Anti-PODXL antibody [EPR30479-53]

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Flow Cytometry - Anti-PODXL antibody [EPR30479-53] (AB327011)

Flow cytometric analysis of cells labelling PODXL with ab327011 at 0.2ug/mL compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 488, ab150077) at 1/4000 dilution was used as the secondary antibody.

Flow cytometry overlay histogram showing left bEnd.3 positive cells, middle Neuro-2a positive cells, and right negative NIH/3T3 stained with ab327011 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein–protein interaction, followed by the antibody (ab327011) (1 × 10⁶ in 100 µl at 0.2 µg/ml (1/2500)) for 30 min on ice. The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30 min on ice. Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] – Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50 mW blue laser (488 nm) and 525/40 bandpass filter.