AB81874

Anti-PNPLA3 antibody

Name: Anti-PNPLA3 antibody (ab81874) | Abcam
Brand: Abcam Limited
SKU: AB81874
Price: 360 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(18 Publications)

Rabbit Polyclonal PNPLA3 antibody. Suitable for WB and reacts with Human samples. Cited in 18 publications.

View Alternative Names

ADPN, C22orf20, PNPLA3, 1-acylglycerol-3-phosphate O-acyltransferase PNPLA3, Acylglycerol transacylase, Adiponutrin, Calcium-independent phospholipase A2-epsilon, Lysophosphatidic acid acyltransferase, Patatin-like phospholipase domain-containing protein 3, iPLA2-epsilon

2 Images

Unknown

Western blot - Anti-PNPLA3 antibody (AB81874)

Blocking buffer : 2% BSA

Gel type : MOPS

Exposure Time : 12 minutes

All lanes:

Western blot - Anti-PNPLA3 antibody (ab81874) at 1 µg/mL

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

HepG2 whole cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 53 kDa

Observed band size: 35 kDa,55 kDa,70 kDa

false

Lab

Western blot - Anti-PNPLA3 antibody (AB81874)

The PNPLA3 sequence contains two glycosylation sites (Swissprot data). The additional band close to the band of interest might be a glycosylated form of PNPLA3. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

ab81874 at 1 µg/mL

Lane 1:

Human liver at 10 µg

Lane 2:

Human whole cell lysate HepG2 at 10 µg

Secondary

All lanes:

<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a> at 1/5000 dilution

Predicted band size: 53 kDa

Observed band size: 120 kDa,48 kDa,52 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PNPLA3 also known as patatin-like phospholipase domain-containing protein 3 is an enzyme that catalyzes the hydrolysis of ester bonds in glycerophospholipids. This protein has a molecular mass of about 53 kDa and is mainly expressed in liver tissue as well as in adipose tissue. It plays a role in lipid remodeling and triglyceride metabolism. The gene for PNPLA3 is located on chromosome 22 and its expression levels can influence its function in cellular lipid dynamics.

Biological function summary

PNPLA3 impacts lipid metabolism and storage in the liver. It does not function as part of a complex but rather exerts its effects independently. PNPLA3 modifies lipid droplet contents by converting triglyceride substrates. This activity helps regulate lipolysis affecting lipid droplet size and number in liver cells. Its function ensures a balance in hepatic lipid levels which is important for maintaining proper liver function and overall metabolic health.

Pathways

PNPLA3 is integral to lipid metabolic pathways and energy homeostasis. It interacts with key players in triglyceride metabolism such as DGAT2 and PNPLA2. These proteins especially DGAT2 help in the transformation and storage of triglycerides reflecting PNPLA3's role in regulating lipid balance. PNPLA3's interactions and functions in these pathways highlight its part in energy storage and utilization processes.

PNPLA3 genetic variants associate with non-alcoholic fatty liver disease (NAFLD) and liver fibrosis. Its role in these conditions relates to its impact on liver triglyceride content. The I148M variant of PNPLA3 has a notable connection to an increased risk of developing NAFLD. Additionally PNPLA3 interacts with proteins involved in fibrogenesis pathways such as TGF-β which influences the progression of liver disease towards fibrosis. Understanding these interactions helps researchers develop targeted therapies for liver conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs : arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA (PubMed : 22560221). Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides (PubMed : 15364929, PubMed : 20034933, PubMed : 22560221). In vitro may express hydrolytic activity against glycerolipids triacylglycerol, diacylglycerol and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety (PubMed : 21878620). However, the triacylglycerol hydrolase activity is controversial and may be very low (PubMed : 22560221). Possesses phospholipase A2 activity (PubMed : 15364929).

