Anti-PNPLA3 I148M variant antibody [EPR30478-510] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNPLA3 I148M variant antibody [EPR30478-510] - BSA and Azide free (AB325023)

This data was developed using ab325019, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PNPLA3 I148M variant with ab325019 at 1/2000 (0.265 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no stianing on human cerebrum. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNPLA3 I148M variant antibody [EPR30478-510] - BSA and Azide free (AB325023)

This data was developed using ab325019, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling PNPLA3 I148M variant with ab325019 at 1/2000 (0.265 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no stianing on human cardiac muscle. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNPLA3 I148M variant antibody [EPR30478-510] - BSA and Azide free (AB325023)

This data was developed using ab325019, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Human PNPLA3(mutant I148M) expression vector containing a Myc-His tag. (B) HEK-293T transfected with a Human S1PR1expression vector containing a Myc-His tag. (C) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling PNPLA3 I148M variant with ab325019 at 1/2000 (0.265 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEK-293T transfected with a Human PNPLA3(mutant I148M) expression vector containing a Myc-His tag (image A), no staining on HEK-293T transfected with a Human S1PR1expression vector containing a Myc-His tag (image B) and HEK-293T transfected with empty vector containing a Myc-His tag (image C). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-PNPLA3 I148M variant antibody [EPR30478-510] - BSA and Azide free (AB325023)

This data was developed using ab325019, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PNPLA3 I148M variant antibody [EPR30478-510] (<a href='/en-us/products/primary-antibodies/pnpla3-i148m-variant-antibody-epr30478-510-ab325019'>ab325019</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a human PNPLA3(I148M mutation) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with a human PNPLA3 (WT) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 52 kDa,36 kDa,53 kDa

false

Exposure time: 180s