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AB167408

Anti-POGZ antibody [EPR10612]

4

(3 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal POGZ antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

KIAA0461, SUHW5, ZNF280E, ZNF635, Nbla00003, POGZ, Pogo transposable element with ZNF domain, Suppressor of hairy wing homolog 5, Zinc finger protein 280E, Zinc finger protein 635

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408)

ab167408 showing +ve staining in Human normal colon.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-POGZ antibody [EPR10612] (AB167408)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-POGZ antibody [EPR10612] (AB167408)

Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells, labeling POGZ with ab167408 at 1/10 dilution (red) or a Rabbit IgG negative (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408)

ab167408 showing +ve staining in Human breast carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408)

ab167408 showing +ve staining in Human ovarian carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling POGZ with ab167408 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (AB167408)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling POGZ with ab167408 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-POGZ antibody [EPR10612] (AB167408)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-POGZ antibody [EPR10612] (AB167408)

Immunofluorescent staining of HepG2 cells labeling POGZ with ab167408 at 1/100 dilution.

Western blot - Anti-POGZ antibody [EPR10612] (AB167408)
  • WB

Unknown

Western blot - Anti-POGZ antibody [EPR10612] (AB167408)

All lanes:

Western blot - Anti-POGZ antibody [EPR10612] (ab167408) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 155 kDa

false

  • Carrier free

    Anti-POGZ antibody [EPR10612] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10612

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POGZ also known as Pogo Transposable Element with ZNF Domain is a zinc finger protein with a molecular weight of approximately 155 kDa. Scientists have studied POGZ for its role in transcription regulation. This protein localizes primarily in the nucleus and is expressed in various human tissues with higher expression levels observed in the brain. POGZ interacts with chromatin and may play a role in the regulation of gene expression through chromatin remodeling.
Biological function summary

POGZ influences genetic expression by participating in the chromatin modification process. It acts as a member of the protein complex that includes histone modifiers modifying the chromatin landscape to allow or restrict access for transcription machinery. The protein impacts neuronal differentiation and brain development among other processes therefore bearing significance in neurodevelopmental biology. It partners with several key proteins to achieve its biological functions contributing to the regulation of genes required for proper cellular function.

Pathways

More than one biological pathway has involved POGZ. Notably it participates in the chromatin remodeling pathway important for modulating chromatin structure and gene accessibility. POGZ interacts with proteins such as CHD4 part of the NuRD complex within these pathways. These interactions facilitate access to DNA regulating the transcription of genes tied to cellular differentiation and proliferation.

Genetics has linked POGZ to neurodevelopmental disorders such as intellectual disability and autism spectrum disorder (ASD). Mutations in the POGZ gene disrupt normal function contributing to these conditions. POGZ has connections with ATRX another protein associated with intellectual disabilities. Research continues to explore how these interactions contribute to the pathogenesis of neurodevelopmental disorders offering potential therapeutic targets in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (PubMed : 20562864). Promotes the repair of DNA double-strand breaks through the homologous recombination pathway (PubMed : 26721387).
See full target information POGZ
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 19:20240904 PubMed38463522

2024

Serum levels of HMW adiponectin and its receptors are associated with cytokine levels and clinical characteristics in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Li Lu,Mengyu Cheng

Cell reports 42:112867 PubMed37494184

2023

POGZ suppresses 2C transcriptional program and retrotransposable elements.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Sun,Tianzhe Zhang,Bei Tong,Linxi Cheng,Wei Jiang,Yuhua Sun

Scientific reports 10:635 PubMed31959800

2020

A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dane Z Hazelbaker,Amanda Beccard,Gabriella Angelini,Patrizia Mazzucato,Angelica Messana,Daisy Lam,Kevin Eggan,Lindy E Barrett
View all publications
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Product promise

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