AB109059

Anti-Podoplanin antibody

BOND RX™ Validated
What is this?

(1 Review)

(12 Publications)

Rabbit Polyclonal Podoplanin antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 12 publications.

View Alternative Names

GP36, PSEC0003, PSEC0025, PDPN, Podoplanin, Aggrus, Glycoprotein 36, PA2.26 antigen, T1-alpha, Gp36, T1A

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody (AB109059)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody (AB109059)

IHC image of Podoplanin staining in a section of formalin-fixed paraffin-embedded normal human lung performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab109059, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody (AB109059)

IHC image of Podoplanin staining in a section of formalin-fixed paraffin-embedded normal mouse lung performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab109059, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is designed to detect podoplanin, which is also referred to as gp38 (mouse and rat) and gp36 (human).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Podoplanin also known as gp36 and PDPN is a small transmembrane protein weighing approximately 36 kDa. It mainly expresses in lymphatic endothelial cells podocytes and various other tissues like the lung and kidney. It functions as a marker in mesothelial cells and is noticeable in histochemical studies such as mesothelioma using podoplanin staining in immunohistochemistry (IHC). Podoplanin emerges as an important tool in identifying lymphatic vessels and has significant roles in the development of certain tissues.

Biological function summary

The protein participates in maintaining the integrity of cell structures and lymphangiogenesis. This protein does not form part of a larger complex but actively interacts with different molecules. In lymphoid tissues podoplanin contributes to the proper formation of lymphatic channels and assists in platelet aggregation. These actions are pivotal in wound healing and protecting the epithelial cell layers in multiple organs.

Pathways

Podoplanin impacts the platelet activation pathway and the Lymphatic Vessel Development pathway. Its interaction with CLEC-2 (C-type lectin-like receptor 2) triggers downstream signaling leading to changes in platelet morphology and actin cytoskeleton reorganization. Podoplanin’s activation of these pathways links it to key processes that include cell migration and tissue homeostasis TGF-beta interaction also represents a significant relationship within these pathways.

Podoplanin has correlations with conditions like cancer specifically mesothelioma and squamous cell carcinoma. Dysregulation of podoplanin and its pathways may contribute to tumor progression and metastasis. In mesothelioma podoplanin interacts with other proteins like E-cadherin influencing cancer cell migration and adhesion. Its role in disease states highlights the importance of podoplanin IHC as a diagnostic tool aiding in the better understanding and identification of disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed : 14522983, PubMed : 15231832, PubMed : 17222411, PubMed : 17616532, PubMed : 18215137). Interaction with CD9, on the contrary, attenuates platelet aggregation induced by PDPN (PubMed : 18541721). Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness (PubMed : 17046996, PubMed : 21376833). Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (PubMed : 20962267). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (By similarity). Through binding with LGALS8 may participate in connection of the lymphatic endothelium to the surrounding extracellular matrix (PubMed : 19268462). In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed : 15515019). Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (PubMed : 25486435). Required for normal lung cell proliferation and alveolus formation at birth (By similarity). Does not function as a water channel or as a regulator of aquaporin-type water channels (PubMed : 9651190). Does not have any effect on folic acid or amino acid transport (By similarity).

See full target information PDPN

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Blood vessels, thrombosis & hemostasis 2:100034 PubMed40766876

2025

Podoplanin and microthrombi in lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jahnavi Gollamudi,Ricardo Gonzalez Delgado,Min Soon Cho,Brianne Wharton,Hani Lee,Animesh Vadaparti,Swapan K Dasgupta,Miguel A Cruz,Perumal Thiagarajan,Vahid Afshar-Kharghan

Nature communications 16:829 PubMed39827193

2025

Lymphatic platelet thrombosis limits bone repair by precluding lymphatic transporting DAMPs.

