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AB74302

Anti-Pofut1 antibody

4

(1 Review)

|

(3 Publications)

Rabbit Polyclonal Pofut1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human POFUT1.

View Alternative Names

FUT12, KIAA0180, POFUT1, GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1, O-FucT-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pofut1 antibody (AB74302)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pofut1 antibody (AB74302)

ab74302, at a 1/50 dilution, staining Pofut1 in paraffin embedded human lung carcinoma tissue in the absence (left image) and presence (right image) of the immunising peptide.

Western blot - Anti-Pofut1 antibody (AB74302)
  • WB

Unknown

Western blot - Anti-Pofut1 antibody (AB74302)

All lanes:

Western blot - Anti-Pofut1 antibody (ab74302) at 1/500 dilution

Lane 1:

extracts from HeLa cells at 5 µg

Lane 2:

extracts from Jurkat cells at 5 µg

Lane 3:

extracts from HepG2 cells at 5 µg

Lane 4:

extracts from Jurkat cells at 5 µg with immunising peptide

Predicted band size: 44 kDa

Observed band size: 117 kDa,22 kDa,28 kDa,30 kDa,44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human POFUT1. The exact immunogen used to generate this antibody is proprietary information.

Q9H488

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as Pofut1 or protein O-fucosyltransferase 1 functions mechanically by adding fucose a sugar molecule to serine or threonine residues on proteins specifically within the epidermal growth factor-like (EGF-like) repeats of substrates. Pofut1 has a molecular weight of approximately 50 kDa. This protein is expressed in various tissues including heart liver and brain indicating its significant role across different biological systems. Researchers commonly refer to Pofut1 in studies focused on protein glycosylation processes.
Biological function summary

Pofut1 is responsible for the proper folding and functioning of specific proteins that undergo fucosylation. It does not technically form part of a multi-protein complex but interacts tightly with substrates it modifies. Through its enzymatic action Pofut1 influences essential biological activities in cell communication and signaling. This glycosyltransferase also plays a pivotal role in modulating Notch signaling a critical pathway in the development and tissue regeneration processes.

Pathways

The significance of Pofut1 is most notable within the Notch signaling pathway where it modifies the Notch receptor enabling effective ligand-receptor interactions. Dysregulation of this pathway can lead to critical developmental defects. Pofut1 also connects to other pathways such as the Fringe-mediated glycosylation pathway. In this context Pofut1-related proteins like Fringe glycosyltransferases operate to further influence Notch signaling outcomes through additional glycosylation processing.

The functionality of Pofut1 has links to specific syndromes and malignancies. For instance aberrations in its activity are associated with congenital heart defects due to disrupted Notch signaling affecting cardiac development. Additionally overexpression or mutations in Pofut1 relate to cancer as improper Notch signaling can lead to tumor progression. Relatedly proteins like Notch1 serve as intermediaries linking Pofut1 activity to such conditions thereby highlighting Pofut1's involvement in disease etiologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).
See full target information POFUT1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 16:747-768 PubMed36852302

2023

Fucosyltransferases Regulated by Fusobacterium Nucleatum and Act as Novel Biomarkers in Colon Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Wang,Xuxu Liu,Jingjing Yu,Ziang Meng,Zhenyi Lv,Ce Shang,Qi Geng,Dawei Wang,Dongbo Xue,Long Li

Translational oncology 14:100900 PubMed33099185

2020

POFUT1 mRNA expression as an independent prognostic parameter in muscle-invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Wahby,Jonas Jarczyk,Alexander Fierek,Jakob Heinkele,Cleo-Aron Weis,Markus Eckstein,Thomas Martini,Stefan Porubsky,Mathias Hafner,Philipp Erben

Cancers 10: PubMed30380753

2018

as a Promising Novel Biomarker of Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Chabanais,François Labrousse,Alain Chaunavel,Agnès Germot,Abderrahman Maftah
View all publications
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Product promise

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