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AB272623

Anti-POGLUT1 antibody

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Rabbit Polyclonal POGLUT1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human POGLUT1 aa 300 to C-terminus.

View Alternative Names

C3orf9, CLP46, KTELC1, MDSRP, MDS010, UNQ490/PRO1006, POGLUT1, Protein O-glucosyltransferase 1, CAP10-like 46 kDa protein, KTEL motif-containing protein 1, Myelodysplastic syndromes relative protein, O-glucosyltransferase Rumi homolog, Protein O-xylosyltransferase POGLUT1, hCLP46, hRumi

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGLUT1 antibody (AB272623)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGLUT1 antibody (AB272623)

Paraffin-embedded formalin-fixed Human placenta tissue stained for POGLUT1 using ab272623 at 1/1000 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGLUT1 antibody (AB272623)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGLUT1 antibody (AB272623)

Paraffin-embedded formalin-fixed Human kidney tissue stained for POGLUT1 using ab272623 at 1/1000 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGLUT1 antibody (AB272623)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGLUT1 antibody (AB272623)

Paraffin-embedded formalin-fixed Human liver tissue stained for POGLUT1 using ab272623 at 1/1000 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGLUT1 antibody (AB272623)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGLUT1 antibody (AB272623)

Paraffin-embedded formalin-fixed Human tonsil tissue stained for POGLUT1 using ab272623 at 1/1000 in immunohistochemical analysis.

Western blot - Anti-POGLUT1 antibody (AB272623)
  • WB

Unknown

Western blot - Anti-POGLUT1 antibody (AB272623)

All lanes:

Western blot - Anti-POGLUT1 antibody (ab272623) at 0.4 µg/mL

Lane 1:

Control (vector only transfected HEK-293T lysate)

Lane 2:

POGLUT1 over-expression lysate (mammalian HEK-293T cells)

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human POGLUT1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NBL1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein O-glucosyltransferase 1 (POGLUT1) also known as Rumi or KTELC1 catalyzes the addition of O-linked glucose to serine residues within epidermal growth factor-like (EGF) repeats. This enzyme plays an important role in protein glycosylation. POGLUT1 possesses an approximate molecular mass of 47 kDa. It predominantly exists in the endoplasmic reticulum of cells. Expression of POGLUT1 occurs in various tissues with notable levels in the heart placenta and skeletal muscle.
Biological function summary

This enzyme modifies certain proteins through glycosylation a process vital for normal protein folding and function. POGLUT1 acts independently rather than as part of a larger protein complex. The glycosylation by POGLUT1 affects cell-cell signaling which is essential in developing tissues. It modifies proteins that control cellular signaling pathways providing structural stability and facilitating correct protein interactions.

Pathways

POGLUT1 functions within the Notch signaling pathway. This pathway controls numerous cell differentiation processes impacting development and cell fate decisions. POGLUT1's glycosylation activity enhances Notch receptor maturation influencing its binding affinity and signaling. Perturbations in this pathway can also involve related proteins such as Fringe proteins which adjust Notch signaling through additional glycosylation mechanisms.

Mutations or dysregulation of POGLUT1 link to Dowling-Degos disease a skin condition characterized by reticulate pigmentation. Alterations in POGLUT1 activity may disrupt normal Notch signaling leading to abnormal skin cell differentiation and proliferation. Additionally changes in POGLUT1 function connect to congenital heart defects associating with misregulated Notch signaling and downstream effects on cardiac development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C (PubMed : 21081508, PubMed : 21490058, PubMed : 21949356, PubMed : 27807076, PubMed : 28775322). Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (PubMed : 21081508, PubMed : 21490058, PubMed : 21949356, PubMed : 27807076, PubMed : 28775322). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (PubMed : 27807076). Notch glucosylation does not affect Notch ligand binding (PubMed : 21490058). Required during early development to promote gastrulation : acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (By similarity).
See full target information POGLUT1
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