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Rabbit Polyclonal Pofut1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GDP-fucose protein O-fucosyltransferase 1 aa 1-250.

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Images

Western blot - Anti-Pofut1 antibody (AB154051), expandable thumbnail
  • Western blot - Anti-Pofut1 antibody (AB154051), expandable thumbnail
  • Western blot - Anti-Pofut1 antibody (AB154051), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Pofut1 antibody (AB154051), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human GDP-fucose protein O-fucosyltransferase 1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H488

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Predicted
Predicted
Cow
Predicted
Predicted
Dog
Predicted
Predicted
Pig
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/1000.00000 - 1/10000.00000

Notes

-

Species

Human

Dilution info

1/1000.00000 - 1/10000.00000

Notes

-

Predicted
Predicted

Species

Rat, Cow, Dog, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Dog, Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).

Alternative names

Recommended products

Rabbit Polyclonal Pofut1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GDP-fucose protein O-fucosyltransferase 1 aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human GDP-fucose protein O-fucosyltransferase 1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H488
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein known as Pofut1 or protein O-fucosyltransferase 1 functions mechanically by adding fucose a sugar molecule to serine or threonine residues on proteins specifically within the epidermal growth factor-like (EGF-like) repeats of substrates. Pofut1 has a molecular weight of approximately 50 kDa. This protein is expressed in various tissues including heart liver and brain indicating its significant role across different biological systems. Researchers commonly refer to Pofut1 in studies focused on protein glycosylation processes.

Biological function summary

Pofut1 is responsible for the proper folding and functioning of specific proteins that undergo fucosylation. It does not technically form part of a multi-protein complex but interacts tightly with substrates it modifies. Through its enzymatic action Pofut1 influences essential biological activities in cell communication and signaling. This glycosyltransferase also plays a pivotal role in modulating Notch signaling a critical pathway in the development and tissue regeneration processes.

Pathways

The significance of Pofut1 is most notable within the Notch signaling pathway where it modifies the Notch receptor enabling effective ligand-receptor interactions. Dysregulation of this pathway can lead to critical developmental defects. Pofut1 also connects to other pathways such as the Fringe-mediated glycosylation pathway. In this context Pofut1-related proteins like Fringe glycosyltransferases operate to further influence Notch signaling outcomes through additional glycosylation processing.

Associated diseases and disorders

The functionality of Pofut1 has links to specific syndromes and malignancies. For instance aberrations in its activity are associated with congenital heart defects due to disrupted Notch signaling affecting cardiac development. Additionally overexpression or mutations in Pofut1 relate to cancer as improper Notch signaling can lead to tumor progression. Relatedly proteins like Notch1 serve as intermediaries linking Pofut1 activity to such conditions thereby highlighting Pofut1's involvement in disease etiologies.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-Pofut1 antibody (ab154051), expandable thumbnail

    Western blot - Anti-Pofut1 antibody (ab154051)

    12% SDS PAGE

    All lanes: Western blot - Anti-Pofut1 antibody (ab154051) at 1/1000 dilution

    All lanes: HCT116 whole cell lysate at 30 µg

    Predicted band size: 44 kDa

  • Western blot - Anti-Pofut1 antibody (ab154051), expandable thumbnail

    Western blot - Anti-Pofut1 antibody (ab154051)

    10% SDS PAGE

    All lanes: Western blot - Anti-Pofut1 antibody (ab154051) at 1/1000 dilution

    All lanes: Mouse liver whole cell lysate at 50 µg

    Predicted band size: 44 kDa

  • Western blot - Anti-Pofut1 antibody (ab154051), expandable thumbnail

    Western blot - Anti-Pofut1 antibody (ab154051)

    12% SDS PAGE

    All lanes: Western blot - Anti-Pofut1 antibody (ab154051) at 1/5000 dilution

    All lanes: HUVEC whole cell lysate at 15 µg

    Predicted band size: 44 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Pofut1 antibody (ab154051), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Pofut1 antibody (ab154051)

    Immunofluorescent analysis of methanol-fixed HCT116 cells, labeling Pofut1 with ab154051 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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