Mouse Monoclonal Podoplanin antibody. Carrier free. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDPN aa 1-150.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PDPN/1433] - BSA and Azide free (AB234036), expandable thumbnail](https://content.abcam.com/products/images/podoplanin-antibody-pdpn-1433-bsa-and-azide-free-ab234036--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124681.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PDPN/1433] - BSA and Azide free (AB234036)")
![Protein Array - Anti-Podoplanin antibody [PDPN/1433] - BSA and Azide free (AB234036), expandable thumbnail](https://content.abcam.com/products/images/podoplanin-antibody-pdpn-1433-bsa-and-azide-free-ab234036--protein-array-img1259.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Podoplanin antibody [PDPN/1433] - BSA and Azide free (AB234036)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human PDPN aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q86YL7
Reactivity data
Select an application| IHC-P | Protein Array | |
|---|---|---|
| Human | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
1 product for Alternative Version
Target data
Function
Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed:14522983, PubMed:15231832, PubMed:17222411, PubMed:17616532, PubMed:18215137). Interaction with CD9, on the contrary, attenuates platelet aggregation induced by PDPN (PubMed:18541721). Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness (PubMed:17046996, PubMed:21376833). Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (PubMed:20962267). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (By similarity). Through binding with LGALS8 may participate in connection of the lymphatic endothelium to the surrounding extracellular matrix (PubMed:19268462). In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed:15515019). Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (PubMed:25486435). Required for normal lung cell proliferation and alveolus formation at birth (By similarity). Does not function as a water channel or as a regulator of aquaporin-type water channels (PubMed:9651190). Does not have any effect on folic acid or amino acid transport (By similarity).
Alternative names
GP36, PSEC0003, PSEC0025, PDPN, Podoplanin, Aggrus, Glycoprotein 36, PA2.26 antigen, T1-alpha, Gp36, T1A
Recommended products
Mouse Monoclonal Podoplanin antibody. Carrier free. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDPN aa 1-150.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human PDPN aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q86YL7
- Carrier free
- Yes
- Clone number
- PDPN/1433
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
ab234036 is purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Podoplanin also known as gp36 and PDPN is a small transmembrane protein weighing approximately 36 kDa. It mainly expresses in lymphatic endothelial cells podocytes and various other tissues like the lung and kidney. It functions as a marker in mesothelial cells and is noticeable in histochemical studies such as mesothelioma using podoplanin staining in immunohistochemistry (IHC). Podoplanin emerges as an important tool in identifying lymphatic vessels and has significant roles in the development of certain tissues.
- Biological function summary
The protein participates in maintaining the integrity of cell structures and lymphangiogenesis. This protein does not form part of a larger complex but actively interacts with different molecules. In lymphoid tissues podoplanin contributes to the proper formation of lymphatic channels and assists in platelet aggregation. These actions are pivotal in wound healing and protecting the epithelial cell layers in multiple organs.
- Pathways
Podoplanin impacts the platelet activation pathway and the Lymphatic Vessel Development pathway. Its interaction with CLEC-2 (C-type lectin-like receptor 2) triggers downstream signaling leading to changes in platelet morphology and actin cytoskeleton reorganization. Podoplanin’s activation of these pathways links it to key processes that include cell migration and tissue homeostasis TGF-beta interaction also represents a significant relationship within these pathways.
- Associated diseases and disorders
Podoplanin has correlations with conditions like cancer specifically mesothelioma and squamous cell carcinoma. Dysregulation of podoplanin and its pathways may contribute to tumor progression and metastasis. In mesothelioma podoplanin interacts with other proteins like E-cadherin influencing cancer cell migration and adhesion. Its role in disease states highlights the importance of podoplanin IHC as a diagnostic tool aiding in the better understanding and identification of disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PDPN/1433] - BSA and Azide free (ab234036), expandable thumbnail](https://content.abcam.com/products/images/podoplanin-antibody-pdpn-1433-bsa-and-azide-free-ab234036--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124681.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PDPN/1433] - BSA and Azide free (ab234036)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PDPN/1433] - BSA and Azide free (ab234036)
This IHC-P data was generated using the same anti-Podoplanin/gp36 antibody clone PDPN/1433 in a different buffer formulation (cat# Anti-Podoplanin antibody [PDPN/1433] ab217886)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cervix tissue labeling Podoplanin with Anti-Podoplanin antibody [PDPN/1433] ab217886 at 1 μg/mL.
![Protein Array - Anti-Podoplanin antibody [PDPN/1433] - BSA and Azide free (ab234036), expandable thumbnail](https://content.abcam.com/products/images/podoplanin-antibody-pdpn-1433-bsa-and-azide-free-ab234036--protein-array-img1259.jpg "Protein Array - Anti-Podoplanin antibody [PDPN/1433] - BSA and Azide free (ab234036)")
Protein Array - Anti-Podoplanin antibody [PDPN/1433] - BSA and Azide free (ab234036)
This data was produced with Anti-Podoplanin antibody [PDPN/1433] ab217886, the same antibody in a different formulation with BSA and Azide.
Anti-Podoplanin antibody [PDPN/1433] ab217886 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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