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Rabbit Recombinant Monoclonal POGZ antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.


Images

Western blot - Anti-POGZ antibody [EPR10612] (AB167408), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-POGZ antibody [EPR10612] (AB167408), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Predicted
Not recommended
Predicted
Predicted
Predicted
Rat
Predicted
Not recommended
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (PubMed:20562864). Promotes the repair of DNA double-strand breaks through the homologous recombination pathway (PubMed:26721387).

Alternative names

KIAA0461, SUHW5, ZNF280E, ZNF635, Nbla00003, POGZ, Pogo transposable element with ZNF domain, Suppressor of hairy wing homolog 5, Zinc finger protein 280E, Zinc finger protein 635

Recommended products

Rabbit Recombinant Monoclonal POGZ antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10612
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

POGZ also known as Pogo Transposable Element with ZNF Domain is a zinc finger protein with a molecular weight of approximately 155 kDa. Scientists have studied POGZ for its role in transcription regulation. This protein localizes primarily in the nucleus and is expressed in various human tissues with higher expression levels observed in the brain. POGZ interacts with chromatin and may play a role in the regulation of gene expression through chromatin remodeling.

Biological function summary

POGZ influences genetic expression by participating in the chromatin modification process. It acts as a member of the protein complex that includes histone modifiers modifying the chromatin landscape to allow or restrict access for transcription machinery. The protein impacts neuronal differentiation and brain development among other processes therefore bearing significance in neurodevelopmental biology. It partners with several key proteins to achieve its biological functions contributing to the regulation of genes required for proper cellular function.

Pathways

More than one biological pathway has involved POGZ. Notably it participates in the chromatin remodeling pathway important for modulating chromatin structure and gene accessibility. POGZ interacts with proteins such as CHD4 part of the NuRD complex within these pathways. These interactions facilitate access to DNA regulating the transcription of genes tied to cellular differentiation and proliferation.

Associated diseases and disorders

Genetics has linked POGZ to neurodevelopmental disorders such as intellectual disability and autism spectrum disorder (ASD). Mutations in the POGZ gene disrupt normal function contributing to these conditions. POGZ has connections with ATRX another protein associated with intellectual disabilities. Research continues to explore how these interactions contribute to the pathogenesis of neurodevelopmental disorders offering potential therapeutic targets in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-POGZ antibody [EPR10612] (ab167408), expandable thumbnail

    Western blot - Anti-POGZ antibody [EPR10612] (ab167408)

    All lanes: Western blot - Anti-POGZ antibody [EPR10612] (ab167408) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: SH-SY5Y cell lysate at 10 µg

    Secondary

    All lanes: HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 155 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)

    ab167408 showing +ve staining in Human normal colon.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)

    ab167408 showing +ve staining in Human breast carcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)

    ab167408 showing +ve staining in Human ovarian carcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-POGZ antibody [EPR10612] (ab167408), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-POGZ antibody [EPR10612] (ab167408)

    Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells, labeling POGZ with ab167408 at 1/10 dilution (red) or a Rabbit IgG negative (green).

  • Immunocytochemistry/ Immunofluorescence - Anti-POGZ antibody [EPR10612] (ab167408), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-POGZ antibody [EPR10612] (ab167408)

    Immunofluorescent staining of HepG2 cells labeling POGZ with ab167408 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling POGZ with ab167408 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling POGZ with ab167408 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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