLA3 interacts with proteins involved in fibrogenesis pathways such as TGF-β which influences the progression of liver disease towards fibrosis. Understanding these interactions helps researchers develop targeted therapies for liver conditions.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PNPLA3 antibody (ab69170)
All lanes: Western blot - Anti-PNPLA3 antibody (ab69170) at 1/500 dilution
Lane 1: PNPLA3 transfected 293T cell lysate at 25 µg
Lane 2: Non-transfected 293T cell lysate at 25 µg
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
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