See full target information PNPLA3

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Journal of internal medicine 297:47-59 PubMed39560367

2024

Statin-associated regulation of hepatic PNPLA3 in patients without known liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Osman Ahmed,Vladimir S Shavva,Laura Tarnawski,Wanmin Dai,Filip Borg,Viggo V Olofsson,Ting Liu,Peter Saliba-Gustafsson,Christian Simini,Matteo Pedrelli,Otto Bergman,Giuseppe Danilo Norata,Paolo Parini,Anders Franco-Cereceda,Per Eriksson,Stephen G Malin,Hanna M Björck,Peder S Olofsson

International journal of endocrinology 2023:2872408 PubMed36825197

2023

PNPLA3 148M/M Is More Susceptible to Palmitic Acid-Induced Endoplasmic Reticulum Stress-Associated Apoptosis in HepG2 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzhi Chen,Xuemei Yan,Tian Wang,Hongrong Deng,Xiaojie Deng,Fen Xu,Hua Liang

Nature communications 13:6577 PubMed36323699

2022

Phosphorylation of 17β-hydroxysteroid dehydrogenase 13 at serine 33 attenuates nonalcoholic fatty liver disease in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Su,Sijin Wu,Yongliang Yang,Yanlin Guo,Haibo Zhang,Jie Su,Lei Chen,Zhuo Mao,Rongfeng Lan,Rong Cao,Chunjiong Wang,Hu Xu,Cong Zhang,Sha Li,Min Gao,Xiaocong Chen,Zhiyou Zheng,Bing Wang,Yi'ao Liu,Zuojun Liu,Zimei Wang,Baohua Liu,Xinmin Fan,Xiaoyan Zhang,Youfei Guan

Scientific reports 12:888 PubMed35042927

2022

Responsiveness of PNPLA3 and lipid-related transcription factors is dependent upon fatty acid profile in primary bovine hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia J Erb,Tawny L Chandler,Heather M White

Scientific reports 11:2839 PubMed33531537

2021

Greater liver PNPLA3 protein abundance in vivo and in vitro supports lower triglyceride accumulation in dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan S Pralle,Sophia J Erb,Henry T Holdorf,Heather M White

Endocrine :517-525 PubMed32862405

2020

PNPLA3 I148M is involved in the variability in anti-NAFLD response to exenatide.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzhi Chen,Xuemei Yan,Xiao Xu,Shuhua Yuan,Fen Xu,Hua Liang

Biochimica et biophysica acta. Molecular and cell 1865:158730 PubMed32361002

2020

Lysosomal acid lipase is the major acid retinyl ester hydrolase in cultured human hepatic stellate cells but not essential for retinyl ester degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Carina Wagner,Victoria Hois,Laura Pajed,Lisa-Maria Pusch,Heimo Wolinski,Michael Trauner,Robert Zimmermann,Ulrike Taschler,Achim Lass

Liver international : official journal of the International Association for the Study of the Liver 40:1130-1141 PubMed32125756

2020

PNPLA3 I148M gene variant and chronic kidney disease in type 2 diabetic patients with NAFLD: Clinical and experimental findings.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Mantovani,Alice Taliento,Chiara Zusi,Guido Baselli,Daniele Prati,Simona Granata,Gianluigi Zaza,Antonio Colecchia,Claudio Maffeis,Christopher D Byrne,Luca Valenti,Giovanni Targher

Liver international : official journal of the Inte 40:1098-1110 PubMed32043752

2020

Metabolic regulation of hepatic PNPLA3 expression and severity of liver fibrosis in patients with NASH.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca V Bruschi,Matteo Tardelli,Merima Herac,Thierry Claudel,Michael Trauner

Journal of cellular and molecular medicine 24:1541-1552 PubMed31793207

2019

PNPLA3 I148M mediates the regulatory effect of NF-kB on inflammation in PA-treated HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhua Yuan,Hongxia Liu,Ding Yuan,Jing Xu,Yunzhi Chen,Xiao Xu,Fen Xu,Hua Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PNPLA3 antibody

Western blot - Anti-PNPLA3 antibody (AB81874)

Western blot - Anti-PNPLA3 antibody (AB81874)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

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