AB157109

Anti-PNPT1 antibody

Name: Anti-PNPT1 antibody (ab157109) | Abcam
Brand: Abcam Limited
SKU: AB157109
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal PNPT1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PNPT1 aa 700-750.

View Alternative Names

PNPASE, PNPT1, 3'-5' RNA exonuclease OLD35, PNPase old-35, Polynucleotide phosphorylase 1, Polynucleotide phosphorylase-like protein, PNPase 1

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Immunoprecipitation - Anti-PNPT1 antibody (AB157109)

Detection of PNPT1 by Western blot of immunoprecipitate. Immunoprecipitation of 293T whole cell lysate at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following :
Lane 1 : IP with an alternative Rabbit Anti-PNPT1 antibody.
Lane 2 : IP with ab157109.
Lane 3 : Control IgG.
The subsequent Western blot of immunoprecipitates was done using ab157109 at 1 μg/ml.
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PNPT1 antibody (ab157109)

All lanes:

293T whole cell lysate

Predicted band size: 86 kDa

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Unknown

Western blot - Anti-PNPT1 antibody (AB157109)

All lanes:

Western blot - Anti-PNPT1 antibody (ab157109) at 0.4 µg/mL

Lane 1:

293T cell lysate at 50 µg

Lane 2:

293T cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 50 µg

Lane 4:

Jurkat cell lysate at 50 µg

Predicted band size: 86 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PNPT1 aa 700-750. The exact immunogen used to generate this antibody is proprietary information.

Q8TCS8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PNPT1 also known as polyribonucleotide nucleotidyltransferase 1 or mitochondrial RNA polymerase is an enzyme with a molecular weight of approximately 85 kDa. The protein is widely expressed in cellular mitochondria and cytoplasm where it performs its essential functions in RNA processing. It aids in the degradation and processing of mitochondrial RNA reflecting its significant role in mitochondrial function. The protein's activity relies heavily on its conserved domains that are necessary for effective RNA binding and hydrolysis.

Biological function summary

PNPT1 plays a critical role in maintaining mitochondrial gene expression and is a component of the RNA-degrading exosome complex. This complex is involved in the surveillance and breakdown of defective RNA molecules thereby ensuring the integrity and balance of the mitochondrial transcriptome. By participating in these processes PNPT1 helps to enhance the overall mitochondrial function which is vital for energy production and cell metabolism.

Pathways

PNPT1 significantly contributes to the mitochondrial RNA decay pathway and the broader RNA processing pathways related to mitochondrial maintenance. It interacts with other proteins such as polynucleotide phosphorylase domain-containing proteins which are involved in similar RNA processing functions. This integration highlights the coordinated network of proteins that cooperate to ensure proper mitochondrial RNA metabolism affecting both the quality and quantity of the RNA molecules available for translation.

Mutations or dysregulations in PNPT1 are linked to mitochondrial disorders such as autosomal recessive combined oxidative phosphorylation deficiency. These disorders often exhibit symptoms like muscle weakness developmental delays and other systemic issues due to compromised energy production. Additionally studies indicate a possible connection between PNPT1 dysfunction and neurodegenerative disorders where its interaction with proteins like TYMP (thymidine phosphorylase) can lead to mitochondrial dysfunction and subsequent disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein implicated in numerous RNA metabolic processes (PubMed : 29967381, PubMed : 39019044). Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction (PubMed : 29967381, PubMed : 39019044). Mitochondrial intermembrane factor with RNA-processing exoribonulease activity (PubMed : 29967381, PubMed : 39019044). Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner (PubMed : 29967381, PubMed : 39019044). Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PubMed : 29967381, PubMed : 39019044). Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Also plays a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.

See full target information PNPT1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadu6271 PubMed40880474

2025

Glutamate utilization fuels rapid production of mitochondrial ROS in dendritic cells and drives systemic inflammation during tularemia.

Applications

Unspecified application

Species

Unspecified reactive species

Ivo Fabrik,Petra Spidlova,Lukas Prchal,Daniela Fabrikova,Ina Viduka,Valentina Marecic,Vlada Filimonenko,Radek Sleha,Marie Vajrychova,Rudolf Kupcik,Ondrej Soukup,Tomas Rousar,Anetta Härtlova,Marina Santic,Jiri Stulik

Molecular cell 84:2935-2948.e7 PubMed39019044

2024

RNA 5-methylcytosine marks mitochondrial double-stranded RNAs for degradation and cytosolic release.

Applications

Unspecified application

Species

Unspecified reactive species

Sujin Kim,Stephanie Tan,Jayoung Ku,Tria Asri Widowati,Doyeong Ku,Keonyong Lee,Kwontae You,Yoosik Kim

JCI insight 4: PubMed30721151

2019

Epigenetic modulation of β cells by interferon-α via PNPT1/mir-26a/TET2 triggers autoimmune diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Mihaela Stefan-Lifshitz,Esra Karakose,Lingguang Cui,Abora Ettela,Zhengzi Yi,Weijia Zhang,Yaron Tomer

Cancer research 78:1169-1183 PubMed29311158

2018

Long Noncoding RNA pancEts-1 Promotes Neuroblastoma Progression through hnRNPK-Mediated β-Catenin Stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Xiaojing Wang,Hong Mei,Erhu Fang,Lin Ye,Huajie Song,Feng Yang,Huanhuan Li,Kai Huang,Liduan Zheng,Qiangsong Tong

RNA (New York, N.Y.) 24:332-341 PubMed29263133

2017

miR-222 isoforms are differentially regulated by type-I interferon.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Nejad,Katherine A Pillman,Katherine J Siddle,Geneviève Pépin,Minna-Liisa Änkö,Claire E McCoy,Traude H Beilharz,Lluís Quintana-Murci,Gregory J Goodall,Cameron P Bracken,Michael P Gantier

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PNPT1 antibody

Immunoprecipitation - Anti-PNPT1 antibody (AB157109)

Western blot - Anti-PNPT1 antibody (AB157109)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise