AB256559

Anti-Podoplanin antibody [PMab-1]

Recombinant
What is this?

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(18 Publications)

Anti-Podoplanin antibody [PMab-1] (ab256559) is a rat monoclonal antibody detecting Podoplanin in Western Blot, IHC-P, IHC-Fr. Suitable for Mouse.

View Alternative Names

Gp38, Ots8, Pdpn, Podoplanin, Glycoprotein 38, OTS-8, PA2.26 antigen, Transmembrane glycoprotein E11, E11

5 Images

Immunohistochemistry (Frozen sections) - Anti-Podoplanin antibody [PMab-1] (AB256559)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Podoplanin antibody [PMab-1] (AB256559)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Podoplanin with ab256559 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PMab-1] (AB256559)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PMab-1] (AB256559)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Podoplanin with ab256559 at 1/1000 dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the podocytes in the mouse kidney is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Podoplanin antibody [PMab-1] (AB256559)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Podoplanin with ab256559 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PMab-1] (AB256559)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Podoplanin with ab256559 at 1/1000 dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse lung is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Lab

Western blot - Anti-Podoplanin antibody [PMab-1] (AB256559)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time 26 secs.

All lanes:

Western blot - Anti-Podoplanin antibody [PMab-1] (ab256559) at 1/1000 dilution

All lanes:

mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 16 kDa

false

Carrier free

Anti-Podoplanin antibody [PMab-1] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Podoplanin antibody [PMab-1]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

PMab-1

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

WB, IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-Podoplanin antibody [PMab-1] (ab256559) is a rat recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse samples.

What is the molecular weight of Podoplanin?
Anti-Podoplanin [PMab-1] (ab256559) specifically detects a band for Podoplanin (UniProt: Q62011) at a molecular weight of 16kDa.

Other related products
We have a range of other formats of antibody clone [PMab-1] also available for your convenience: ab256559, Carrier free - ab256564, Alexa Fluor® 488 - ab318248

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Podoplanin also known as gp36 and PDPN is a small transmembrane protein weighing approximately 36 kDa. It mainly expresses in lymphatic endothelial cells podocytes and various other tissues like the lung and kidney. It functions as a marker in mesothelial cells and is noticeable in histochemical studies such as mesothelioma using podoplanin staining in immunohistochemistry (IHC). Podoplanin emerges as an important tool in identifying lymphatic vessels and has significant roles in the development of certain tissues.

Biological function summary

The protein participates in maintaining the integrity of cell structures and lymphangiogenesis. This protein does not form part of a larger complex but actively interacts with different molecules. In lymphoid tissues podoplanin contributes to the proper formation of lymphatic channels and assists in platelet aggregation. These actions are pivotal in wound healing and protecting the epithelial cell layers in multiple organs.

Pathways

Podoplanin impacts the platelet activation pathway and the Lymphatic Vessel Development pathway. Its interaction with CLEC-2 (C-type lectin-like receptor 2) triggers downstream signaling leading to changes in platelet morphology and actin cytoskeleton reorganization. Podoplanin’s activation of these pathways links it to key processes that include cell migration and tissue homeostasis TGF-beta interaction also represents a significant relationship within these pathways.

Podoplanin has correlations with conditions like cancer specifically mesothelioma and squamous cell carcinoma. Dysregulation of podoplanin and its pathways may contribute to tumor progression and metastasis. In mesothelioma podoplanin interacts with other proteins like E-cadherin influencing cancer cell migration and adhesion. Its role in disease states highlights the importance of podoplanin IHC as a diagnostic tool aiding in the better understanding and identification of disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed : 14522983, PubMed : 15231832, PubMed : 17616532, PubMed : 20110424). Interaction with CD9, on the contrary, attenuates platelet aggregation and pulmonary metastasis induced by PDPN. Mediates effects on cell migration and adhesion through its different partners. Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness. Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (By similarity). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (PubMed : 25347465). Through binding with LGALS8 may participate in connection of the lymphatic endothelium to the surrounding extracellular matrix (By similarity). In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed : 10574709). Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (By similarity). Required for normal lung cell proliferation and alveolus formation at birth (PubMed : 12654292). Does not function as a water channel or as a regulator of aquaporin-type water channels (By similarity). Does not have any effect on folic acid or amino acid transport (PubMed : 12032185).

