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AB250957

Anti-POC5 antibody [EPR14000-76] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal POC5 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C5orf37, POC5, Centrosomal protein POC5, Protein of centriole 5, hPOC5

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (AB250957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (AB250957)

This data was developed using ab188330, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling POC5 with ab188330 at 1/50 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (AB250957)
  • WB

Supplier Data

Western blot - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (AB250957)

This data was developed using ab188330, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-POC5 antibody [EPR14000-76] - N-terminal (<a href='/en-us/products/primary-antibodies/poc5-antibody-epr14000-76-n-terminal-ab188330'>ab188330</a>) at 1/1000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

Predicted band size: 63 kDa

Observed band size: 75 kDa

false

Western blot - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (AB250957)
  • WB

Supplier Data

Western blot - Anti-POC5 antibody [EPR14000-76] - BSA and Azide free (AB250957)

This data was developed using ab188330, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-POC5 antibody [EPR14000-76] - N-terminal (<a href='/en-us/products/primary-antibodies/poc5-antibody-epr14000-76-n-terminal-ab188330'>ab188330</a>) at 1/1000 dilution

All lanes:

Y79 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 63 kDa

Observed band size: 75 kDa

false

  • Unconjugated

    Anti-POC5 antibody [EPR14000-76] - N-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14000-76

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

ab250957 is the carrier-free version of ab188330.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POC5 also known as Proteome of Centriole 5 is a protein encoded by the POC5 gene. The POC5 protein has a molecular mass of about 63 kDa. It is expressed in various tissues with high expression levels noted in the testis and brain. POC5 localizes to the centrioles which are key structures within the centrosome involved in the formation of cilia and flagella essential for cellular movement and organization.
Biological function summary

POC5 contributes to the maintenance and function of centrioles. It forms part of a complex that stabilizes the structure of centrioles ensuring correct centrosome duplication and cycle progression. By maintaining centriole integrity POC5 supports proper cell division and ensures that chromosomal segregation proceeds without errors during mitosis preserving genomic stability.

Pathways

POC5 integrates into the centrosome duplication pathway and the regulation of the cell cycle. It interacts with other proteins including CEP63 and PLK4 to ensure robust centriole duplication and prevent errors during cell cycle phases. POC5 therefore has an essential role in centrosome-related pathways which govern critical aspects of cell division and replication.

POC5 has been linked to ciliopathies and certain cancers like retinal degeneration and colorectal cancer. In ciliopathies impaired POC5 function disrupts the normal formation and stability of cilia causing defects that can lead to disease. Connections with proteins such as CEP290 in retinal degeneration highlight POC5's involvement in maintaining ciliary function. Increased or altered expression of POC5 in cancer has been observed suggesting its potential role as an oncogenic factor through disrupting normal centrosome function and chromosomal integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for the assembly of the distal half of centrioles, required for centriole elongation.
See full target information POC5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes 14: PubMed37239471

2023

Differential Regulation of POC5 by ERα in Human Normal and Scoliotic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Amani Hassan,Edward T Bagu,Shunmoogum A Patten,Sirinart Molidperee,Stefan Parent,Soraya Barchi,Isabelle Villemure,André Tremblay,Florina Moldovan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com