JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB256564

Anti-Podoplanin antibody [PMab-1] - BSA and Azide free

4

(1 Review)

|

(4 Publications)

Rat Recombinant Monoclonal Podoplanin antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

Gp38, Ots8, Pdpn, Podoplanin, Glycoprotein 38, OTS-8, PA2.26 antigen, Transmembrane glycoprotein E11, E11

5 Images
Immunohistochemistry (Frozen sections) - Anti-Podoplanin antibody [PMab-1] - BSA and Azide free (AB256564)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Podoplanin antibody [PMab-1] - BSA and Azide free (AB256564)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Podoplanin with ab256559 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PMab-1] - BSA and Azide free (AB256564)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PMab-1] - BSA and Azide free (AB256564)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Podoplanin / gp36 with ab256559 at 1/1000 dilution followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the podocytes in the mouse kidney is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

Immunohistochemistry (Frozen sections) - Anti-Podoplanin antibody [PMab-1] - BSA and Azide free (AB256564)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Podoplanin antibody [PMab-1] - BSA and Azide free (AB256564)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Podoplanin with ab256559 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PMab-1] - BSA and Azide free (AB256564)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin antibody [PMab-1] - BSA and Azide free (AB256564)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Podoplanin / gp36 with ab256559 at 1/1000 dilution followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse lung is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

Western blot - Anti-Podoplanin antibody [PMab-1] - BSA and Azide free (AB256564)
  • WB

Lab

Western blot - Anti-Podoplanin antibody [PMab-1] - BSA and Azide free (AB256564)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time 26 secs.

This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

All lanes:

Western blot - Anti-Podoplanin antibody [PMab-1] (<a href='/en-us/products/primary-antibodies/podoplanin-antibody-pmab-1-ab256559'>ab256559</a>) at 1/1000 dilution

All lanes:

mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 16 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

PMab-1

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse

Applications

WB, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB323923

Human Podoplanin ELISA Kit- Extracellular

SimpleStep|Recombinant

Sandwich ELISA - Human Podoplanin ELISA Kit- Extracellular (AB323923)

  • 0
  • 0
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB33682

Anti-LYVE1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (AB33682)

  • 4
  • 15
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB182981

Anti-CD31 antibody [EPR17259]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] (AB182981)

  • 5
  • 22
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
style
left-column

Product details

ab256564 is the carrier-free version of ab256559.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Podoplanin also known as gp36 and PDPN is a small transmembrane protein weighing approximately 36 kDa. It mainly expresses in lymphatic endothelial cells podocytes and various other tissues like the lung and kidney. It functions as a marker in mesothelial cells and is noticeable in histochemical studies such as mesothelioma using podoplanin staining in immunohistochemistry (IHC). Podoplanin emerges as an important tool in identifying lymphatic vessels and has significant roles in the development of certain tissues.
Biological function summary

The protein participates in maintaining the integrity of cell structures and lymphangiogenesis. This protein does not form part of a larger complex but actively interacts with different molecules. In lymphoid tissues podoplanin contributes to the proper formation of lymphatic channels and assists in platelet aggregation. These actions are pivotal in wound healing and protecting the epithelial cell layers in multiple organs.

Pathways

Podoplanin impacts the platelet activation pathway and the Lymphatic Vessel Development pathway. Its interaction with CLEC-2 (C-type lectin-like receptor 2) triggers downstream signaling leading to changes in platelet morphology and actin cytoskeleton reorganization. Podoplanin’s activation of these pathways links it to key processes that include cell migration and tissue homeostasis TGF-beta interaction also represents a significant relationship within these pathways.

Podoplanin has correlations with conditions like cancer specifically mesothelioma and squamous cell carcinoma. Dysregulation of podoplanin and its pathways may contribute to tumor progression and metastasis. In mesothelioma podoplanin interacts with other proteins like E-cadherin influencing cancer cell migration and adhesion. Its role in disease states highlights the importance of podoplanin IHC as a diagnostic tool aiding in the better understanding and identification of disease pathology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed : 14522983, PubMed : 15231832, PubMed : 17616532, PubMed : 20110424). Interaction with CD9, on the contrary, attenuates platelet aggregation and pulmonary metastasis induced by PDPN. Mediates effects on cell migration and adhesion through its different partners. Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness. Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (By similarity). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (PubMed : 25347465). Through binding with LGALS8 may participate in connection of the lymphatic endothelium to the surrounding extracellular matrix (By similarity). In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed : 10574709). Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (By similarity). Required for normal lung cell proliferation and alveolus formation at birth (PubMed : 12654292). Does not function as a water channel or as a regulator of aquaporin-type water channels (By similarity). Does not have any effect on folic acid or amino acid transport (PubMed : 12032185).
See full target information Pdpn
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 15:52-56 PubMed29998193

2018

Epitope mapping of anti-mouse podoplanin monoclonal antibody PMab-1.

Applications

Unspecified application

Species

Unspecified reactive species

Shinji Yamada,Shunsuke Itai,Mika K Kaneko,Satoru Konnai,Yukinari Kato

Monoclonal antibodies in immunodiagnosis and immun 36:77-79 PubMed28387612

2017

Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin.

Applications

Unspecified application

Species

Unspecified reactive species

Shinji Yamada,Mika K Kaneko,Takuro Nakamura,Osamu Ichii,Satoru Konnai,Yukinari Kato

Investigative ophthalmology & visual science 55:4813-22 PubMed24985477

2014

The effect of podoplanin inhibition on lymphangiogenesis under pathological conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Yuko Maruyama,Kazuichi Maruyama,Yukinari Kato,Kentaro Kajiya,Satoru Moritoh,Kotaro Yamamoto,Yuko Matsumoto,Mika Sawane,Dontscho Kerjaschki,Toru Nakazawa,Shigeru Kinoshita

Acta histochemica et cytochemica 45:227-37 PubMed23012488

2012

Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin.

Applications

Unspecified application

Species

Unspecified reactive species

Chiaki Kaji,Yuta Tsujimoto,Mika Kato Kaneko,Yukinari Kato,Yoshihiko Sawa
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com