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AB125879

Anti-PNPLA4 antibody

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(1 Publication)

Rabbit Polyclonal PNPLA4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PNPLA4 aa 1-250.

View Alternative Names

DXS1283E, GS2, PNPLA4, Patatin-like phospholipase domain-containing protein 4, Calcium-independent phospholipase A2-eta, Protein GS2, iPLA2-eta

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNPLA4 antibody (AB125879)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNPLA4 antibody (AB125879)

Immunohistochemical analysis of paraffin-embedded Hepatoma, using ab125879 antibody at 1 : 500 dilution

Western blot - Anti-PNPLA4 antibody (AB125879)
  • WB

Unknown

Western blot - Anti-PNPLA4 antibody (AB125879)

12% SDS PAGE

All lanes:

Western blot - Anti-PNPLA4 antibody (ab125879) at 1/500 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 26 kDa,27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PNPLA4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P41247

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PNPLA4 also known as patatin-like phospholipase domain-containing protein 4 or GS2 is a lipid-modifying enzyme with a molecular weight of approximately 53 kDa. It plays a role in lipid metabolism by catalyzing the hydrolysis of phospholipids to glycerolipids and free fatty acids. You can find PNPLA4 widely expressed in various tissues including the liver adipose tissue and skin. This enzyme contributes to maintaining lipid homeostasis and is part of a larger family of patatin-like phospholipases that show distinct functions in lipid metabolism.
Biological function summary

PNPLA4 participates in lipid remodeling within cellular membranes and storage lipid pools. The protein facilitates the mobilization and alteration of lipid molecules a process important for normal cellular function and response to environmental changes. PNPLA4 may operate as a part of larger enzymatic complexes involved in lipid droplet formation and utilization. By impacting lipid dynamics PNPLA4 influences cellular energy regulation and membrane composition which is critical for various physiological processes such as energy storage and cell signaling.

Pathways

The PNPLA4 protein is a component of the glycerolipid metabolism and fatty acid degradation pathways. It functions alongside proteins such as ATGL (adipose triglyceride lipase) and HSL (hormone-sensitive lipase) which also regulate lipid breakdown and mobilization. PNPLA4's enzymatic activity and interactions contribute to the regulation of energy balance fatty acid availability and lipid signaling affecting metabolic processes and overall cellular health.

PNPLA4 associates with disorders related to lipid metabolism such as metabolic syndrome and liver steatosis. Altered PNPLA4 function can disrupt lipid homeostasis leading to excessive lipid accumulation within tissues particularly the liver. This dysfunction often correlates with a cascade of metabolic disturbances. PNPLA4 also connects to APOE another lipid-related protein implicated in metabolic and cardiovascular diseases. Understanding PNPLA4’s role in these conditions could offer insights into potential therapeutic approaches to manage or alleviate related metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has abundant triacylglycerol lipase activity (PubMed : 15364929, PubMed : 16150821, PubMed : 17603008). Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides (PubMed : 17603008). Additionally possesses acylglycerol transacylase and phospholipase A2 activities (PubMed : 15364929, PubMed : 17603008).
See full target information PNPLA4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Archives of toxicology 98:807-820 PubMed38175295

2024

In vitro evaluation of the reductive carbonyl idarubicin metabolism to evaluate inhibitors of the formation of cardiotoxic idarubicinol via carbonyl and aldo-keto reductases.

Applications

Unspecified application

Species

Unspecified reactive species

Gzona Bajraktari-Sylejmani,Julia Sophie Oster,Jürgen Burhenne,Walter Emil Haefeli,Max Sauter,Johanna Weiss
View all publications
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Product promise

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