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AB235599

Anti-PNRC2 antibody

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(1 Publication)

Rabbit Polyclonal PNRC2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PNRC2.

View Alternative Names

HSPC208, PNRC2, Proline-rich nuclear receptor coactivator 2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNRC2 antibody (AB235599)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNRC2 antibody (AB235599)

Paraffin-embedded human kidney tissue stained for PNRC2 using ab235599 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PNRC2 antibody (AB235599)
  • WB

Supplier Data

Western blot - Anti-PNRC2 antibody (AB235599)

All lanes:

Western blot - Anti-PNRC2 antibody (ab235599) at 1/500 dilution

All lanes:

U-251 MG (human brain glioma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PNRC2.

Q9NPJ4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PNRC2 protein also known as Proline-Rich Nuclear Receptor Coactivator 2 plays roles in the regulation of nuclear receptor activity. With an approximate mass of 15 kDa PNRC2 facilitates protein-protein interactions within the nucleus. This protein is expressed in various tissues indicating a widespread physiological influence. Researchers have identified it along with other proteins that contain proline-rich motifs across different cell types.
Biological function summary

PNRC2 interacts with nuclear receptors and transcriptional machinery to modulate gene expression. It acts as a coactivator meaning it enhances the activity of nuclear receptors though it does not bind to DNA directly. PNRC2 forms part of larger multi-protein complexes working in concert with transcription factors to regulate specific target genes. These interactions influence important biological processes including cell growth and apoptosis.

Pathways

PNRC2 impacts nuclear receptor signaling and gene transcription regulation. This protein involves itself in the steroid hormone receptor pathway and potentially the thyroid hormone receptor pathway. In these pathways PNRC2 interacts with proteins such as the Retinoid X Receptor (RXR) and the Estrogen Receptor (ER). These interactions allow PNRC2 to modulate cellular responses to hormones affecting processes such as cell proliferation and differentiation.

PNRC2 has connections to hormone-related cancers and metabolic disorders. In breast cancer PNRC2 influences the activity of the Estrogen Receptor an important player in tumor growth and progression. PNRC2 also displays associations with proteins like the Androgen Receptor in the context of prostate cancer. Dysregulation of PNRC2 activity could contribute to abnormal cell proliferation highlighting its potential as a therapeutic target in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in nonsense-mediated mRNA decay (NMD) by acting as a bridge between the mRNA decapping complex and the NMD machinery (PubMed : 19150429). May act by targeting the NMD machinery to the P-body and recruiting the decapping machinery to aberrant mRNAs (PubMed : 19150429). Required for UPF1/RENT1 localization to the P-body (PubMed : 19150429). Plays a role in glucocorticoid receptor-mediated mRNA degradation by interacting with the glucocorticoid receptor NR3C1 in a ligand-dependent manner when it is bound to the 5' UTR of target mRNAs and recruiting the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed : 25775514). Also acts as a nuclear receptor coactivator (PubMed : 11574675). May play a role in controlling the energy balance between energy storage and energy expenditure (By similarity).
See full target information PNRC2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:4872-4888 PubMed38412296

2024

RNA-RNA interactions between respiratory syncytial virus and miR-26 and miR-27 are associated with regulation of cell cycle and antiviral immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Ressel,Sujai Kumar,Jose Roberto Bermúdez-Barrientos,Katrina Gordon,Julia Lane,Jin Wu,Cei Abreu-Goodger,Jürgen Schwarze,Amy H Buck
View all publications
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Product promise

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