Applications

Unspecified application

Species

Unspecified reactive species

Yangkang Zheng,Lin Cong,Li Zhao,Pengyu Wang,Lianping Xing,Junling Liu,Hao Xu,Ning Li,Yongjian Zhao,Luying Yuan,Qi Shi,Xueqing Sun,Qianqian Liang,YongJun Wang

OTO open 8:e171 PubMed39050361

2024

Lymphatic Vessels in the Inner Ear of Patients With Meniere Disease: A Novel Pathological Finding.

Applications

Unspecified application

Species

Unspecified reactive species

Daogong Zhang,Xiaofei Li,Yafeng Lv,Yongdong Song,Ligang Kong,Boqin Li,Jinfeng Zheng,Nicolas Pérez-Fernández,Zhaomin Fan,Haibo Wang

Frontiers in physiology 13:1069466 PubMed36699692

2023

Impact of KvLQT1 potassium channel modulation on alveolar fluid homeostasis in an animal model of thiourea-induced lung edema.

Applications

Unspecified application

Species

Unspecified reactive species

Mélissa Aubin Vega,Alban Girault,Damien Adam,Jasmine Chebli,Anik Privé,Émilie Maillé,Annette Robichaud,Emmanuelle Brochiero

Journal of microscopy and ultrastructure 12:120-125 PubMed39507645

2022

Utility of a Highly Specific and Sensitive Podoplanin/D2-40, Calretinin, Thyroid Transcription Factor-1, and Carcinoembryonic Antigen/CD66e Immunohistochemical Panel in Differentiating Malignant Pleural Mesothelioma from Metastatic Adenocarcinoma: An Egyptian Experience.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha A Khairy,Eman Khaled,Samar El Sheikh,Ahmed Abdlaziz,Sara E Khalifa

Nature communications 13:3155 PubMed35672321

2022

Viral coinfection promotes tuberculosis immunopathogenesis by type I IFN signaling-dependent impediment of Th1 cell pulmonary influx.

Applications

Unspecified application

Species

Unspecified reactive species

Tae Gun Kang,Kee Woong Kwon,Kyungsoo Kim,Insuk Lee,Myeong Joon Kim,Sang-Jun Ha,Sung Jae Shin

Frontiers in immunology 13:853769 PubMed35572508

2022

Microvesicle-Mediated Communication Within the Alveolar Space: Mechanisms of Uptake by Epithelial Cells and Alveolar Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Sanooj Soni,Kieran P O'Dea,Eiko Abe,Maryam Khamdan,Sneh V Shah,Padmini Sarathchandra,Michael R Wilson,Masao Takata

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22248 PubMed35239213

2022

Tumor microenvironment remodeling modulates macrophage phenotype in breast cancer lymphangiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Du,Manlin Cao,Yiwen Liu,Yiqing He,Cuixia Yang,Guoliang Zhang,Youben Fan,Feng Gao

bioRxiv : the preprint server for biology : PubMed32935109

2020

Age-dependent regulation of SARS-CoV-2 cell entry genes and cell death programs correlates with COVID-19 disease severity.

Applications

Unspecified application

Species

Unspecified reactive species

Zintis Inde,Clarence Yapp,Gaurav N Joshi,Johan Spetz,Cameron Fraser,Brian Deskin,Elisa Ghelfi,Chhinder Sodhi,David J Hackam,Lester Kobzik,Ben A Croker,Douglas Brownfield,Hongpeng Jia,Kristopher A Sarosiek

Cancer cell 38:212-228.e13 PubMed32707078

2020

Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsay M LaFave,Vinay K Kartha,Sai Ma,Kevin Meli,Isabella Del Priore,Caleb Lareau,Santiago Naranjo,Peter M K Westcott,Fabiana M Duarte,Venkat Sankar,Zachary Chiang,Alison Brack,Travis Law,Haley Hauck,Annalisa Okimoto,Aviv Regev,Jason D Buenrostro,Tyler Jacks

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Podoplanin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody (AB109059)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody (AB109059)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise