AB205350

Anti-PODXL antibody - N-terminal

Name: Anti-PODXL antibody - N-terminal (ab205350) | Abcam
Brand: Abcam Limited
SKU: AB205350
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal PODXL antibody. N-terminal. Suitable for WB and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PODXL.

View Alternative Names

PCLP, PCLP1, PODXL, Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1

1 Images

Supplier Data

Western blot - Anti-PODXL antibody - N-terminal (AB205350)

All lanes:

Western blot - Anti-PODXL antibody - N-terminal (ab205350) at 1/500 dilution

All lanes:

rat kidney lysate

Predicted band size: 58 kDa

Observed band size: 73 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Human PODXL. The exact immunogen used to generate this antibody is proprietary information.

O00592

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PODXL also known as podocalyxin-like protein is a type I transmembrane glycoprotein possessing a mass of approximately 165 kDa. This protein is a member of the CD34 family and exhibits expression primarily on the apical surface of podocytes in the kidney most hematopoietic stem cells and certain cancer cell types. In its mechanical role PODXL mainly functions in cell adhesion and the maintenance of cell shape through its cytoplasmic domain that connects with the cytoskeleton. The sialylated and sulfated glycosaminoglycan chains on the extracellular domain contribute to its charge and barrier functions.

Biological function summary

The primary function of podocalyxin-like protein revolves around maintaining the filtration barrier in the kidney by repelling negatively charged molecules. It forms part of the slit diaphragm complex along with proteins like nephrin which provides the structural support necessary for its function. PODXL also plays roles in the development of the vascular system and hematopoietic cell lineage contributing to cellular processes like migration and proliferation. Its interaction with the actin cytoskeleton ensures proper localization and function within these biological contexts.

Pathways

There are important connections between podocalyxin-like protein and the Ras signaling pathway involved in cell growth and adhesion processes. It can also engage in integrin-mediated cell adhesion pathways facilitating processes such as cell signaling and migration. Through these pathways it associates with proteins like ezrin which links it to the cytoskeleton and contributes to its role in cell morphology and motility.

Altered PODXL expression shows relevance in aggressive forms of cancer including renal cell carcinoma and breast cancer. The protein associates with different markers of poor prognosis making it of interest in cancer research. Furthermore in kidney diseases such as focal segmental glomerulosclerosis abnormal PODXL expression affects the slit diaphragm's function leading to proteinuria. Its interaction with nephrin can highlight disrupted cellular architecture typical of these disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

See full target information PODXL

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 41:2546-2560 PubMed33818185

2021

A large portion of the astrocyte proteome is dedicated to perivascular endfeet, including critical components of the electron transport chain.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse A Stokum,Bosung Shim,Weiliang Huang,Maureen Kane,Jesse A Smith,Volodymyr Gerzanich,J Marc Simard

Biochemical pharmacology 166:139-152 PubMed31085161

2019

Magnesium isoglycyrrhizinate ameliorates fructose-induced podocyte apoptosis through downregulation of miR-193a to increase WT1.

Applications

Unspecified application

Species

Unspecified reactive species

Tu-Shuai Li,Li Chen,Shan-Chun Wang,Yan-Zi Yang,Hong-Jiang Xu,Hong-Mei Gu,Xiao-Juan Zhao,Ping Dong,Ying Pan,Zhan-Qiang Shang,Xi-Quan Zhang,Ling-Dong Kong

Cancer science 110:118-134 PubMed30407695

2018

Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoming Zhi,Huo Chen,Fei Liu,Ye Han,Daiwei Wan,Zhihua Xu,Yuting Kuang,Jin Zhou

Molecular medicine reports 14:681-8 PubMed27222353

2016

Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction.

Applications

IHC-P

Species

Rat

Qi Zhao,Yansheng Xue,Yi Yang,Zhibin Niu,Changlin Wang,Ying Hou,Hui Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PODXL antibody - N-terminal

Western blot - Anti-PODXL antibody - N-terminal (AB205350)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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