Rabbit Polyclonal PNPLA8 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PNPLA8 aa 400-700.
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Publications
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- Diabetologia 64:1642-16592021Exercise training improves mitochondrial respiration and is associated with an altered intramuscular phospholipid signature in women with obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy E Mendham,Julia H Goedecke,Yingxu Zeng,Steen Larsen,Cindy George,Jon Hauksson,Melony C Fortuin-de Smidt,Alexander V Chibalin,Tommy Olsson,Elin ChorellPubMed 33770195
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PNPLA8 aa 400-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NP80
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Calcium-independent and membrane-bound phospholipase, that catalyzes the esterolytic cleavage of fatty acids from glycerophospholipids to yield free fatty acids and lysophospholipids, hence regulating membrane physical properties and the release of lipid second messengers and growth factors (PubMed:10744668, PubMed:10833412, PubMed:15695510, PubMed:15908428, PubMed:17213206, PubMed:18171998, PubMed:28442572). Hydrolyzes phosphatidylethanolamine, phosphatidylcholine and probably phosphatidylinositol with a possible preference for the former (PubMed:15695510). Has also a broad substrate specificity in terms of fatty acid moieties, hydrolyzing saturated and mono-unsaturated fatty acids at nearly equal rates from either the sn-1 or sn-2 position in diacyl phosphatidylcholine (PubMed:10744668, PubMed:10833412, PubMed:15695510, PubMed:15908428). However, has a weak activity toward polyunsaturated fatty acids at the sn-2 position, and thereby favors the production of 2-arachidonoyl lysophosphatidylcholine, a key branch point metabolite in eicosanoid signaling (PubMed:15908428). On the other hand, can produce arachidonic acid from the sn-1 position of diacyl phospholipid and from the sn-2 position of arachidonate-containing plasmalogen substrates (PubMed:15908428). Therefore, plays an important role in the mobilization of arachidonic acid in response to cellular stimuli and the generation of lipid second messengers (PubMed:15695510, PubMed:15908428). Can also hydrolyze lysophosphatidylcholine (PubMed:15695510). In the mitochondrial compartment, catalyzes the hydrolysis and release of oxidized aliphatic chains from cardiolipin and integrates mitochondrial bioenergetics and signaling. It is essential for maintaining efficient bioenergetic mitochondrial function through tailoring mitochondrial membrane lipid metabolism and composition (PubMed:28442572).
Alternative names
IPLA22, IPLA2G, BM-043, PNPLA8, Calcium-independent phospholipase A2-gamma, Intracellular membrane-associated calcium-independent phospholipase A2 gamma, PNPLA-gamma, Patatin-like phospholipase domain-containing protein 8, iPLA2-2, iPLA2-gamma
Recommended products
Rabbit Polyclonal PNPLA8 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PNPLA8 aa 400-700.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PNPLA8 aa 400-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NP80
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PNPLA8 also known as Patatin-like phospholipase domain-containing protein 8 or iPLA2γ is a protein involved in lipid metabolism. It has a molecular mass of approximately 88 kDa. This protein is widely expressed with significant presence in cardiac and skeletal muscle tissues. PNPLA8 plays a role in hydrolyzing phospholipids catalyzing the release of fatty acids which is an important process in cellular lipid metabolism.
- Biological function summary
PNPLA8 participates in the remodeling of membrane phospholipids a process necessary for maintaining cellular membrane integrity and function. It is a part of a larger protein complex that interacts with other enzymes involved in phospholipid metabolism. By facilitating the breakdown of phospholipids PNPLA8 impacts the regulation of signaling lipids which are critical for cellular responses and energy production.
- Pathways
The PNPLA8 protein contributes significantly to the phospholipid signaling and lipid degradation pathways. It plays an essential role in the mitochondrial pathway impacting mitochondrial fatty acid oxidation. PNPLA8 interacts with other proteins involved in these pathways such as PPARα a regulator of fatty acid metabolism highlighting its importance in energy homeostasis and cell signaling processes.
- Associated diseases and disorders
PNPLA8 links to metabolic and neuromuscular disorders. Mutations or dysfunctions in PNPLA8 have associations with cardiomyopathy and Barth syndrome due to impaired lipid homeostasis and mitochondrial integrity. PNPLA8 further interacts with tafazzin (TAZ) an enzyme also involved in phospholipid metabolism which connects to the pathogenesis of these disorders. By studying PNPLA8 researchers aim to better understand the molecular basis of lipid-related diseases and explore potential therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PNPLA8 antibody (ab154233)
7.5% SDS PAGE
All lanes: Western blot - Anti-PNPLA8 antibody (ab154233) at 1/1000 dilution
All lanes: Jurkat whole cell lysate at 30 µg
Predicted band size: 88 kDa
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