See full target information Pdpn

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 44:2689-2714 PubMed40394416

2025

Erk1 is an oncoprotein that causes hepatocellular carcinoma in mice and imposes a rigorous negative feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine Soudah,Alexey Baskin,Merav Darash-Yahana,Ilona Darlyuk-Saadon,Karina Smorodinsky-Atias,Tali Shalit,Wei-Ping Yu,Alon Savidor,Eli Pikarsky,David Engelberg

Journal of translational medicine 23:432 PubMed40217301

2025

Tumor cell-derived osteopontin promotes tumor fibrosis indirectly via tumor-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yuying Tan,Yong-Guang Yang,Xiaoying Zhang,Lei Zhao,Xiaocong Wang,Wentao Liu

JCI insight 10: PubMed39903516

2025

Molecular control of PDPNhi macrophage subset induction by ADAP as a host defense in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Pengchao Zhang,Xinning Wang,Xiaodong Yang,Hebin Liu

Cell discovery 10:109 PubMed39468013

2024

Targeting a chemo-induced adaptive signaling circuit confers therapeutic vulnerabilities in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yohei Saito,Yi Xiao,Jun Yao,Yunhai Li,Wendao Liu,Arseniy E Yuzhalin,Yueh-Ming Shyu,Hongzhong Li,Xiangliang Yuan,Ping Li,Qingling Zhang,Ziyi Li,Yongkun Wei,Xuedong Yin,Jun Zhao,Seyed M Kariminia,Yao-Chung Wu,Jinyang Wang,Jun Yang,Weiya Xia,Yutong Sun,Eek-Hoon Jho,Paul J Chiao,Rosa F Hwang,Haoqiang Ying,Huamin Wang,Zhongming Zhao,Anirban Maitra,Mien-Chie Hung,Ronald A DePinho,Dihua Yu

Cell death & disease 15:640 PubMed39251573

2024

Mesenchymal stromal cells restrain the Th17 cell response via L-amino-acid oxidase within lymph nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Ni,Le Zhen,Zhu Zeng,Jingwen Yang,Yukai Wang,Huanke Xu,Qixiang Zhang,Yongcheng Zhu,Yu Tao,Jing Wang,Qing Liu,Kezheng Yi,Yang Chen,Qian Chen,Guangji Wang,Fang Zhou,Yunlong Shan

Redox biology 74:103231 PubMed38861835

2024

Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yu,Yu Fu,Jiameng Gao,Qingqing Zhang,Nan Zhang,Zhiyuan Zhang,Xuemei Jiang,Chang Chen,Zongmei Wen

Nature communications 15:1496 PubMed38383468

2024

Exosomes define a local and systemic communication network in healthy pancreas and pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bárbara Adem,Nuno Bastos,Carolina F Ruivo,Sara Sousa-Alves,Carolina Dias,Patrícia F Vieira,Inês A Batista,Bruno Cavadas,Dieter Saur,José C Machado,Dawen Cai,Sonia A Melo

Cell death & disease 14:708 PubMed37903800

2023

The microprotein encoded by exosomal lncAKR1C2 promotes gastric cancer lymph node metastasis by regulating fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Gan Zhu,Jiayu Yang,Yuehong Zhu,Qihang Zhu,Wen Pan,Siyu Deng,Yi He,Duo Zuo,Peiyun Wang,Yueting Han,Hai-Yang Zhang

iScience 26:108010 PubMed37829206

2023

Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Kameyama,Muneaki Miyata,Hajime Shiotani,Jun Adachi,Soichiro Kakuta,Yasuo Uchiyama,Kiyohito Mizutani,Yoshimi Takai

Journal of nanobiotechnology 21:295 PubMed37626408

2023

Human umbilical cord mesenchymal stromal cell small extracellular vesicle transfer of microRNA-223-3p to lung epithelial cells attenuates inflammation in acute lung injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Shiyang Ma,Baihua Luo,Haojie Hao,Yanqin Li,Hang Yang,Fei Zhu,Peipei Zhang,Ruichao Niu,Pinhua Pan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Podoplanin antibody [PMab-1]

Immunohistochemistry (Frozen sections) - Anti-Podoplanin antibody [PMab-1] (AB256559)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PMab-1] (AB256559)

Immunohistochemistry (Frozen sections) - Anti-Podoplanin antibody [PMab-1] (AB256559)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PMab-1] (AB256559)

Western blot - Anti-Podoplanin antibody [PMab-1] (AB256559)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise